FastQCFastQC Report
Fri 10 Feb 2017
ERR1631146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719179
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT154342.146058213601899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT149712.081679248142674No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT113731.5813865532781128No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG94651.3160840347118035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA75821.0542577021854087No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT59520.8276103723829533RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC54980.7644828338980977No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA52300.7272181195502093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51140.711088616325004No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT43620.606524940244362No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA38050.5290755152750567No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT34610.4812431953658268No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG32340.449679426123399No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT31730.4411975321860066No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC31260.43466230243096643No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC29400.40879947829399915No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG27480.38210236950745224No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC26690.37111762162132095RNA PCR Primer, Index 29 (95% over 21bp)
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG25730.3577690672280475No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG22670.31522055009948846No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT21350.2968662878087375No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG21170.2943634338599987No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC21140.2939462915352089RNA PCR Primer, Index 29 (95% over 24bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG20490.28490820783143No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG20310.2824053538826912No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC20220.28115392690832186No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG19740.27447964971168515No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG18650.2593234785776559No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.25821109904488315No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA18190.2529272962642124No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG17970.2498682525490872No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG17930.24931206278270085No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG17080.23749303024698998No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA16950.23568541350623418No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG16930.23540731862304098No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG16160.2247006656201029No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA15440.21468924982514784No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT15410.21427210750035805No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC14700.20439973914699958No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.19953307869111864No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG14270.19842069915834581No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT14080.19577879776801047No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA13920.1935540387024649No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA13120.18243024337473704No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13080.18187405360835063No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG12400.17241882757978194No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG12100.168247404331884No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC12040.1674131196823044No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG11950.16616169270793502No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11910.16560550294154863No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA11800.16407598108398605No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG11750.16338074387600307No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG11460.1593483680697017No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG11450.1592093206281051No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11380.1582359885369289No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11340.15767979877054253No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA11010.15309123319785478No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA10890.1514226638986956No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG10860.15100552157390582No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10820.1504493318075194No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC10720.14905885739155342No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT10360.14405314949407588No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG10180.1415502955453371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC9730.13529316067349018No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC9600.1334855439327344No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9600.1334855439327344No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9490.13195602207517185No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA9410.13084364254239902No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG9390.13056554765920586No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT9350.13000935789281945No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA9300.12931412068483647No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9240.12847983603525687No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC9030.1255598397617283No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA9020.1254207923201317No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC9010.1252817448785351No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8960.12458650767055211No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8920.12403031790416573No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA8750.12166651139702354No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG8700.12097127418904056No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA8470.1177731830323188No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC8450.1174950881491256No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8450.1174950881491256No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8320.11568747140836982No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA8290.11527032908358002No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG8200.11401890210921063No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG8170.11360175978442084No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA8050.11193319048526168No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7960.11068176351089229No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA7960.11068176351089229No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA7830.10887414677013652No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT7800.10845700444534671No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7680.10678843514618754No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT7430.10331224910627257No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7360.10233891701509638No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT7350.10219986957349979No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG7270.101087490040727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA251.233166E-437.032
TTGAAAG200.001841478237.02
TGCCGGC200.001841478237.035
GATTACG1500.035.7666661
ATTACGG1550.034.6129042
TTACGGG1550.034.6129043
ACTCAGA1150.033.7826081
CTTATAC14450.033.1591721
AGCAACG451.0790063E-732.888892
AGGGTTG401.5989644E-632.3756
TTTGAGC401.5989644E-632.3752
CGTCTTC3100.032.22580737
TCAGAGT1150.032.1739123
TTATACA14950.032.0501672
TTGTGTT352.3870747E-531.7142853
CTCAGAG1250.031.082
TATACAC15450.030.8932023
GTTCTAA303.5981496E-430.8333341
ACGTTTA303.5981496E-430.83333426
TCACGTT303.5981496E-430.83333424
Produced by FastQC (version 0.11.5)