##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631144.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831582
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35094073705299	31.0	31.0	34.0	28.0	34.0
2	31.31168904569844	31.0	31.0	34.0	28.0	34.0
3	30.75839183628313	31.0	31.0	34.0	26.0	34.0
4	34.96058356241477	37.0	35.0	37.0	32.0	37.0
5	35.17760966447085	37.0	35.0	37.0	32.0	37.0
6	35.39603671075132	37.0	35.0	37.0	33.0	37.0
7	35.40873780336756	37.0	35.0	37.0	33.0	37.0
8	35.420604342085326	37.0	35.0	37.0	33.0	37.0
9	37.197773641084105	39.0	37.0	39.0	34.0	39.0
10	37.024828579743186	39.0	37.0	39.0	33.0	39.0
11	37.08078096928505	39.0	37.0	39.0	33.0	39.0
12	37.004400047139065	39.0	37.0	39.0	33.0	39.0
13	37.07487415552525	39.0	37.0	39.0	33.0	39.0
14	38.089982707658415	40.0	37.0	41.0	33.0	41.0
15	38.0841408303691	40.0	37.0	41.0	33.0	41.0
16	38.113232369147	40.0	37.0	41.0	33.0	41.0
17	38.13755588745307	40.0	37.0	41.0	33.0	41.0
18	38.199946607790935	40.0	38.0	41.0	33.0	41.0
19	38.21525117186279	40.0	38.0	41.0	34.0	41.0
20	38.20297817894086	40.0	38.0	41.0	34.0	41.0
21	38.12564365270052	40.0	37.0	41.0	33.0	41.0
22	38.05496391215779	40.0	37.0	41.0	33.0	41.0
23	38.029844320824644	40.0	37.0	41.0	33.0	41.0
24	37.9807391213374	40.0	37.0	41.0	33.0	41.0
25	37.92632596665152	40.0	37.0	41.0	33.0	41.0
26	37.756957221296275	40.0	37.0	41.0	33.0	41.0
27	37.6988787636096	40.0	37.0	41.0	33.0	41.0
28	37.63585190636642	40.0	37.0	41.0	32.0	41.0
29	37.54953450170879	39.0	37.0	41.0	32.0	41.0
30	37.390880273983804	39.0	36.0	41.0	32.0	41.0
31	37.23185326281714	39.0	36.0	41.0	32.0	41.0
32	37.130938380099614	39.0	36.0	41.0	31.0	41.0
33	37.032513931277975	39.0	35.0	40.0	31.0	41.0
34	36.96299462951338	39.0	35.0	40.0	31.0	41.0
35	36.90142283021999	39.0	35.0	40.0	31.0	41.0
36	36.76252371984964	39.0	35.0	40.0	31.0	41.0
37	36.70282786303696	39.0	35.0	40.0	30.0	41.0
38	36.62451207457593	39.0	35.0	40.0	30.0	41.0
39	36.49195749787754	39.0	35.0	40.0	30.0	41.0
40	36.330310179874026	39.0	35.0	40.0	30.0	41.0
41	36.216472939529716	39.0	35.0	40.0	30.0	41.0
42	36.056746057514474	39.0	35.0	40.0	29.0	41.0
43	35.27354367939662	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	8.0
11	10.0
12	6.0
13	13.0
14	3.0
15	8.0
16	8.0
17	15.0
18	31.0
19	53.0
20	130.0
21	259.0
22	509.0
23	959.0
24	1623.0
25	2683.0
26	4186.0
27	6410.0
28	9046.0
29	12509.0
30	16530.0
31	21316.0
32	27342.0
33	35048.0
34	46333.0
35	61735.0
36	84218.0
37	125169.0
38	195245.0
39	180169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.98222664752243	21.206206964556714	15.898973282249976	22.91259310567088
2	14.44451659607832	23.08623803786037	40.53346513031787	21.935780235743437
3	15.152564629826044	29.823637356267934	33.363156008667815	21.66064200523821
4	11.20719303688632	18.88208258476013	37.4379195316878	32.47280484666575
5	13.189078166675083	38.13213850227638	33.17387822247235	15.504905108576184
6	26.996014824755704	40.26926989761683	16.56781892825963	16.166896349367832
7	24.880528919577383	32.18948943098817	21.33066853298893	21.599313116445522
8	26.233251801987056	32.11757830256066	22.117482100382162	19.53168779507012
9	24.81475068002915	13.352020606506635	18.943291220829693	42.88993749263452
10	16.02583990514465	27.931701263375107	31.25572703593873	24.78673179554151
11	32.4845896135318	23.247496939568197	23.678001688348232	20.58991175855177
12	22.188551459747806	27.25804550844054	26.806256027667747	23.74714700414391
13	31.293486390999327	20.663867183272366	25.577152944628427	22.46549348109988
14	22.76756832158464	22.047014004632135	27.56336717244962	27.6220505013336
15	25.21567325892095	27.27908973498705	23.74221664249587	23.763020363596134
16	23.802343004057327	25.842670957283826	23.33588269106354	27.01910334759531
17	21.353877308551652	28.51264216878191	24.508948005127575	25.62453251753886
18	24.89063014831971	22.981137157850938	26.852312820623826	25.275919873205527
19	25.96520848214608	24.401562323378815	27.167495207928983	22.465733986546123
20	25.103236962801024	23.682210533657535	27.164729395297154	24.049823108244286
21	24.792984937143903	25.01689550759877	27.658968087332337	22.531151467924992
22	21.626369979148176	28.386857820395345	28.57072423405028	21.4160479664062
23	22.956966360503234	24.41454961747609	27.81818269274708	24.810301329273603
24	23.472249279084924	26.837882493849076	27.1723053168539	22.517562910212103
25	23.62881832459096	26.94586943921345	27.760100627478707	21.66521160871688
26	23.500989679911303	26.09520167584195	28.33021878780445	22.0735898564423
27	23.171978229447003	27.142362388796293	28.71839457804522	20.96726480371148
28	20.110945162353204	26.418080237426977	29.470334855732812	24.000639744487014
29	22.297861185066537	25.323179193392836	28.459129706992215	23.919829914548416
30	23.851285862368353	24.498125260046514	29.27865201507488	22.371936862510253
31	23.495097296478278	27.0180210730872	27.471614344706836	22.015267285727685
32	22.402481054183472	24.495599952860932	30.889677746752575	22.21224124620302
33	20.520886695479216	24.244873025149655	31.71244687835956	23.521793401011564
34	20.735898564422993	27.954308775322218	28.478249889968758	22.831542770286035
35	23.149250464776774	24.915642714729273	31.472903453898716	20.462203366595237
36	23.090206377723423	24.87295299802064	28.63048983744237	23.40635078681357
37	22.622663790221527	25.95053764992508	28.683521288339577	22.74327727151381
38	21.832964157473345	24.2293604238668	29.184373880146513	24.75330153851334
39	23.608736119829434	24.100690010125277	28.44974999458863	23.84082387545666
40	20.93756238110012	24.65625759095315	32.76958856733311	21.636591460613623
41	23.166326351460228	24.143018968664546	27.23676077644778	25.453893903427442
42	22.65777758537342	24.206392153750322	30.399166889134204	22.736663371742054
43	23.365464860951775	23.34502189802088	30.08566803995276	23.20384520107458
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	36.5
2	45.0
3	199.5
4	354.0
5	354.0
6	513.0
7	672.0
8	730.5
9	789.0
10	1204.0
11	1619.0
12	1619.0
13	2530.0
14	3441.0
15	8602.5
16	13764.0
17	14290.0
18	14816.0
19	14816.0
20	12027.0
21	9238.0
22	7889.0
23	6540.0
24	7622.0
25	8704.0
26	8704.0
27	10002.0
28	11300.0
29	13381.0
30	15462.0
31	18123.0
32	20784.0
33	20784.0
34	25079.5
35	29375.0
36	32550.5
37	35726.0
38	39496.5
39	43267.0
40	43267.0
41	46300.0
42	49333.0
43	52910.0
44	56487.0
45	60961.5
46	65436.0
47	65436.0
48	70633.5
49	75831.0
50	86641.5
51	97452.0
52	82098.5
53	66745.0
54	66745.0
55	73174.5
56	79604.0
57	66963.5
58	54323.0
59	41457.0
60	28591.0
61	28591.0
62	23696.5
63	18802.0
64	14704.0
65	10606.0
66	8405.0
67	6204.0
68	6204.0
69	4886.0
70	3568.0
71	2554.0
72	1540.0
73	1044.0
74	548.0
75	548.0
76	398.0
77	248.0
78	192.0
79	136.0
80	103.5
81	71.0
82	71.0
83	57.5
84	44.0
85	39.0
86	34.0
87	33.5
88	33.0
89	33.0
90	27.0
91	21.0
92	11.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	831582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.1096038443779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8610476537581	21.209562328872384
2	13.074217311637753	7.611705730341649
3	4.709211726518987	4.1124986333479985
4	2.247027305204991	2.616402987280693
5	1.245437583798882	1.812709733864233
6	0.8438096378892337	1.4737778567414135
7	0.598220961770329	1.218978264597591
8	0.4575453480011471	1.0655171056921133
9	0.36693357294404955	0.9613161851043082
>10	2.8564669808163976	17.203959219433006
>50	0.41572502790907256	8.478488204283712
>100	0.2961158289276735	15.816936423818454
>500	0.015781769283662823	3.104221265091005
>1k	0.009967433231787047	5.363628174904682
>5k	0.0016612388719645078	4.387521834820375
>10k+	8.306194359822539E-4	3.562776051806361
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	16252	1.9543472561936164	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	13218	1.5895004942386919	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	9921	1.193027266102441	TruSeq Adapter, Index 6 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9501	1.1425211223908165	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9232	1.1101731398707524	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	7638	0.9184902992128257	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	4163	0.5006120863606957	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	3749	0.4508274589878088	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	3720	0.44734013001724426	RNA PCR Primer, Index 6 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3267	0.39286564644256367	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	2594	0.31193556378084186	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	2213	0.2661192762710111	No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG	1863	0.22403082317799086	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1817	0.21849919791433678	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1815	0.21825869246809093	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1811	0.2177776815755993	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1669	0.20070179489214535	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1651	0.19853724587593288	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	1469	0.17665125026756232	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	1447	0.17400569035885818	No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA	1254	0.15079691479613558	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	1229	0.1477905967180627	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	1189	0.1429804877931461	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1146	0.13780962069886074	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	1112	0.1337210281126816	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	1081	0.12999319369587126	No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT	1079	0.1297526882496254	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	1015	0.12205651396975883	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	1009	0.12133499763102135	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	1004	0.12073373401540678	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	950	0.11424008696676935	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	949	0.11411983424364645	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	931	0.11195528522743396	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	905	0.10882871442623818	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	885	0.10642365996377987	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	867	0.1042591109475674	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	839	0.10089203470012578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2025272312291512E-4	0.0
2	0.0	0.0	0.0	1.2025272312291512E-4	0.0
3	0.0	0.0	0.0	1.2025272312291512E-4	0.0
4	0.0	0.0	0.0	1.2025272312291512E-4	0.0
5	0.0	0.0	0.0	1.2025272312291512E-4	0.0
6	0.0	0.0	0.0	1.2025272312291512E-4	0.0
7	0.0	0.0	0.0	1.2025272312291512E-4	0.0
8	0.0	0.0	0.0	2.4050544624583023E-4	0.0
9	0.0	0.0	0.0	2.4050544624583023E-4	0.0
10	0.0	0.0	0.0	2.4050544624583023E-4	0.0
11	0.0	0.0	0.0	2.4050544624583023E-4	0.0
12	0.0	0.0	0.0	2.4050544624583023E-4	0.0
13	0.0	0.0	0.0	2.4050544624583023E-4	0.0
14	0.0	0.0	0.0	2.4050544624583023E-4	0.0
15	0.0	0.0	0.0	2.4050544624583023E-4	0.0
16	0.0	0.0	0.0	6.012636156145756E-4	0.0
17	0.0	0.0	0.0	6.012636156145756E-4	0.0
18	0.0	0.0	0.0	7.215163387374907E-4	0.0
19	0.0	0.0	0.0	7.215163387374907E-4	0.0
20	0.0	0.0	0.0	9.620217849833209E-4	0.0
21	0.0	0.0	0.0	0.0013227799543520664	0.0
22	0.0	0.0	0.0	0.0021645490162124723	0.0
23	0.0	0.0	0.0	0.003006318078072878	0.0
24	0.0	0.0	0.0	0.004569603478670774	0.0
25	0.0	0.0	0.0	0.005050614371162435	0.0
26	0.0	0.0	0.0	0.006132888879268671	0.0
27	0.0	0.0	0.0	0.006974657941129077	0.0
28	0.0	0.0	0.0	0.022246753777739298	0.0
29	0.0	0.0	0.0	0.05615802169840136	0.0
30	0.0	0.0	0.0	0.09451864037461129	0.0
31	0.0	0.0	0.0	0.16426521978590206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGAG	45	1.0793519E-7	32.88889	2
CTTATAC	2000	0.0	32.837498	1
GATTACG	170	0.0	31.558825	1
TTATACA	2095	0.0	31.34845	2
TATACAC	2180	0.0	30.295874	3
GGTATCA	1175	0.0	29.914894	1
CTCGTCG	25	0.0054954803	29.6	9
TTACGGG	195	0.0	28.46154	3
CGTCTTC	410	0.0	27.975609	37
AGCAACG	60	4.319736E-8	27.749998	2
TCGCCGA	55	6.258251E-7	26.90909	1
CCGTCTT	1420	0.0	26.447182	37
ATTACGG	205	0.0	26.17073	2
GCCGTCT	1485	0.0	25.164984	36
GACCGTT	90	1.4188117E-10	24.666666	7
GTATCAA	3650	0.0	23.872602	1
GCAACGC	70	1.9215986E-7	23.785715	3
TCAGAGT	135	0.0	23.296297	3
CGAGAGA	40	0.0019307405	23.125	32
GTTATGC	105	4.0017767E-11	22.90476	1
>>END_MODULE