##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	460800
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.079891493055555	31.0	30.0	31.0	26.0	34.0
2	30.0562890625	31.0	30.0	31.0	26.0	34.0
3	29.525846354166667	31.0	30.0	31.0	25.0	34.0
4	33.74099609375	35.0	33.0	37.0	28.0	37.0
5	33.28561197916667	35.0	33.0	37.0	28.0	37.0
6	33.60474392361111	35.0	33.0	37.0	28.0	37.0
7	33.69898871527778	35.0	33.0	37.0	29.0	37.0
8	33.77049262152778	35.0	33.0	37.0	29.0	37.0
9	35.32366753472222	37.0	34.0	39.0	30.0	39.0
10	34.95212673611111	37.0	33.0	39.0	29.0	39.0
11	35.15050998263889	37.0	34.0	39.0	30.0	39.0
12	35.010442708333336	37.0	34.0	39.0	29.0	39.0
13	35.21283637152778	37.0	34.0	39.0	30.0	39.0
14	35.898967013888885	38.0	34.0	40.0	29.0	41.0
15	35.887836371527776	38.0	34.0	40.0	29.0	41.0
16	35.90894314236111	38.0	34.0	40.0	29.0	41.0
17	35.790049913194444	38.0	34.0	40.0	29.0	41.0
18	35.927486979166666	38.0	34.0	40.0	29.0	41.0
19	35.908283420138886	38.0	34.0	40.0	29.0	41.0
20	35.878582899305556	38.0	34.0	40.0	29.0	41.0
21	35.77147569444445	38.0	34.0	40.0	29.0	41.0
22	35.52886501736111	38.0	34.0	40.0	27.0	40.0
23	35.63037326388889	38.0	34.0	40.0	28.0	40.0
24	35.51012369791667	38.0	34.0	40.0	27.0	41.0
25	35.46760416666667	38.0	34.0	40.0	27.0	41.0
26	35.27631293402778	38.0	34.0	40.0	27.0	40.0
27	35.065403645833335	37.0	33.0	40.0	27.0	40.0
28	35.035060763888886	37.0	33.0	39.0	27.0	40.0
29	34.946477864583336	37.0	33.0	39.0	27.0	40.0
30	34.8524609375	37.0	33.0	39.0	26.0	40.0
31	34.84336588541667	37.0	33.0	39.0	26.0	40.0
32	34.65765625	37.0	33.0	39.0	26.0	40.0
33	34.61798611111111	37.0	33.0	39.0	25.0	40.0
34	34.51697699652778	37.0	33.0	39.0	25.0	40.0
35	34.47041232638889	37.0	33.0	39.0	25.0	40.0
36	34.53868706597222	37.0	33.0	39.0	25.0	40.0
37	34.4269140625	37.0	33.0	39.0	25.0	40.0
38	34.32800130208334	37.0	33.0	39.0	25.0	40.0
39	34.201436631944446	37.0	32.0	39.0	24.0	40.0
40	33.91842447916667	37.0	32.0	39.0	24.0	40.0
41	33.98103949652778	37.0	32.0	39.0	24.0	40.0
42	33.88462673611111	37.0	32.0	39.0	24.0	40.0
43	32.966334635416665	36.0	30.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	17.0
11	20.0
12	17.0
13	19.0
14	10.0
15	17.0
16	33.0
17	52.0
18	96.0
19	148.0
20	298.0
21	537.0
22	975.0
23	1612.0
24	2634.0
25	4132.0
26	5918.0
27	8473.0
28	11667.0
29	15697.0
30	20446.0
31	25717.0
32	32313.0
33	40144.0
34	48234.0
35	56883.0
36	61877.0
37	58248.0
38	45929.0
39	18624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.90234375	25.305338541666668	17.19769965277778	22.594618055555554
2	14.646918402777779	22.897352430555557	38.701171875	23.754557291666668
3	16.134331597222225	30.425998263888886	33.873046875	19.56662326388889
4	11.64822048611111	19.5078125	34.850911458333336	33.99305555555556
5	11.940755208333332	39.56749131944444	33.638454861111114	14.85329861111111
6	26.344835069444443	39.59613715277778	16.84223090277778	17.216796875
7	22.655381944444443	35.370876736111114	20.982421875	20.991319444444446
8	26.132812500000004	31.80121527777778	23.87152777777778	18.194444444444443
9	22.545789930555554	14.029947916666666	17.93988715277778	45.484375
10	14.570095486111112	27.491102430555554	32.625	25.313802083333332
11	30.64822048611111	22.426866319444443	23.27170138888889	23.653211805555554
12	19.586371527777775	28.505425347222225	25.806640625	26.101562499999996
13	31.78667534722222	19.10177951388889	24.287760416666668	24.82378472222222
14	22.171006944444443	24.324869791666668	25.03298611111111	28.471137152777775
15	25.53927951388889	29.07074652777778	20.97612847222222	24.41384548611111
16	25.23654513888889	26.512152777777775	21.960069444444443	26.29123263888889
17	20.13259548611111	32.001302083333336	22.679036458333332	25.18706597222222
18	24.62087673611111	23.20464409722222	24.138888888888886	28.03559027777778
19	28.655598958333332	27.33550347222222	23.77191840277778	20.236979166666664
20	25.397786458333332	25.902994791666668	23.537977430555557	25.161241319444443
21	28.307942708333332	27.00238715277778	23.58203125	21.10763888888889
22	23.806640625	31.411241319444443	24.33441840277778	20.44769965277778
23	24.512369791666664	24.03515625	25.48611111111111	25.966362847222225
24	24.823133680555557	28.56792534722222	27.073133680555557	19.535807291666664
25	22.330078125	29.890190972222225	23.80316840277778	23.9765625
26	26.12109375	27.157335069444443	24.676649305555557	22.044921875
27	25.84592013888889	29.115885416666664	25.241102430555557	19.79709201388889
28	19.880208333333332	27.363498263888893	27.73025173611111	25.026041666666664
29	26.18315972222222	25.37934027777778	26.821614583333332	21.615885416666668
30	22.335503472222225	25.904296875	27.63910590277778	24.12109375
31	21.44683159722222	25.57378472222222	27.775824652777775	25.20355902777778
32	23.93923611111111	29.305338541666664	27.035807291666668	19.719618055555554
33	18.680121527777775	24.887586805555557	30.20551215277778	26.226779513888886
34	20.668836805555554	29.68576388888889	27.92730034722222	21.718098958333336
35	20.199869791666668	25.791449652777775	34.06879340277777	19.93988715277778
36	23.11284722222222	24.63650173611111	24.358506944444443	27.89214409722222
37	21.831380208333336	24.979383680555557	29.823133680555557	23.366102430555554
38	21.856553819444443	23.11501736111111	28.600694444444446	26.427734375
39	26.971571180555554	24.688585069444443	26.131727430555557	22.208116319444443
40	20.10915798611111	22.876953125	35.31987847222222	21.694010416666668
41	22.98220486111111	25.863281249999996	25.12065972222222	26.033854166666664
42	21.046006944444446	23.31293402777778	33.04991319444445	22.591145833333336
43	24.02756076388889	23.00173611111111	29.709418402777775	23.26128472222222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	13.0
2	16.0
3	51.0
4	86.0
5	86.0
6	132.0
7	178.0
8	190.0
9	202.0
10	291.0
11	380.0
12	380.0
13	627.5
14	875.0
15	2078.0
16	3281.0
17	3617.0
18	3953.0
19	3953.0
20	3845.5
21	3738.0
22	4213.0
23	4688.0
24	6035.5
25	7383.0
26	7383.0
27	8788.5
28	10194.0
29	12055.5
30	13917.0
31	15920.0
32	17923.0
33	17923.0
34	19580.5
35	21238.0
36	22738.5
37	24239.0
38	25007.0
39	25775.0
40	25775.0
41	26590.5
42	27406.0
43	27902.5
44	28399.0
45	28930.5
46	29462.0
47	29462.0
48	29499.0
49	29536.0
50	30063.0
51	30590.0
52	41867.0
53	53144.0
54	53144.0
55	39398.5
56	25653.0
57	34427.5
58	43202.0
59	34752.5
60	26303.0
61	26303.0
62	18636.5
63	10970.0
64	9176.0
65	7382.0
66	6079.5
67	4777.0
68	4777.0
69	3804.0
70	2831.0
71	2345.0
72	1859.0
73	1280.0
74	701.0
75	701.0
76	482.0
77	263.0
78	191.5
79	120.0
80	90.5
81	61.0
82	61.0
83	44.5
84	28.0
85	23.5
86	19.0
87	16.0
88	13.0
89	13.0
90	8.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	460800.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.09821900874558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.03745512426441	43.96360724894783
2	7.961313082025291	8.136178389250393
3	2.0227832314123573	3.1008186169778016
4	0.9129711987708072	1.8660480905387082
5	0.5043568632562542	1.288586872861601
6	0.39233694532101	1.2028631494341127
7	0.2894897770807672	1.0354688421046188
8	0.2172526908506642	0.888098046186123
9	0.17234151941967138	0.7925710233245713
>10	1.3016711113667372	13.14398491187749
>50	0.12916369178115583	4.540391022941885
>100	0.052512451740331874	4.458810267249999
>500	8.469750280698688E-4	0.3150705046889589
>1k	0.0033879001122794753	3.5843597799917006
>5k	0.0012704625421048033	5.034419843124058
>10k+	8.469750280698688E-4	6.64872339050018
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	16886	3.6644965277777777	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	13839	3.0032552083333335	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	9544	2.071180555555556	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	8611	1.8687065972222223	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	5110	1.1089409722222223	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	4504	0.9774305555555555	TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	2400	0.5208333333333333	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2106	0.45703125	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2013	0.43684895833333337	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1742	0.3780381944444444	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1309	0.2840711805555556	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	1273	0.2762586805555555	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	1217	0.2641059027777778	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.1833767361111111	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	611	0.1325954861111111	No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT	463	0.10047743055555557	TruSeq Adapter, Index 11 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	2.1701388888888888E-4	0.0
20	0.0	0.0	0.0	4.3402777777777775E-4	0.0
21	0.0	0.0	0.0	6.510416666666666E-4	0.0
22	0.0	0.0	0.0	8.680555555555555E-4	0.0
23	0.0	0.0	2.1701388888888888E-4	0.001953125	0.0
24	0.0	0.0	2.1701388888888888E-4	0.001953125	0.0
25	0.0	0.0	2.1701388888888888E-4	0.001953125	0.0
26	0.0	0.0	2.1701388888888888E-4	0.002387152777777778	0.0
27	0.0	0.0	2.1701388888888888E-4	0.0030381944444444445	0.0
28	0.0	0.0	2.1701388888888888E-4	0.004991319444444444	0.0
29	0.0	0.0	2.1701388888888888E-4	0.012152777777777778	0.0
30	0.0	0.0	2.1701388888888888E-4	0.022786458333333332	0.0
31	0.0	0.0	2.1701388888888888E-4	0.050347222222222224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1110	0.0	33.833332	1
CGTCTTC	635	0.0	33.795273	37
ATGCCGG	25	0.00549297	29.599998	36
CGCCGAG	25	0.00549297	29.599998	2
TGCCGGC	25	0.00549297	29.599998	37
TTATACA	1330	0.0	28.654137	2
GATCAAC	105	0.0	28.190475	3
GCAACGC	40	5.932527E-5	27.75	3
GGTATCA	365	0.0	27.369864	1
TATACAC	1520	0.0	25.4375	3
CCGTCTT	2030	0.0	25.334976	37
AAACGTA	45	1.3215323E-4	24.666666	15
GCCGTCT	2105	0.0	24.432304	36
GTATCAA	1025	0.0	24.004877	1
CGTCAAT	40	0.0019294415	23.125	30
GTGACAC	75	3.730711E-7	22.2	24
GATACAC	60	3.7200538E-5	21.583332	3
ATAGCAT	70	5.0909857E-6	21.142857	30
AACGGCC	80	6.941518E-7	20.8125	37
GGGATCA	125	1.8189894E-11	20.72	1
>>END_MODULE