FastQCFastQC Report
Fri 10 Feb 2017
ERR1631143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429346
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT253505.904328909550805No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG192254.477740563554802No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT143383.3394977477372563Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC100682.3449618722428998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67871.5807763435550812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66191.5416470632077623No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC64131.4936671123056928Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC46051.0725615238059747No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA46031.072095699039935No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA25640.5971873500626534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25420.5920632776362188No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT24540.5715669879304803No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG22710.528944021837865No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20160.46955136416782733No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19810.46139943076213585No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA19540.45511079642060254No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19200.44719177539793087No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC18750.43671071816204177No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG18280.4257638361601133No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC16820.39175862823922897No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG16260.37871553479012265No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT15010.3496014869126532No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14010.32631024861067764No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12940.3013886236275638No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12010.27972777200672655No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11810.27506952434633136No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11390.26528720425950164No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT11340.2641226423444029No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10940.25480614702361265No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10680.248750425065099No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG10450.24339344025564466No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG10290.23966684212732856No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10240.23850228021222974No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10010.2331452954027754No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9770.22755539821030127No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9480.22080093910272833No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG8960.20868949518570104No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT8750.20379833514228618No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8740.20356542275926642No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8280.19285145314035768No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8210.19122106645921938No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8090.1884261178629823No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7980.18586408164976498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.18004127207427112No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7670.17864379777615258No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7360.17142351390254013No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6970.16233993096476967No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6830.15907915760249308No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC6670.155352559474177No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG6560.15279052326095968No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6450.1502284870477424No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6310.1469677136854658No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6250.14557023938734726No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6160.14347402794016945No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA6120.14254237840809045No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG6020.14021325457789288No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA5930.1381170431307151No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5760.13415753261937924No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5690.13252714593824094No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.1322942335552212No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5590.1301980221080434No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5520.1285676354269051No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5460.12717016112878657No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5450.1269372487457668No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC5440.12670433636274706No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5410.1260055992136878No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5200.12111443917027293No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5170.12041570202121366No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4970.11575745436081855No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4960.11552454197779878No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4950.11529162959477902No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4920.11459289244571977No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4920.11459289244571977No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT4870.11342833053062099No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4850.11296250576458147No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4830.11249668099854197No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4750.11063338193438392No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4750.11063338193438392No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4630.10783843333814687No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA4570.10644095904002833No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4480.10434474759285052No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA4480.10434474759285052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC4380.10201562376265297No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG4300.10015232469849493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG308.299849E-637.01
TCGCCGA452.6120688E-937.01
ATTACGG800.037.02
TTACGGG800.037.03
ACTCAGA501.7826096E-1037.01
GATTACG800.037.01
CTTATAC27950.035.610021
TTTACAC950.035.052632
CGTCTTC7100.034.91549337
TTATACA29350.033.9114152
GATTAGA451.0772965E-732.888892
TATACAC31400.031.933123
TTAGGAC658.731149E-1131.307693
AGCAACG658.731149E-1131.307692
TAGGACC658.731149E-1131.307694
CTTAGGA658.731149E-1131.307692
TCAGAGT601.2660166E-930.8333343
CGCCGAG551.8500941E-830.2727282
TGGAGCA250.00549255629.65
TTGTGTG250.00549255629.63
Produced by FastQC (version 0.11.5)