##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631145.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641863
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.692735054053593	31.0	30.0	33.0	27.0	34.0
2	30.673212196372123	31.0	30.0	34.0	27.0	34.0
3	30.098256169930345	31.0	30.0	34.0	25.0	34.0
4	34.381617261004294	35.0	35.0	37.0	30.0	37.0
5	34.34361538209867	35.0	35.0	37.0	30.0	37.0
6	34.57470363613419	35.0	35.0	37.0	31.0	37.0
7	34.620070014940886	35.0	35.0	37.0	31.0	37.0
8	34.65665881971698	35.0	35.0	37.0	32.0	37.0
9	36.34478541370978	38.0	35.0	39.0	32.0	39.0
10	36.01513407066617	37.0	35.0	39.0	31.0	39.0
11	36.16787382977364	37.0	35.0	39.0	32.0	39.0
12	36.044129354706534	37.0	35.0	39.0	32.0	39.0
13	36.177403900832424	37.0	35.0	39.0	32.0	39.0
14	37.13441809233434	38.0	36.0	40.0	32.0	41.0
15	37.12110839852118	38.0	36.0	40.0	32.0	41.0
16	37.125835263911455	38.0	36.0	40.0	32.0	41.0
17	37.03024165592969	38.0	36.0	40.0	31.0	41.0
18	37.07861802284911	38.0	36.0	40.0	32.0	41.0
19	37.120341879809246	39.0	36.0	40.0	31.0	41.0
20	37.10577646631758	39.0	36.0	40.0	31.0	41.0
21	37.07171312258223	39.0	36.0	40.0	31.0	41.0
22	36.95685372112117	39.0	36.0	40.0	31.0	41.0
23	36.95102693253856	39.0	36.0	40.0	31.0	41.0
24	36.8989909061591	39.0	36.0	40.0	31.0	41.0
25	36.83689198473817	38.0	36.0	40.0	31.0	41.0
26	36.67245502544936	38.0	36.0	40.0	30.0	41.0
27	36.57833369426186	38.0	35.0	40.0	30.0	41.0
28	36.55787761562826	38.0	35.0	40.0	30.0	41.0
29	36.46128846809989	38.0	35.0	40.0	30.0	41.0
30	36.36009086051073	38.0	35.0	40.0	30.0	41.0
31	36.31974424448831	38.0	35.0	40.0	30.0	41.0
32	36.221983507383975	38.0	35.0	40.0	30.0	41.0
33	36.136343736903356	38.0	35.0	40.0	30.0	41.0
34	36.02997991783293	38.0	35.0	40.0	30.0	41.0
35	36.02730489216546	38.0	35.0	40.0	30.0	41.0
36	36.02020057239629	38.0	35.0	40.0	30.0	41.0
37	35.921238644383614	38.0	34.0	40.0	29.0	41.0
38	35.88917572753064	38.0	34.0	40.0	29.0	41.0
39	35.78667254538741	38.0	34.0	40.0	29.0	41.0
40	35.68162209069536	38.0	34.0	40.0	28.0	41.0
41	35.62638133059547	38.0	34.0	40.0	28.0	41.0
42	35.5568478008547	38.0	34.0	40.0	28.0	41.0
43	34.742373372510954	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	8.0
11	18.0
12	9.0
13	10.0
14	12.0
15	14.0
16	19.0
17	21.0
18	36.0
19	82.0
20	165.0
21	309.0
22	567.0
23	1007.0
24	1814.0
25	2907.0
26	4477.0
27	6429.0
28	9501.0
29	12946.0
30	17437.0
31	22638.0
32	29241.0
33	38113.0
34	49863.0
35	66446.0
36	89173.0
37	115592.0
38	127330.0
39	45668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.937182856777845	22.625700499950923	14.52630857363643	22.910808069634797
2	16.091907463119075	22.612613595112975	38.7947272237222	22.50075171804575
3	16.80857129948291	27.855009558114425	32.28492684575992	23.051492296642742
4	12.44440012899949	19.509926573116072	36.99964011011696	31.046033187767485
5	13.008695001892928	37.6974525716547	35.71057998357905	13.583272442873323
6	29.103406801762993	39.428818922418024	17.142287372850593	14.32548690296839
7	26.848720053967902	32.12289850014723	22.077608461618755	18.950772984266113
8	25.40526560963944	34.88252165960649	21.491502080662073	18.220710650092
9	24.146585797903914	14.867814471312412	19.33714826995792	41.648451460825754
10	14.625395138838037	28.705346779608732	33.808304887491566	22.86095319406166
11	32.43324510059	23.378820714077612	23.94997063236236	20.237963552970026
12	21.41002051839723	25.562152671208654	29.447561239703802	23.58026557069032
13	29.098888703664176	20.63353083134563	26.417475380260274	23.85010508472992
14	22.31769084680064	21.918540249243218	25.634598037275868	30.129170866680273
15	23.89544186220424	29.23287368176698	23.002260607014268	23.86942384901451
16	25.82685090120477	27.379051292877136	22.756569548330408	24.037528257587677
17	22.919221079887762	29.31902913861681	23.966640856382128	23.795108925113304
18	23.757873564919617	25.306334840924	26.1745886583274	24.761202935828987
19	26.12442218978193	26.850745408288063	25.020448288809295	22.00438411312071
20	23.29391162911712	26.49256928659231	25.263802400200664	24.949716684089907
21	24.391653670643112	26.035462396181114	25.7615721735012	23.811311759674574
22	23.045416233682264	28.484271565739107	25.195407742773767	23.27490445780486
23	23.122691290820626	26.740597292568662	26.588228329098268	23.548483087512444
24	24.286335245994863	28.0382262258457	25.651112464809465	22.024326063349967
25	23.254027728658606	28.172678593407003	25.613877104615785	22.959416573318606
26	26.283334605671303	26.862741737722846	25.303218911200677	21.550704745405174
27	22.38141160964256	27.962353337082835	24.572221798109563	25.084013255165043
28	22.289491682804584	25.742876595161274	28.799292060766863	23.16833966126728
29	24.65806566198706	26.088433201477574	26.215563134189075	23.037938002346294
30	23.301234063966923	27.019473002805896	26.092172317145558	23.587120616081624
31	22.454947551112934	25.170636101473363	28.62043769464824	23.753978652765465
32	24.062611491860412	26.472004150418392	26.08890059093607	23.376483766785125
33	22.190405117602978	27.97310329462829	27.71681807488514	22.11967351288359
34	23.523244056753544	27.283236453885017	27.426725017643953	21.766794471717485
35	24.163100225437514	25.97703871386885	28.205551651988042	21.654309408705597
36	23.691971651271377	26.757579109560766	24.413153585734026	25.13729565343383
37	22.923583381500414	26.553797305655568	27.534691982557025	22.987927330286993
38	22.8089171676822	24.44228752864708	27.084595934023305	25.664199369647417
39	24.867144546421898	25.345595555437843	26.440689056699014	23.346570841441242
40	22.758750699136733	25.092114672445675	29.833157542964777	22.315977085452815
41	23.493642724381996	26.178483570481553	25.377222242129548	24.950651463006903
42	22.64361709586002	26.859937400971855	27.917483949067012	22.57896155410111
43	24.837387417564184	23.96212275828331	27.334337701347483	23.866152122805023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	10.5
2	16.0
3	50.5
4	85.0
5	85.0
6	129.0
7	173.0
8	169.0
9	165.0
10	251.5
11	338.0
12	338.0
13	520.0
14	702.0
15	1491.5
16	2281.0
17	2732.0
18	3183.0
19	3183.0
20	3225.5
21	3268.0
22	4221.5
23	5175.0
24	6739.0
25	8303.0
26	8303.0
27	10127.5
28	11952.0
29	14207.5
30	16463.0
31	19677.5
32	22892.0
33	22892.0
34	30444.5
35	37997.0
36	39354.0
37	40711.0
38	41277.0
39	41843.0
40	41843.0
41	42302.0
42	42761.0
43	44274.5
44	45788.0
45	45189.5
46	44591.0
47	44591.0
48	43181.5
49	41772.0
50	51238.5
51	60705.0
52	51974.5
53	43244.0
54	43244.0
55	48441.0
56	53638.0
57	43885.5
58	34133.0
59	28786.0
60	23439.0
61	23439.0
62	21266.0
63	19093.0
64	14949.5
65	10806.0
66	9145.0
67	7484.0
68	7484.0
69	6539.0
70	5594.0
71	5963.0
72	6332.0
73	5718.0
74	5104.0
75	5104.0
76	3026.0
77	948.0
78	697.0
79	446.0
80	336.5
81	227.0
82	227.0
83	170.5
84	114.0
85	89.0
86	64.0
87	42.0
88	20.0
89	20.0
90	13.0
91	6.0
92	3.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	641863.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.985245689872585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.72024610236727	45.41908300330273
2	8.487485185658352	8.994229757025234
3	2.1531303488520344	3.4225242160873837
4	0.9309379996402763	1.9730391453191425
5	0.571692770400412	1.5145640949394876
6	0.34859774776932007	1.1082322387496197
7	0.24153851951733565	0.8958584460135881
8	0.18014016161770036	0.7635816577541767
9	0.14177504546039227	0.6760787054770565
>10	1.0065799273175327	10.500132669793661
>50	0.11736343282016486	4.320547102466259
>100	0.08632272207388766	9.067619239247202
>500	0.00916439857633731	3.1958858390775426
>1k	0.00413876064737814	4.227190665058355
>5k	8.868772815810301E-4	3.9214332196885886
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	9203	1.4337950621861675	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	8618	1.3426541177790277	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	7214	1.1239158512018919	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	4713	0.7342688393005985	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	3728	0.5808093004270382	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	2557	0.3983716151265924	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	2100	0.32717262094870714	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1948	0.30349155505146735	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1944	0.30286836910680315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1531	0.23852442032022408	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1470	0.229020834664095	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1310	0.20409339687752684	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1278	0.1991079093202132	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1212	0.1888253412332538	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1122	0.17480365747830923	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	1056	0.16452108939134985	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	1018	0.15860082291703992	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	993	0.15470591076288867	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	853	0.1328944026996415	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	848	0.13211542026881126	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	819	0.12759732216999578	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	814	0.1268183397391655	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	783	0.12198864866801794	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	750	0.11684736462453826	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	734	0.11435462084588145	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	734	0.11435462084588145	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	721	0.11232926652572277	No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT	710	0.11061550517789621	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	692	0.10781116842690729	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	673	0.10485103518975233	No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA	662	0.10313727384192577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.55796486166051E-4	0.0
18	0.0	0.0	0.0	3.11592972332102E-4	0.0
19	0.0	0.0	0.0	3.11592972332102E-4	0.0
20	0.0	0.0	0.0	4.6738945849815304E-4	0.0
21	0.0	0.0	0.0	6.23185944664204E-4	0.0
22	0.0	0.0	0.0	0.0010905754031623571	0.0
23	0.0	0.0	0.0	0.00155796486166051	0.0
24	0.0	0.0	0.0	0.002025354320158663	0.0
25	0.0	0.0	0.0	0.0023369472924907654	0.0
26	0.0	0.0	0.0	0.0023369472924907654	0.0
27	0.0	0.0	0.0	0.002492743778656816	0.0
28	1.55796486166051E-4	0.0	0.0	0.006543452418974143	0.0
29	1.55796486166051E-4	0.0	0.0	0.015268055644272999	0.0
30	1.55796486166051E-4	0.0	0.0	0.025862216703564467	0.0
31	1.55796486166051E-4	0.0	0.0	0.05359399124112155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1220	0.0	32.754097	1
TTATACA	1300	0.0	30.311539	2
TATACAC	1345	0.0	30.260223	3
CGTCTTC	365	0.0	28.890411	37
ACGGTAC	40	5.9361075E-5	27.75	3
CCGTCTT	1210	0.0	26.144627	37
GCCGTCT	1230	0.0	25.71951	36
GGTATCA	335	0.0	25.402983	1
GTATCAA	890	0.0	22.241573	1
TCACGGT	50	2.700301E-4	22.2	1
CACGGTA	50	2.700301E-4	22.2	2
ATCCCGA	50	2.700301E-4	22.2	37
CGGTACT	60	3.722986E-5	21.583334	4
TGCCGTC	1495	0.0	21.160538	35
GATGCAC	45	0.0038239956	20.555555	3
GCGATAC	45	0.0038239956	20.555555	35
ATGCCGT	1560	0.0	20.516026	34
CATCCCG	55	5.1396934E-4	20.181818	36
GATTACG	55	5.1396934E-4	20.181818	1
CGAACTA	75	9.2553255E-6	19.733332	29
>>END_MODULE