FastQCFastQC Report
Fri 10 Feb 2017
ERR1631144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831582
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT162521.9543472561936164No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG132181.5895004942386919No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT99211.193027266102441TruSeq Adapter, Index 6 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95011.1425211223908165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT92321.1101731398707524No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC76380.9184902992128257No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA41630.5006120863606957No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC37490.4508274589878088No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC37200.44734013001724426RNA PCR Primer, Index 6 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32670.39286564644256367No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA25940.31193556378084186No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT22130.2661192762710111No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG18630.22403082317799086No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18170.21849919791433678No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT18150.21825869246809093No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT18110.2177776815755993No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC16690.20070179489214535No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG16510.19853724587593288No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14690.17665125026756232No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG14470.17400569035885818No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA12540.15079691479613558No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12290.1477905967180627No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11890.1429804877931461No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.13780962069886074No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT11120.1337210281126816No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10810.12999319369587126No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT10790.1297526882496254No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10150.12205651396975883No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10090.12133499763102135No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10040.12073373401540678No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9500.11424008696676935No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9490.11411983424364645No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9310.11195528522743396No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9050.10882871442623818No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8850.10642365996377987No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8670.1042591109475674No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8390.10089203470012578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAG451.0793519E-732.888892
CTTATAC20000.032.8374981
GATTACG1700.031.5588251
TTATACA20950.031.348452
TATACAC21800.030.2958743
GGTATCA11750.029.9148941
CTCGTCG250.005495480329.69
TTACGGG1950.028.461543
CGTCTTC4100.027.97560937
AGCAACG604.319736E-827.7499982
TCGCCGA556.258251E-726.909091
CCGTCTT14200.026.44718237
ATTACGG2050.026.170732
GCCGTCT14850.025.16498436
GACCGTT901.4188117E-1024.6666667
GTATCAA36500.023.8726021
GCAACGC701.9215986E-723.7857153
TCAGAGT1350.023.2962973
CGAGAGA400.001930740523.12532
GTTATGC1054.0017767E-1122.904761
Produced by FastQC (version 0.11.5)