FastQCFastQC Report
Fri 10 Feb 2017
ERR1631150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460800
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG168863.6644965277777777No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT138393.0032552083333335TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC95442.071180555555556No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT86111.8687065972222223No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC51101.1089409722222223RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC45040.9774305555555555TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG24000.5208333333333333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21060.45703125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.43684895833333337No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA17420.3780381944444444No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA13090.2840711805555556No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT12730.2762586805555555No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT12170.2641059027777778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8450.1833767361111111No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6110.1325954861111111No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT4630.10047743055555557TruSeq Adapter, Index 11 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11100.033.8333321
CGTCTTC6350.033.79527337
ATGCCGG250.0054929729.59999836
CGCCGAG250.0054929729.5999982
TGCCGGC250.0054929729.59999837
TTATACA13300.028.6541372
GATCAAC1050.028.1904753
GCAACGC405.932527E-527.753
GGTATCA3650.027.3698641
TATACAC15200.025.43753
CCGTCTT20300.025.33497637
AAACGTA451.3215323E-424.66666615
GCCGTCT21050.024.43230436
GTATCAA10250.024.0048771
CGTCAAT400.001929441523.12530
GTGACAC753.730711E-722.224
GATACAC603.7200538E-521.5833323
ATAGCAT705.0909857E-621.14285730
AACGGCC806.941518E-720.812537
GGGATCA1251.8189894E-1120.721
Produced by FastQC (version 0.11.5)