FastQCFastQC Report
Fri 10 Feb 2017
ERR1631145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641863
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG92031.4337950621861675No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT86181.3426541177790277No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT72141.1239158512018919TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC47130.7342688393005985No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG37280.5808093004270382No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC25570.3983716151265924RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC21000.32717262094870714No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA19480.30349155505146735No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG19440.30286836910680315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15310.23852442032022408No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC14700.229020834664095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.20409339687752684No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT12780.1991079093202132No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG12120.1888253412332538No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG11220.17480365747830923No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG10560.16452108939134985No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC10180.15860082291703992No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC9930.15470591076288867No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC8530.1328944026996415No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA8480.13211542026881126No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT8190.12759732216999578No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA8140.1268183397391655No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG7830.12198864866801794No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA7500.11684736462453826No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT7340.11435462084588145No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT7340.11435462084588145No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG7210.11232926652572277No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT7100.11061550517789621No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT6920.10781116842690729No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC6730.10485103518975233No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA6620.10313727384192577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12200.032.7540971
TTATACA13000.030.3115392
TATACAC13450.030.2602233
CGTCTTC3650.028.89041137
ACGGTAC405.9361075E-527.753
CCGTCTT12100.026.14462737
GCCGTCT12300.025.7195136
GGTATCA3350.025.4029831
GTATCAA8900.022.2415731
TCACGGT502.700301E-422.21
CACGGTA502.700301E-422.22
ATCCCGA502.700301E-422.237
CGGTACT603.722986E-521.5833344
TGCCGTC14950.021.16053835
GATGCAC450.003823995620.5555553
GCGATAC450.003823995620.55555535
ATGCCGT15600.020.51602634
CATCCCG555.1396934E-420.18181836
GATTACG555.1396934E-420.1818181
CGAACTA759.2553255E-619.73333229
Produced by FastQC (version 0.11.5)