FastQCFastQC Report
Fri 10 Feb 2017
ERR1631147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457887
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT234765.127029157848989No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG202984.432971453655597No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT154523.3746317322832926TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC106092.316947194395124No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC69421.5160945822877696RNA PCR Primer, Index 1 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69311.5136922428459423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66651.455599307252663No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC51471.1240764642804884No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA47521.0378106388694153No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA29610.6466660988409805No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT27070.5911938971842398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25860.5647681633241389No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25000.5459862367789433No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT23650.5165029799928803No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG22940.5009969708683584No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT21110.46103077833613965No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA20680.4516398150635419No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC20130.43962811785440514No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG19580.4276164206452684No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC18250.39856995284862856No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG17870.39027096204958867No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT16570.3618796777370836No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG13780.3009476137125535No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG13220.2887175220087052No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG12830.2802001367149537No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12540.27386669636831795No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG12430.2714643569264906No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12380.2703723844529327No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT12340.26949880647408636No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG11980.2616366046644696No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11730.2561767422966802No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11210.24482022857167818No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG11050.24132591665629294No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10750.23477408181494563No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10480.22887743045773304No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG10030.21904967819571203No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10030.21904967819571203No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9490.2072563754812869No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9370.20463564154474795No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT9330.20376206356590162No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC9320.20354366907119006No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.18214100858945548No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT8210.17930188015820497No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8200.1790834856634934No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7870.17187646733801135No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG7730.16881894441204925No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG7480.16335908204425983No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC7350.16051995361300933No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7270.1587727976553167No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA7250.15833600866589354No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7040.1537497242769504No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG7030.15353132978223885No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7030.15353132978223885No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA7010.15309454079281568No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6890.15047380685627676No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6700.1463243114567568No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6620.14457715549906416No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6340.13846210964714004No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6270.13693334818415898No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG6220.1358413757106011No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT6110.13343903626877374No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6080.132783852784639No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6030.13169188031108112No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.1292895408692538No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5870.12819756839569588No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5860.12797917390098432No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5700.12448486198559908No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5680.12404807299617591No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC5630.12295610052261803No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA5550.1212089445649254No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA5540.12099055007021382No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA5520.12055376108079066No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5510.12033536658607911No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC5410.11815142163896332No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG5370.11727784366011701No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT5330.1164042656812707RNA PCR Primer, Index 40 (95% over 22bp)
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA5200.1135651372500202No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA5160.11269155927117389No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5050.11028921982934654No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4930.10766848589280761No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4900.10701330240867288No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4830.10548454094569185No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG4820.10526614645098027No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4780.10439256847213395No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4700.10264541251444133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG403.824789E-837.01
TTACTTT403.824789E-837.03
TAGCAAC650.037.01
TTGACGG200.001840779637.019
TCAGAGT551.2732926E-1137.03
GTATACA200.001840779637.02
GAATTGA200.001840779637.016
TAGTTTA251.2324659E-436.99999611
TAGCAGT251.2324659E-436.9999965
CTTAGCA251.2324659E-436.9999963
GTAGTTT251.2324659E-436.99999610
CTTATAC26250.035.1676181
ATTACGG1000.035.1499982
TTACGGG1000.035.1499983
AGCAACG700.034.3571432
GACAGAC507.3123374E-933.316
ACAGACA507.3123374E-933.317
GATTACG1000.033.31
CGTCTTC8450.033.27810337
CCTAGTT451.0775511E-732.888892
Produced by FastQC (version 0.11.5)