FastQCFastQC Report
Fri 10 Feb 2017
ERR1631148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730949
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34650.47404128058181894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33080.4525623538714739No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG32910.4502366102149397No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT31580.4320410863138194No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT21300.2914019993186939No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC20420.27936285568486996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11260.15404631513279313No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC9590.13119930391860443No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT8350.11423505607094338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCC352.3871276E-531.7142856
TAATACG502.7184979E-729.5999984
TTAACGG454.005631E-628.77777935
GGTATCA3600.028.263891
CTTATAC4800.026.5937521
GTATCAA12050.026.0995851
GCACCGC509.085179E-625.89999810
AATACGC509.085179E-625.8999985
GATTACG751.3720637E-824.6666661
CCGTCTT3400.024.48529437
GCCGTCT3350.024.29850636
CGTCTTC1300.024.19230837
AACGGCC551.9014304E-523.54545437
GTGCGCA400.00193051823.1259
CCGTTAT652.6806265E-622.769239
CGTTATA603.723897E-521.58333410
GTATTAG2250.020.5555551
TATACAC6350.020.3937023
TACAGCG555.140632E-420.1818185
TAACGGC656.897735E-519.92307736
Produced by FastQC (version 0.11.5)