##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631141.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239882
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519727683763456	31.0	31.0	34.0	28.0	34.0
2	31.508117707975437	31.0	31.0	34.0	28.0	34.0
3	30.97723251083571	31.0	31.0	34.0	27.0	34.0
4	35.13138750300432	37.0	35.0	37.0	32.0	37.0
5	35.331367823712256	37.0	35.0	37.0	33.0	37.0
6	35.51731939007099	37.0	35.0	37.0	33.0	37.0
7	35.53285231981753	37.0	35.0	37.0	33.0	37.0
8	35.544493750211714	37.0	35.0	37.0	33.0	37.0
9	37.3207160036197	39.0	37.0	39.0	34.0	39.0
10	37.158626385414095	39.0	37.0	39.0	33.0	39.0
11	37.21476398560508	39.0	37.0	39.0	33.0	39.0
12	37.16095160668515	39.0	37.0	39.0	33.0	39.0
13	37.21605281792945	39.0	37.0	39.0	34.0	39.0
14	38.37986276113372	40.0	38.0	41.0	34.0	41.0
15	38.38869102059712	40.0	38.0	41.0	34.0	41.0
16	38.380933830800025	40.0	38.0	41.0	34.0	41.0
17	38.36915690364083	40.0	38.0	41.0	34.0	41.0
18	38.39463271504869	40.0	38.0	41.0	34.0	41.0
19	38.43509785608631	40.0	38.0	41.0	34.0	41.0
20	38.43492041984641	40.0	38.0	41.0	34.0	41.0
21	38.38959271930716	40.0	38.0	41.0	34.0	41.0
22	38.33019593800055	40.0	38.0	41.0	34.0	41.0
23	38.31328061863952	40.0	38.0	41.0	34.0	41.0
24	38.28167035250129	40.0	38.0	41.0	34.0	41.0
25	38.2561622799589	40.0	38.0	41.0	34.0	41.0
26	38.15489135256419	40.0	38.0	41.0	33.0	41.0
27	38.09530665014896	40.0	38.0	41.0	33.0	41.0
28	38.06824520397909	40.0	37.0	41.0	33.0	41.0
29	37.99935074466764	40.0	37.0	41.0	33.0	41.0
30	37.9205214689785	40.0	37.0	41.0	33.0	41.0
31	37.90365373479089	40.0	37.0	41.0	33.0	41.0
32	37.81170304916113	40.0	37.0	41.0	33.0	41.0
33	37.79313515318393	40.0	37.0	41.0	33.0	41.0
34	37.739876052721144	40.0	37.0	41.0	33.0	41.0
35	37.69224006800647	40.0	37.0	41.0	33.0	41.0
36	37.67281241279412	40.0	37.0	41.0	33.0	41.0
37	37.62467960660773	40.0	37.0	41.0	33.0	41.0
38	37.5710140158499	40.0	37.0	41.0	32.0	41.0
39	37.49983304862882	40.0	37.0	41.0	32.0	41.0
40	37.43650040890988	40.0	37.0	41.0	32.0	41.0
41	37.376405174040755	40.0	37.0	41.0	32.0	41.0
42	37.32506641761071	39.0	36.0	41.0	31.0	41.0
43	36.68979225442421	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	12.0
11	9.0
12	15.0
13	7.0
14	12.0
15	6.0
16	11.0
17	12.0
18	37.0
19	48.0
20	115.0
21	208.0
22	406.0
23	819.0
24	1396.0
25	2672.0
26	4151.0
27	6425.0
28	9600.0
29	13834.0
30	19185.0
31	25616.0
32	34046.0
33	44550.0
34	58787.0
35	80060.0
36	112186.0
37	168001.0
38	297232.0
39	360415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.50480771557293	21.525838749171292	15.000540374003332	23.96881316125244
2	14.069484031544938	22.421569149322274	40.91421603023513	22.594730788897653
3	15.117406333828542	28.282207500391166	33.81120138851923	22.789184777261063
4	12.099296545961632	18.94728691923909	38.6168199877085	30.33659654709077
5	12.54869414992717	38.24726869169808	35.85389577395268	13.350141384422065
6	27.830229005663444	40.037277740946315	18.015101437072236	14.117391816318003
7	24.888739412298914	32.92248778512794	23.169704859010775	19.01906794356237
8	24.383852656946388	35.464100616026364	22.150575619292802	18.001471107734446
9	24.689365600920087	15.510830869389183	19.91157223026062	39.8882312994301
10	14.459601800816529	29.145838071687468	33.740307545395446	22.654252582100554
11	31.51832190482643	23.653783182593184	25.304988700537635	19.52290621204276
12	21.100072426247014	26.212091150609496	30.136012943167174	22.55182347997632
13	28.430931330562103	21.663432487930304	27.071447121580928	22.834189059926672
14	21.797961418909217	22.089037505181945	27.46704928372216	28.645951792186676
15	23.639023713546933	28.799837403881984	23.787989502226825	23.773149380344258
16	23.70209423154784	28.206958404106196	24.142378065009414	23.94856929933655
17	21.416957420141593	29.557570801092364	25.7576124179559	23.267859360810142
18	23.17664100293415	26.676086917948645	26.734156960097817	23.41311511901939
19	23.95195671846192	26.809567362055418	26.840054134183738	22.398421785298925
20	23.349560684000576	25.91149802965121	27.124919952059955	23.614021334288264
21	23.408759865858205	26.48752058663647	26.853361852176256	23.250357695329072
22	21.33525609695116	30.058182956120017	26.398479855341073	22.208081091587747
23	22.574244968472808	27.05926854329686	27.121613185770904	23.24487330245943
24	23.563290700244053	27.74739854276455	26.429692502996254	22.25961825399514
25	22.04717868313275	28.406090256976068	26.549865229110516	22.99686583078067
26	23.21793525512912	26.72343013286748	26.763595245353994	23.295039366649405
27	21.911681918118013	28.941221825947956	26.369767445611757	22.777328810322274
28	21.31218938576413	27.009828354633747	28.335196413852287	23.342785845749837
29	22.42850529324565	27.57915672620459	26.324601857273517	23.66773612327625
30	21.30839870245717	28.194779825822135	27.449225006895816	23.047596464824878
31	23.586034800085816	26.12232454378723	27.68085995280196	22.61078070332499
32	21.767313341108267	29.01792267328665	27.220332257424495	21.994431728180587
33	21.550599169921007	27.45454809409282	28.268738476726014	22.726114259260154
34	22.94903869884392	27.43938536086499	26.948290240522887	22.663285699768203
35	21.771910552778408	27.893460829336984	28.86919884311572	21.46542977476889
36	22.812090182775457	26.440016066044997	26.40517404075549	24.342719710424056
37	22.83031772378339	27.08749703600827	27.400026776741658	22.682158463466685
38	22.656188250172193	25.89810965882237	28.102512981074007	23.34318910993143
39	23.430939395845733	26.248384926952728	27.112983332284845	23.207692344916694
40	21.655286551462154	26.140794043304123	29.786866814745274	22.417052590488453
41	22.436651229713796	26.205316312358757	27.10056279549183	24.257469662435618
42	23.122039032746663	25.686799227668438	28.363021642382098	22.828140097202798
43	22.77563510075959	25.318135112857515	28.856294389304786	23.04993539707811
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	14.5
2	24.0
3	110.5
4	197.0
5	197.0
6	258.0
7	319.0
8	330.5
9	342.0
10	587.0
11	832.0
12	832.0
13	1273.0
14	1714.0
15	4042.0
16	6370.0
17	7372.5
18	8375.0
19	8375.0
20	8484.5
21	8594.0
22	10650.5
23	12707.0
24	16351.0
25	19995.0
26	19995.0
27	25471.0
28	30947.0
29	38177.0
30	45407.0
31	50625.5
32	55844.0
33	55844.0
34	61026.5
35	66209.0
36	70388.5
37	74568.0
38	79230.0
39	83892.0
40	83892.0
41	86646.0
42	89400.0
43	87309.5
44	85219.0
45	86690.5
46	88162.0
47	88162.0
48	89601.5
49	91041.0
50	102886.5
51	114732.0
52	102173.0
53	89614.0
54	89614.0
55	88351.5
56	87089.0
57	74764.5
58	62440.0
59	50130.0
60	37820.0
61	37820.0
62	33178.0
63	28536.0
64	23778.0
65	19020.0
66	15678.5
67	12337.0
68	12337.0
69	9890.5
70	7444.0
71	6269.5
72	5095.0
73	3962.0
74	2829.0
75	2829.0
76	2132.5
77	1436.0
78	1113.5
79	791.0
80	535.5
81	280.0
82	280.0
83	204.0
84	128.0
85	105.0
86	82.0
87	54.5
88	27.0
89	27.0
90	22.5
91	18.0
92	9.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1239882.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.929482163424765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27885454497383	40.37631008484991
2	12.50354229410277	12.735978684942687
3	3.5436950420547912	5.414356603108388
4	1.420205497872123	2.893213222891043
5	0.7337480088927406	1.8684703065675636
6	0.4437072201745277	1.3558667373396838
7	0.3012082626474786	1.0738266587986647
8	0.21167660733261523	0.8624463998048556
9	0.18579145238066425	0.8516036214124039
>10	1.1264461173882805	11.277596488244882
>50	0.14441600546553007	5.062461172493395
>100	0.09892808903656013	9.120313029952113
>500	0.005081376442794095	1.7518592211341288
>1k	0.002064309179885101	2.0426765860680374
>5k	3.1758602767463094E-4	1.2034630721861772
>10k+	3.1758602767463094E-4	2.1095581102060637
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	14960	1.2065664313216902	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	11125	0.8972628040410296	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	8725	0.7036959968771221	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	6156	0.4964988603754228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4115	0.33188642144978314	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3621	0.29204392030854553	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	2949	0.23784521430265138	RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	2799	0.22574728885490716	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1825	0.14719142628088802	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	1656	0.1335610969430962	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1579	0.1273508285465875	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1362	0.10984916306551752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.065283631829481E-5	0.0
11	0.0	0.0	0.0	8.065283631829481E-5	0.0
12	0.0	0.0	0.0	1.6130567263658962E-4	0.0
13	0.0	0.0	0.0	1.6130567263658962E-4	0.0
14	0.0	0.0	0.0	2.419585089548844E-4	0.0
15	0.0	0.0	0.0	2.419585089548844E-4	0.0
16	0.0	0.0	0.0	4.0326418159147403E-4	0.0
17	0.0	0.0	0.0	5.645698542280636E-4	0.0
18	0.0	0.0	0.0	6.452226905463585E-4	0.0
19	0.0	0.0	0.0	6.452226905463585E-4	0.0
20	0.0	0.0	0.0	8.065283631829481E-4	0.0
21	0.0	0.0	0.0	0.0010484868721378324	0.0
22	0.0	0.0	0.0	0.0016130567263658961	0.0
23	0.0	0.0	0.0	0.0019356680716390753	0.0
24	0.0	0.0	0.0	0.0023389322532305495	0.0
25	0.0	0.0	0.0	0.0026615435985037285	0.0
26	0.0	0.0	0.0	0.002984154943776908	0.0
27	0.0	0.0	0.0	0.0036293776343232663	0.0
28	0.0	0.0	0.0	0.009275076176603902	0.0
29	0.0	0.0	0.0	0.02217952998753107	0.0
30	0.0	0.0	0.0	0.040568376668102285	0.0
31	0.0	0.0	0.0	0.08791159158694134	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1920	0.0	31.122395	1
TATACAC	2165	0.0	28.284065	3
TTATACA	2170	0.0	27.963135	2
GGTATCA	820	0.0	26.39634	1
CGTCTTC	470	0.0	26.372341	37
CCGTCTT	1390	0.0	25.154676	37
GCCGTCT	1415	0.0	24.710247	36
GTATCAA	2290	0.0	23.993448	1
TTACGGG	90	3.8289727E-9	22.611113	3
TGCCGTC	1730	0.0	20.104048	35
ATTACGG	95	1.6763806E-7	19.473684	2
GATTACG	95	1.6763806E-7	19.473684	1
ATGCCGT	1835	0.0	18.852861	34
ATACACA	3435	0.0	18.25764	4
TACACAT	3375	0.0	18.253334	5
TATGCCG	1950	0.0	17.646154	33
GTCTTAT	105	4.798021E-7	17.619047	1
ATTAGAC	100	5.8785427E-6	16.65	3
AACGGCC	90	4.446409E-5	16.444445	37
GCGACCC	80	3.382833E-4	16.1875	21
>>END_MODULE