FastQCFastQC Report
Fri 10 Feb 2017
ERR1631141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239882
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT149601.2065664313216902No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG111250.8972628040410296No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT87250.7036959968771221RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC61560.4964988603754228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41150.33188642144978314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.29204392030854553No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC29490.23784521430265138RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC27990.22574728885490716No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18250.14719142628088802No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT16560.1335610969430962No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG15790.1273508285465875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13620.10984916306551752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19200.031.1223951
TATACAC21650.028.2840653
TTATACA21700.027.9631352
GGTATCA8200.026.396341
CGTCTTC4700.026.37234137
CCGTCTT13900.025.15467637
GCCGTCT14150.024.71024736
GTATCAA22900.023.9934481
TTACGGG903.8289727E-922.6111133
TGCCGTC17300.020.10404835
ATTACGG951.6763806E-719.4736842
GATTACG951.6763806E-719.4736841
ATGCCGT18350.018.85286134
ATACACA34350.018.257644
TACACAT33750.018.2533345
TATGCCG19500.017.64615433
GTCTTAT1054.798021E-717.6190471
ATTAGAC1005.8785427E-616.653
AACGGCC904.446409E-516.44444537
GCGACCC803.382833E-416.187521