##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631142.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1068986
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084094646702574	31.0	31.0	34.0	28.0	34.0
2	31.07437328458932	31.0	31.0	34.0	28.0	34.0
3	30.49964265200854	31.0	30.0	34.0	26.0	34.0
4	34.73940912228972	37.0	35.0	37.0	32.0	37.0
5	34.87291133840855	35.0	35.0	37.0	32.0	37.0
6	35.078859779267454	36.0	35.0	37.0	32.0	37.0
7	35.09015553056822	36.0	35.0	37.0	32.0	37.0
8	35.12485851077563	37.0	35.0	37.0	32.0	37.0
9	36.85151161942252	39.0	37.0	39.0	33.0	39.0
10	36.62369292020663	38.0	35.0	39.0	32.0	39.0
11	36.70085763517951	38.0	35.0	39.0	32.0	39.0
12	36.620426273122376	38.0	35.0	39.0	32.0	39.0
13	36.72008052490865	38.0	35.0	39.0	32.0	39.0
14	37.7682719885948	39.0	37.0	40.0	33.0	41.0
15	37.76712417187877	39.0	37.0	40.0	33.0	41.0
16	37.76693614322358	39.0	37.0	40.0	33.0	41.0
17	37.697806145262895	39.0	37.0	40.0	32.0	41.0
18	37.76579861663296	39.0	37.0	40.0	33.0	41.0
19	37.763567530351196	39.0	37.0	40.0	32.0	41.0
20	37.723452879644825	39.0	37.0	40.0	32.0	41.0
21	37.69831597420359	39.0	37.0	40.0	32.0	41.0
22	37.61399962207176	39.0	37.0	40.0	32.0	41.0
23	37.6158640057026	39.0	37.0	40.0	32.0	41.0
24	37.5728372495056	39.0	37.0	40.0	32.0	41.0
25	37.495914820212796	39.0	36.0	40.0	32.0	41.0
26	37.37380845025098	39.0	36.0	40.0	32.0	41.0
27	37.25169179016376	39.0	36.0	40.0	31.0	41.0
28	37.25463944336035	39.0	36.0	40.0	31.0	41.0
29	37.20836474939803	39.0	36.0	40.0	31.0	41.0
30	37.048046466464484	39.0	36.0	40.0	31.0	41.0
31	37.078704491920384	39.0	36.0	40.0	31.0	41.0
32	37.019903908938005	39.0	36.0	40.0	31.0	41.0
33	36.94241365181583	39.0	36.0	40.0	31.0	41.0
34	36.89358700675219	39.0	36.0	40.0	30.0	41.0
35	36.87202264575963	39.0	36.0	40.0	30.0	41.0
36	36.83472655394926	39.0	36.0	40.0	30.0	41.0
37	36.7884368925318	39.0	35.0	40.0	30.0	41.0
38	36.76578832650755	39.0	35.0	40.0	30.0	41.0
39	36.69858819479394	39.0	35.0	40.0	30.0	41.0
40	36.600107952770195	39.0	35.0	40.0	30.0	41.0
41	36.55341791192775	39.0	35.0	40.0	30.0	41.0
42	36.49394285799814	38.0	35.0	40.0	30.0	41.0
43	35.71995330153997	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	14.0
10	15.0
11	24.0
12	28.0
13	14.0
14	16.0
15	12.0
16	25.0
17	19.0
18	31.0
19	70.0
20	122.0
21	307.0
22	606.0
23	1113.0
24	1998.0
25	3412.0
26	5333.0
27	8248.0
28	11767.0
29	16612.0
30	22366.0
31	29674.0
32	38345.0
33	49829.0
34	65537.0
35	87265.0
36	121377.0
37	177814.0
38	269837.0
39	157153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.31958510214353	21.6594978792987	14.872785985971754	25.14813103258602
2	14.698040947215398	21.53704538693678	39.94084113356022	23.82407253228761
3	15.770646201166338	27.601390476582484	33.26675934015974	23.36120398209144
4	12.515318254869568	19.263395404617086	36.460346534005126	31.760939806508226
5	13.259013682124928	37.47364324696488	35.216644558488134	14.050698512422052
6	28.912446000228254	39.124834188661026	17.274781896114636	14.68793791499608
7	25.420538716129116	32.70061534949944	22.069512603532694	19.809333330838758
8	25.208842772496553	34.99858744642118	21.701687393473815	18.090882387608445
9	24.1658917890412	14.841728516556813	18.94664663522254	42.04573305917945
10	14.721427595871228	28.406078283532242	33.04823449511967	23.824259625476856
11	32.155706435818615	22.345662150860722	24.94466718928031	20.553964224040353
12	20.95284690351417	25.508285421885784	29.070726838330906	24.468140836269136
13	29.49917024170569	20.010177869495017	26.261990334765844	24.228661554033447
14	21.915160722404224	21.63218227366869	25.93813202417992	30.514524979747158
15	24.36823307321144	28.211688459904995	22.11029891878846	25.30977954809511
16	25.508846701453525	26.840014742943314	23.06980633983981	24.58133221576335
17	21.588402467384977	30.145577210552805	24.462902226970233	23.803118095091985
18	25.09677395213782	25.14859876555914	24.938118927656678	24.816508354646366
19	25.071984104562638	26.902971601124804	25.116512283603342	22.908532010709216
20	22.82546263468371	25.915026015307962	26.0684424304902	25.191068919518123
21	25.240087335100743	26.458531730069428	24.715478032453184	23.585902902376645
22	23.19029435371464	29.625364597852546	25.25533543002434	21.929005618408475
23	24.333901472984678	26.435612814386715	25.045042685311124	24.185443027317476
24	26.9117649810194	26.65292155369668	24.69162365082424	21.743689814459685
25	23.77393155756951	27.912619996894257	25.546639525681346	22.766808919854892
26	26.319240850675314	27.756116544089444	24.553829516944095	21.370813088291147
27	23.39619040848056	28.218798001096367	26.53673668317452	21.848274907248552
28	22.069886789911187	27.580061853008363	26.25880975054865	24.091241606531796
29	26.812792683907926	25.209404052064293	25.171891867620346	22.805911396407435
30	22.65698521776712	26.870791572574383	28.313280061665914	22.15894314799258
31	24.73175513991764	27.379591500730598	24.68095933903718	23.20769402031458
32	26.64450236018058	25.488547090420266	25.918674332498277	21.948276216900876
33	21.981298164802908	28.653976759284035	27.27865472513204	22.08607035078102
34	24.4230513776607	27.36527887175323	25.471895796577314	22.73977395400875
35	24.36720406067058	25.204913815522374	28.7862516440814	21.641630479725645
36	23.645959816124815	27.268458146318096	24.672353052331836	24.413228985225253
37	22.856333010909406	26.354788556632176	26.999605233370687	23.789273199087734
38	22.829110951874018	24.67272723871033	27.49278288022481	25.00537892919084
39	24.603689851878322	25.12886043409362	25.958805821591675	24.308643892436383
40	22.050616191418783	24.538768515209743	30.397498189873396	23.013117103498082
41	23.239967595459625	25.409313124774318	25.652160084416447	25.698559195349613
42	23.692358927057978	24.59012559565794	28.721985133575185	22.995530343708896
43	23.7674768425405	24.080670841339362	28.457996643548185	23.69385567257195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	11.0
2	15.0
3	69.5
4	124.0
5	124.0
6	135.5
7	147.0
8	178.5
9	210.0
10	315.5
11	421.0
12	421.0
13	649.0
14	877.0
15	2038.5
16	3200.0
17	3721.0
18	4242.0
19	4242.0
20	4498.0
21	4754.0
22	5987.0
23	7220.0
24	9823.0
25	12426.0
26	12426.0
27	15978.5
28	19531.0
29	23982.5
30	28434.0
31	33606.5
32	38779.0
33	38779.0
34	44491.0
35	50203.0
36	55071.5
37	59940.0
38	64146.0
39	68352.0
40	68352.0
41	70915.5
42	73479.0
43	75048.0
44	76617.0
45	77926.5
46	79236.0
47	79236.0
48	80740.5
49	82245.0
50	96722.5
51	111200.0
52	90515.0
53	69830.0
54	69830.0
55	77488.5
56	85147.0
57	74222.0
58	63297.0
59	51524.5
60	39752.0
61	39752.0
62	35434.5
63	31117.0
64	26237.0
65	21357.0
66	17866.0
67	14375.0
68	14375.0
69	12017.5
70	9660.0
71	7795.0
72	5930.0
73	4637.5
74	3345.0
75	3345.0
76	2529.0
77	1713.0
78	1314.5
79	916.0
80	688.0
81	460.0
82	460.0
83	352.0
84	244.0
85	181.0
86	118.0
87	81.0
88	44.0
89	44.0
90	31.0
91	18.0
92	11.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1068986.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.87274138026602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91908835120597	46.251367314959744
2	12.073003520556814	13.973956208564514
3	3.4551147801017237	5.998708923238853
4	1.5097151881000535	3.4948542655509627
5	0.7958013537364842	2.302760296742857
6	0.48906492134856494	1.6982116626819386
7	0.32372996392033193	1.3114598335302956
8	0.22959421993899365	1.0629797530346639
9	0.16534572367892159	0.8612109274322843
>10	0.9440460527193665	10.137893527684252
>50	0.0658842134717941	2.606728421814357
>100	0.026213646764052374	2.5932283780153913
>500	0.0012945007454951039	0.4646683933028698
>1k	0.0012945007454951039	1.3909148822506097
>5k	3.2362518637377597E-4	1.2939919102497086
>10k+	4.85437779560664E-4	4.5570653009467055
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	19296	1.805075089851504	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	15773	1.475510436993562	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	13594	1.2716724073093566	TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	8169	0.7641821314778678	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	5649	0.5284447130271117	RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	4108	0.3842894107125818	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	1926	0.18017074124450647	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1910	0.1786739957305334	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1804	0.16875805670046193	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1598	0.14948745820805887	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	1252	0.11712033646839154	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1207	0.11291073971034234	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.354659462331593E-5	0.0
9	0.0	0.0	0.0	9.354659462331593E-5	0.0
10	0.0	0.0	0.0	1.8709318924663187E-4	0.0
11	0.0	0.0	0.0	1.8709318924663187E-4	0.0
12	0.0	0.0	0.0	1.8709318924663187E-4	0.0
13	0.0	0.0	0.0	2.806397838699478E-4	0.0
14	0.0	0.0	0.0	2.806397838699478E-4	0.0
15	0.0	0.0	0.0	2.806397838699478E-4	0.0
16	0.0	0.0	0.0	2.806397838699478E-4	0.0
17	0.0	0.0	0.0	3.7418637849326373E-4	0.0
18	0.0	0.0	0.0	4.677329731165796E-4	0.0
19	0.0	0.0	0.0	5.612795677398956E-4	0.0
20	0.0	0.0	0.0	6.548261623632115E-4	0.0
21	0.0	0.0	0.0	6.548261623632115E-4	0.0
22	0.0	0.0	0.0	6.548261623632115E-4	0.0
23	0.0	0.0	0.0	9.354659462331592E-4	0.0
24	0.0	0.0	0.0	0.001309652324726423	0.0
25	0.0	0.0	0.0	0.001496745513973055	0.0
26	0.0	0.0	0.0	0.001496745513973055	0.0
27	0.0	0.0	0.0	0.002432211460206214	0.0
28	0.0	0.0	0.0	0.007109541191372011	0.0
29	0.0	0.0	0.0	0.015154548328977181	0.0
30	0.0	0.0	0.0	0.031618748982680786	0.0
31	0.0	0.0	0.0	0.07942105883519522	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	715	0.0	31.307693	37
CTTATAC	2525	0.0	31.21188	1
TTATACA	2785	0.0	29.16158	2
TATACAC	2825	0.0	28.814161	3
ACGCTAC	40	5.9397455E-5	27.750002	11
CCGTCTT	2140	0.0	24.551401	37
GCCGTCT	2195	0.0	24.0205	36
GGTATCA	510	0.0	22.852942	1
GTATCAA	1305	0.0	22.54023	1
GGTCTAC	105	9.822543E-10	21.142857	1
GACGCTA	55	5.14277E-4	20.181818	10
TGCCGTC	2630	0.0	20.04753	35
GTTCTAG	85	1.2451419E-6	19.588236	1
ATGCCGT	2800	0.0	18.896427	34
TTGCGCG	50	0.007034567	18.5	18
TAGTACC	50	0.007034567	18.5	4
TATACTG	335	0.0	18.223879	5
ATACACA	4585	0.0	17.995638	4
TATGCCG	2965	0.0	17.907251	33
TACACAT	4700	0.0	17.870213	5
>>END_MODULE