FastQCFastQC Report
Fri 10 Feb 2017
ERR1631149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396095
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT92222.328229338921218TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG91322.3055075171360406No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT53811.3585124780671305No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC47881.2088009189714588No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC35590.898521819260531RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC24600.6210631287948598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.35900478420580917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14010.3537030257892677No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG11140.28124566076320073No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA8550.2158573069591891No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT7670.19364041454701522No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT6430.16233479342077028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5510.13910804226258852No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4170.10527777427132379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7250.033.4275861
CGTCTTC4750.031.93684237
TTATACA7950.030.0188672
TAACGCA250.005492049329.59999815
TAGTTCG250.005492049329.5999989
AACGCAT250.005492049329.59999816
CCGGTCG250.005492049329.5999989
CGGTCGT250.005492049329.59999810
GTGCGGG250.005492049329.5999981
GGTATCA2650.028.6226431
TATACAC9350.026.909093
GTTAACG358.859091E-426.42857213
CCGTCTT15050.025.19933337
GCCGTCT15300.024.78758236
GCCACGT903.808964E-922.61111333
TGCCGTC17450.021.73352235
ATGCCGT17900.021.18715134
GTATCAA6550.021.1832051
TCAATAC450.00382144720.5555573
TGCGTTT450.00382144720.55555724
Produced by FastQC (version 0.11.5)