##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631148.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	730949
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56035646809832	31.0	31.0	34.0	30.0	34.0
2	31.55558595743342	31.0	31.0	34.0	30.0	34.0
3	31.02860254272186	31.0	31.0	34.0	27.0	34.0
4	35.182100255968614	37.0	35.0	37.0	32.0	37.0
5	35.38000188795662	37.0	35.0	37.0	33.0	37.0
6	35.56328827319006	37.0	35.0	37.0	33.0	37.0
7	35.57763947963538	37.0	35.0	37.0	33.0	37.0
8	35.60000218893521	37.0	35.0	37.0	33.0	37.0
9	37.35927403963888	39.0	37.0	39.0	34.0	39.0
10	37.20196484296442	39.0	37.0	39.0	33.0	39.0
11	37.26260382051279	39.0	37.0	39.0	34.0	39.0
12	37.212503197897526	39.0	37.0	39.0	34.0	39.0
13	37.27209969505397	39.0	37.0	39.0	34.0	39.0
14	38.44433469366536	40.0	38.0	41.0	34.0	41.0
15	38.44957445731508	40.0	38.0	41.0	34.0	41.0
16	38.463984491394065	40.0	38.0	41.0	34.0	41.0
17	38.46069698433133	40.0	38.0	41.0	34.0	41.0
18	38.488258414745765	40.0	38.0	41.0	34.0	41.0
19	38.53027776219682	40.0	38.0	41.0	34.0	41.0
20	38.50373692282225	40.0	38.0	41.0	34.0	41.0
21	38.47464734201702	40.0	38.0	41.0	34.0	41.0
22	38.41394953683499	40.0	38.0	41.0	34.0	41.0
23	38.39245008885709	40.0	38.0	41.0	34.0	41.0
24	38.366980459649035	40.0	38.0	41.0	34.0	41.0
25	38.34741137890605	40.0	38.0	41.0	34.0	41.0
26	38.25726281860978	40.0	38.0	41.0	34.0	41.0
27	38.18714164736527	40.0	38.0	41.0	34.0	41.0
28	38.14887632379277	40.0	38.0	41.0	33.0	41.0
29	38.06490740120036	40.0	37.0	41.0	33.0	41.0
30	37.98358845829189	40.0	37.0	41.0	33.0	41.0
31	37.9463232044917	40.0	37.0	41.0	33.0	41.0
32	37.84900998564879	40.0	37.0	41.0	33.0	41.0
33	37.855756010337245	40.0	37.0	41.0	33.0	41.0
34	37.79084040062987	40.0	37.0	41.0	33.0	41.0
35	37.738591885343574	40.0	37.0	41.0	33.0	41.0
36	37.7145067576534	40.0	37.0	41.0	33.0	41.0
37	37.66134573000305	40.0	37.0	41.0	33.0	41.0
38	37.61961915263582	40.0	37.0	41.0	33.0	41.0
39	37.54530617047154	40.0	37.0	41.0	32.0	41.0
40	37.50235378938886	40.0	37.0	41.0	32.0	41.0
41	37.43917838317037	40.0	37.0	41.0	32.0	41.0
42	37.38714055289767	40.0	37.0	41.0	32.0	41.0
43	36.803902871472566	39.0	36.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	6.0
11	4.0
12	4.0
13	3.0
14	1.0
15	3.0
16	1.0
17	6.0
18	10.0
19	14.0
20	59.0
21	127.0
22	207.0
23	410.0
24	782.0
25	1413.0
26	2251.0
27	3380.0
28	5155.0
29	7619.0
30	10742.0
31	14474.0
32	19067.0
33	25423.0
34	34087.0
35	46762.0
36	66046.0
37	99926.0
38	178219.0
39	214746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.73457655732479	23.315032922953584	15.547733152381356	22.402657367340264
2	13.660460579329065	23.68427893054098	40.97618301687259	21.67907747325737
3	14.746309250029757	29.07124847287567	34.507195440447965	21.675246836646604
4	11.565375970142924	18.979983555624262	41.42628281863714	28.02835765559567
5	11.56865937295215	39.67130401710653	36.50583009211313	12.254206517828194
6	26.217697814758623	42.24343969278294	18.43151847803335	13.107344014425081
7	23.58728173921847	34.2216762044958	24.800772694127772	17.390269362157962
8	23.952560301744718	36.32715825591115	22.423041826447538	17.297239615896594
9	24.779977809669347	15.799597509538968	21.490008194826178	37.93041648596551
10	13.701776731345142	29.716847550239482	35.44419651713047	21.137179201284905
11	31.036775479547824	24.81459034761659	26.206069096475954	17.94256507635964
12	20.433573340958127	27.457319183691336	31.922883812687342	20.186223662663195
13	27.13595613373847	22.897083107029356	28.101686985001688	21.865273774230488
14	20.80528190065244	22.78722592137071	29.554866344984397	26.852625832992455
15	22.036694762562096	30.364635562809443	25.529551309325278	22.06911836530319
16	21.34088698390722	30.19047840547015	26.102368291084606	22.366266319538024
17	20.438908870523115	29.807688361294698	27.959132579701183	21.794270188481
18	21.70383980277694	28.234391181874525	29.032531681416895	21.02923733393164
19	22.709518721552392	27.8443502898287	29.551719750625555	19.89441123799335
20	22.56299687119074	27.587560828457253	28.89654408173484	20.952898218617165
21	22.303471240811604	28.001269582419567	28.639207386561853	21.05605179020698
22	20.53316989283794	29.54843634781633	29.11023888123522	20.80815487811051
23	21.27850233053195	27.692356101451676	29.455817026906118	21.573324541110257
24	21.34211825996068	28.522099353032836	29.28754263293335	20.84823975407313
25	21.332268051533006	27.983074058518447	29.199985224687357	21.484672665261186
26	21.508750952528835	28.185960990438456	29.247594565421114	21.05769349161159
27	20.65137239397003	29.135958869907476	28.71253671596787	21.500132020154624
28	21.056188598657364	28.104286345558993	29.451575964944205	21.38794909083944
29	21.378782924663692	28.60651016691999	29.205183945801966	20.809522962614356
30	20.743444481078708	28.397193237831914	29.45964766351688	21.3997146175725
31	22.34164079846884	27.868565385546734	28.774237327091218	21.015556488893207
32	20.727164275482966	28.562731462796993	29.892235983632236	20.8178682780878
33	20.541515208311388	27.818083067354905	30.007018273504716	21.63338345082899
34	21.30627444595998	28.231655012866835	28.931430236582855	21.530640304590335
35	20.657665582687713	28.218247784729165	30.61444779321129	20.50963883937183
36	21.464698631505073	28.126038889170108	28.458893848955263	21.95036863036956
37	21.567441777743728	27.743796078796194	29.410807046729666	21.277955096730413
38	21.477421817390816	27.66800419728326	29.41422725798927	21.440346727336653
39	21.540764129918777	28.020559573923766	29.24827860767304	21.19039768848442
40	20.670799193924612	27.76349649565154	30.524838258209535	21.040866052214312
41	21.23773341231741	27.64611484522176	29.017893177225773	22.098258565235056
42	21.064397105680424	27.604935501656065	29.678404375681477	21.652263016982033
43	21.282743392493867	26.999147683354106	30.07952675220843	21.638582171943597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	25.5
2	34.0
3	147.5
4	261.0
5	261.0
6	317.5
7	374.0
8	385.5
9	397.0
10	560.0
11	723.0
12	723.0
13	1173.0
14	1623.0
15	3819.0
16	6015.0
17	6871.0
18	7727.0
19	7727.0
20	8464.5
21	9202.0
22	11517.0
23	13832.0
24	17112.5
25	20393.0
26	20393.0
27	23819.5
28	27246.0
29	31651.5
30	36057.0
31	39567.0
32	43077.0
33	43077.0
34	45224.5
35	47372.0
36	50125.0
37	52878.0
38	53603.5
39	54329.0
40	54329.0
41	54773.5
42	55218.0
43	53124.5
44	51031.0
45	50252.0
46	49473.0
47	49473.0
48	47481.5
49	45490.0
50	44223.5
51	42957.0
52	45231.0
53	47505.0
54	47505.0
55	40515.0
56	33525.0
57	31700.5
58	29876.0
59	24999.0
60	20122.0
61	20122.0
62	16662.5
63	13203.0
64	10931.0
65	8659.0
66	6928.0
67	5197.0
68	5197.0
69	4127.5
70	3058.0
71	2563.5
72	2069.0
73	1593.0
74	1117.0
75	1117.0
76	794.5
77	472.0
78	350.5
79	229.0
80	164.5
81	100.0
82	100.0
83	75.0
84	50.0
85	39.0
86	28.0
87	17.5
88	7.0
89	7.0
90	6.5
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	730949.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.876616919973735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.35137259297495	50.506748234235324
2	9.877756686953049	11.828933063467932
3	2.334058171874494	4.192665210787898
4	0.9255692902856626	2.2167983130930633
5	0.49221014282554737	1.4735939083045428
6	0.32075593612515196	1.1523468187303976
7	0.2088802776261882	0.8754931055892722
8	0.17951842873801968	0.8599164950097606
9	0.13812946204423587	0.7443652395766286
>10	0.9970310183593617	11.88291368807418
>50	0.10776587111526012	4.4593302987947485
>100	0.06351279979004086	6.523881078211959
>500	0.001834304686961741	0.7404046939843758
>1k	0.0016050166010915235	2.54260985213993
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3465	0.47404128058181894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3308	0.4525623538714739	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3291	0.4502366102149397	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	3158	0.4320410863138194	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2130	0.2914019993186939	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	2042	0.27936285568486996	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1126	0.15404631513279313	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	959	0.13119930391860443	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	835	0.11423505607094338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.3680845038436333E-4	0.0
18	0.0	0.0	0.0	1.3680845038436333E-4	0.0
19	0.0	0.0	0.0	2.7361690076872666E-4	0.0
20	0.0	0.0	0.0	2.7361690076872666E-4	0.0
21	0.0	0.0	0.0	9.576591526905434E-4	0.0
22	0.0	0.0	0.0	0.0015048929542279968	0.0
23	0.0	0.0	0.0	0.0019153183053810868	0.0
24	0.0	0.0	0.0	0.0023257436565341768	0.0
25	0.0	0.0	0.0	0.0027361690076872667	0.0
26	0.0	0.0	0.0	0.00287297745807163	0.0
27	0.0	0.0	0.0	0.0034202112596090836	0.0
28	0.0	0.0	0.0	0.007524464771139984	0.0
29	1.3680845038436333E-4	0.0	0.0	0.014638504191126878	0.0
30	1.3680845038436333E-4	0.0	0.0	0.029961050634175573	0.0
31	1.3680845038436333E-4	0.0	0.0	0.061563802672963505	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGCC	35	2.3871276E-5	31.714285	6
TAATACG	50	2.7184979E-7	29.599998	4
TTAACGG	45	4.005631E-6	28.777779	35
GGTATCA	360	0.0	28.26389	1
CTTATAC	480	0.0	26.593752	1
GTATCAA	1205	0.0	26.099585	1
GCACCGC	50	9.085179E-6	25.899998	10
AATACGC	50	9.085179E-6	25.899998	5
GATTACG	75	1.3720637E-8	24.666666	1
CCGTCTT	340	0.0	24.485294	37
GCCGTCT	335	0.0	24.298506	36
CGTCTTC	130	0.0	24.192308	37
AACGGCC	55	1.9014304E-5	23.545454	37
GTGCGCA	40	0.001930518	23.125	9
CCGTTAT	65	2.6806265E-6	22.76923	9
CGTTATA	60	3.723897E-5	21.583334	10
GTATTAG	225	0.0	20.555555	1
TATACAC	635	0.0	20.393702	3
TACAGCG	55	5.140632E-4	20.181818	5
TAACGGC	65	6.897735E-5	19.923077	36
>>END_MODULE