##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631151.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27437
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.783868498742574	31.0	30.0	34.0	27.0	34.0
2	30.983744578488903	31.0	31.0	34.0	27.0	34.0
3	30.418595327477494	31.0	30.0	34.0	26.0	34.0
4	34.55713088165616	35.0	35.0	37.0	30.0	37.0
5	34.704340853591866	35.0	35.0	37.0	32.0	37.0
6	34.94011735976965	35.0	35.0	37.0	32.0	37.0
7	34.82982833400153	35.0	35.0	37.0	32.0	37.0
8	34.874767649524365	35.0	35.0	37.0	32.0	37.0
9	36.7201953566352	39.0	37.0	39.0	32.0	39.0
10	36.52418267303277	38.0	35.0	39.0	32.0	39.0
11	36.56959580129023	38.0	35.0	39.0	32.0	39.0
12	36.390494587600685	38.0	35.0	39.0	32.0	39.0
13	36.574771294237706	39.0	35.0	39.0	32.0	39.0
14	37.20199001348544	39.0	36.0	40.0	31.0	41.0
15	37.283194226774064	39.0	36.0	40.0	32.0	41.0
16	37.18420381237016	39.0	36.0	40.0	31.0	41.0
17	37.02737179720815	39.0	36.0	40.0	31.0	41.0
18	37.38776105259321	39.0	36.0	40.0	32.0	41.0
19	37.16251776797755	39.0	36.0	40.0	31.0	41.0
20	37.27076575427343	39.0	36.0	40.0	31.0	41.0
21	37.17060903159967	39.0	36.0	40.0	31.0	41.0
22	36.90166563399789	38.0	36.0	40.0	31.0	41.0
23	37.15796187629843	39.0	36.0	40.0	31.0	41.0
24	36.81211502715311	38.0	35.0	40.0	30.0	41.0
25	36.644640449028685	38.0	35.0	40.0	30.0	41.0
26	36.33888544665962	38.0	35.0	40.0	30.0	41.0
27	36.024273790866346	38.0	35.0	40.0	30.0	41.0
28	36.26755840653133	38.0	35.0	40.0	30.0	41.0
29	36.113350584976494	38.0	35.0	40.0	30.0	41.0
30	35.86645770310165	38.0	35.0	40.0	29.0	41.0
31	35.76466085942341	38.0	35.0	40.0	29.0	41.0
32	35.398986769690566	38.0	34.0	40.0	27.0	41.0
33	35.2706199657397	38.0	33.0	40.0	27.0	41.0
34	35.22436855341327	38.0	33.0	40.0	27.0	41.0
35	35.18376644676896	38.0	33.0	40.0	27.0	41.0
36	35.18682800597733	38.0	33.0	40.0	26.0	41.0
37	35.09979225133943	38.0	33.0	40.0	26.0	41.0
38	35.0454495753909	38.0	33.0	40.0	26.0	41.0
39	34.74851477931261	38.0	33.0	40.0	24.0	41.0
40	34.157670299230965	37.0	33.0	40.0	23.0	41.0
41	34.27182272114298	37.0	33.0	40.0	23.0	41.0
42	34.08291722855997	37.0	33.0	40.0	23.0	41.0
43	32.74450559463498	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	0.0
12	2.0
13	0.0
14	0.0
15	0.0
16	2.0
17	3.0
18	5.0
19	8.0
20	20.0
21	27.0
22	45.0
23	64.0
24	109.0
25	187.0
26	287.0
27	397.0
28	570.0
29	706.0
30	959.0
31	1122.0
32	1362.0
33	1625.0
34	1975.0
35	2537.0
36	3207.0
37	4028.0
38	5209.0
39	2976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.228159055290302	26.6975252396399	21.263257644786236	31.81105806028356
2	7.252979553158144	16.816707365965666	48.20497867842694	27.72533440244925
3	8.342748842803513	32.853446076466085	46.55027882057076	12.253526260159639
4	5.4816488683165066	26.661078106206947	15.887305463425303	51.969967562051245
5	8.750956737252615	37.154207821554834	33.498560338229396	20.59627510296315
6	23.107482596493785	36.73142107373255	13.000692495535226	27.16040383423844
7	13.215730582789664	39.89867696905638	9.665779786419797	37.21981266173415
8	27.918504209643913	29.325363560155992	27.16040383423844	15.595728395961657
9	11.579254291649962	9.184677625104785	8.889455844297846	70.3466122389474
10	12.085869446368044	29.66796661442578	25.090206655246565	33.155957283959616
11	21.197652804606918	13.879068411269454	26.06698983124977	38.85628895287386
12	11.397018624485185	31.275285198819113	12.39202536720487	44.93567080949084
13	36.70590808032948	9.304953165433538	26.828734919998542	27.16040383423844
14	22.48059190144695	28.683894011735976	13.62029376389547	35.2152203229216
15	28.304843824033238	24.918905128111675	11.426176331231549	35.35007471662354
16	41.03947224550789	16.415788898203157	10.708167802602325	31.83657105368663
17	11.415242191201662	48.948500200459236	11.123665123738018	28.512592484601086
18	30.63746036374239	12.785654408280788	12.741917848161242	43.83496737981557
19	22.684695848671502	37.62802055618326	13.478149943506942	26.2091336516383
20	26.941721033640704	17.046324306593284	14.17793490541969	41.83401975434632
21	40.853591864999814	15.322374895214491	14.094106498523892	29.729926741261796
22	12.993403068848636	49.356708094908335	14.728286620257316	22.92160221598571
23	29.354521266902356	24.05510806575063	15.11098152130335	31.479389146043662
24	39.15515544702409	33.491270911542806	14.389328279330831	12.96424536210227
25	18.300105696686956	45.992637679046545	14.925101140795277	20.782155483471225
26	35.30633815650399	40.09184677625105	14.491380252943106	10.110434814301856
27	22.199948974013196	43.4850748988592	17.669570288296825	16.645405838830776
28	22.247330247476036	22.746655975507526	27.40824434158253	27.597769435433904
29	17.097350293399423	31.91675474723913	17.66592557495353	33.31996938440792
30	26.329409191967052	31.70171665998469	29.485730947260997	12.483143200787259
31	42.100083828406895	18.679155884389694	14.695484200167657	24.525276087035756
32	20.927944017203046	31.085760104967747	22.134344133833874	25.851951743995333
33	9.592885519553887	43.729270692860005	33.59332288515508	13.084520902431024
34	27.05835186062616	37.61344170281007	19.087363778838796	16.24084265772497
35	11.776068812187921	30.305791449502493	48.31432007872581	9.603819659583774
36	21.062798410904982	32.14637168786675	15.77431934978314	31.01651055144513
37	15.100047381273463	28.614644458213363	32.00422786747822	24.281080293034954
38	14.385683565987534	12.559682180996464	37.55148157597405	35.503152677041946
39	33.31267995772132	16.82764150599555	20.355724022305647	29.503954513977476
40	10.569668695557095	11.783358238874513	60.305426978168164	17.341546087400225
41	20.406750009111782	22.360316361118198	18.263658563254	38.96927506651602
42	25.00637824835077	11.750555818784852	45.32565513722346	17.917410795640922
43	22.119765280460694	12.370157087145097	43.025841017603966	22.484236614790245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	9.0
4	16.0
5	16.0
6	27.0
7	38.0
8	38.0
9	38.0
10	57.5
11	77.0
12	77.0
13	122.5
14	168.0
15	382.5
16	597.0
17	555.5
18	514.0
19	514.0
20	488.5
21	463.0
22	347.0
23	231.0
24	205.0
25	179.0
26	179.0
27	156.0
28	133.0
29	146.5
30	160.0
31	168.5
32	177.0
33	177.0
34	207.5
35	238.0
36	244.0
37	250.0
38	252.0
39	254.0
40	254.0
41	288.5
42	323.0
43	361.5
44	400.0
45	536.5
46	673.0
47	673.0
48	1229.5
49	1786.0
50	5256.5
51	8727.0
52	5169.0
53	1611.0
54	1611.0
55	3917.0
56	6223.0
57	4647.0
58	3071.0
59	1783.5
60	496.0
61	496.0
62	389.5
63	283.0
64	214.0
65	145.0
66	116.0
67	87.0
68	87.0
69	64.0
70	41.0
71	30.0
72	19.0
73	13.0
74	7.0
75	7.0
76	5.5
77	4.0
78	3.5
79	3.0
80	2.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	27437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.51029631519481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61791202967673	22.72843240879105
2	8.439321674615792	4.6433647993585305
3	3.245892951775305	2.678864307322229
4	1.377848436671966	1.5162007508109487
5	1.232114467408585	1.6947917046324306
6	0.5696873343932167	0.9403360425702518
7	0.5299417064122947	1.020519736122754
8	0.3179650238473768	0.6997849619127455
9	0.23847376788553257	0.590443561613879
>10	1.192368839427663	5.53631956846594
>50	0.06624271330153683	1.2792943834967379
>100	0.09273979862215156	7.107191019426322
>500	0.026497085320614733	6.057513576557204
>1k	0.052994170641229466	43.50694317891898
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	4035	14.706418340197544	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	3136	11.429821044574844	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	3066	11.174691110544156	RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	1700	6.196012683602435	No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	847	3.087072201771331	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	815	2.970441374785873	RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	402	1.465174764004811	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	364	1.3266756569595801	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	312	1.1371505631082115	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	246	0.8965994824507053	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	238	0.8674417757043409	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	204	0.7435215220322922	No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	184	0.6706272551663812	No Hit
CTGATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	76	0.27699821409046177	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	75	0.27335350074716624	No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT	72	0.2624193607172796	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	67	0.24419579400080182	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61	0.2223275139410285	No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	48	0.17494624047818638	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	44	0.1603673871050042	TruSeq Adapter, Index 25 (96% over 27bp)
TCGCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	42	0.1530779604184131	RNA PCR Primer, Index 17 (95% over 21bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	40	0.145788533731822	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	36	0.1312096803586398	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	31	0.11298611364216206	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	31	0.11298611364216206	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	29	0.10569668695557094	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	29	0.10569668695557094	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	28	0.1020519736122754	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	28	0.1020519736122754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.00364471334329555	0.0
12	0.0	0.0	0.0	0.00364471334329555	0.0
13	0.0	0.0	0.0	0.00364471334329555	0.0
14	0.0	0.0	0.0	0.00364471334329555	0.0
15	0.0	0.0	0.0	0.0072894266865911	0.0
16	0.0	0.0	0.0	0.0072894266865911	0.0
17	0.0	0.0	0.0	0.0072894266865911	0.0
18	0.0	0.0	0.0	0.0072894266865911	0.0
19	0.0	0.0	0.0	0.0072894266865911	0.0
20	0.0	0.0	0.0	0.0072894266865911	0.0
21	0.0	0.0	0.0	0.0072894266865911	0.0
22	0.0	0.0	0.0	0.0145788533731822	0.0
23	0.0	0.0	0.0	0.01822356671647775	0.0
24	0.0	0.0	0.0	0.02551299340306885	0.0
25	0.0	0.0	0.0	0.02551299340306885	0.0
26	0.0	0.0	0.0	0.032802420089659945	0.0
27	0.0	0.0	0.0	0.0364471334329555	0.0
28	0.0	0.0	0.0	0.04009184677625105	0.0
29	0.0	0.0	0.0	0.07653898020920655	0.0
30	0.0	0.0	0.0	0.13485439370193533	0.0
31	0.0	0.0	0.0	0.17494624047818638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTTGA	25	1.20245924E-4	37.0	25
GCTTGAA	25	1.20245924E-4	37.0	26
CTGCTTG	25	1.20245924E-4	37.0	24
TTGAAAA	25	1.20245924E-4	37.0	28
CTTGAAA	25	1.20245924E-4	37.0	27
CTTATAC	485	0.0	36.237114	1
TTATACA	500	0.0	35.149998	2
TATACAC	535	0.0	33.196262	3
TCTGCTT	30	3.5093876E-4	30.833332	23
TGAAAAA	30	3.5093876E-4	30.833332	29
TTCTGCT	35	8.6494756E-4	26.42857	22
TCTTCTG	35	8.6494756E-4	26.42857	20
CTTCTGC	35	8.6494756E-4	26.42857	21
CCGTCTT	480	0.0	25.822916	37
GCCGTCT	500	0.0	24.789999	36
GGTATCA	90	1.2551027E-10	24.666666	1
CGTCTTC	145	0.0	24.241379	37
TACACAT	890	0.0	20.786518	5
ATACACA	875	0.0	20.50857	4
TGCCGTC	610	0.0	20.319672	35
>>END_MODULE