FastQCFastQC Report
Fri 10 Feb 2017
ERR1631142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068986
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT192961.805075089851504No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG157731.475510436993562No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT135941.2716724073093566TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC81690.7641821314778678No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC56490.5284447130271117RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC41080.3842894107125818No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG19260.18017074124450647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19100.1786739957305334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.16875805670046193No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA15980.14948745820805887No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT12520.11712033646839154No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12070.11291073971034234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC7150.031.30769337
CTTATAC25250.031.211881
TTATACA27850.029.161582
TATACAC28250.028.8141613
ACGCTAC405.9397455E-527.75000211
CCGTCTT21400.024.55140137
GCCGTCT21950.024.020536
GGTATCA5100.022.8529421
GTATCAA13050.022.540231
GGTCTAC1059.822543E-1021.1428571
GACGCTA555.14277E-420.18181810
TGCCGTC26300.020.0475335
GTTCTAG851.2451419E-619.5882361
ATGCCGT28000.018.89642734
TTGCGCG500.00703456718.518
TAGTACC500.00703456718.54
TATACTG3350.018.2238795
ATACACA45850.017.9956384
TATGCCG29650.017.90725133
TACACAT47000.017.8702135
Produced by FastQC (version 0.11.5)