FastQCFastQC Report
Fri 10 Feb 2017
ERR1631140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307866
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT168175.4624414518004585No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG123794.020905199015156No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT113403.6834207090097637RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC63432.0603119539020223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51711.679626850642812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49411.6049190232113972No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC47741.5506746441633699RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC36921.1992230385947134No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA32211.0462344006808157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20290.6590529646014824No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19180.6229983174497996No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16450.5343233744551201No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15450.5018417103545049No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14520.47163376274093277No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14090.4576666471776682No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG13860.4501958644345267No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA13540.43980173192232985No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13320.4326557658201945No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12560.40796970110372693No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11750.3816595531822286No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11430.37126542067003176No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT11420.3709406040290256No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10370.33683485672337965No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9150.2972072265206291No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8170.26537519570202617No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8170.26537519570202617No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8070.2621270292919647No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7820.2540066132668109No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7790.2530321633437924No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7610.24718546380568168No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7590.2465358305236694No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7390.24003949770354635No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7370.23938986442153407No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7260.23581688137046639No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6640.21567824962808496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.21470379970506648No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6570.21340453314104188No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6320.20528411711588807No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6050.196514067808722No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5810.18871846842457432No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5700.18514548537350667No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5460.177349885989359No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5370.17442653622030363No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5220.16955428660521138No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4880.1585105208110022No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4840.15721125424697757No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4840.15721125424697757No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4660.15136455470886684No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4660.15136455470886684No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4650.1510397380678607No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4550.1477915716577992No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4450.14454340524773765No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4420.1435689553247192No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4410.14324413868371305No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4350.14129523883767614No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4330.14064560555566383No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4230.1373974391456023No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4220.13707262250459615No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4170.13544853929956538No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.1347989060175531No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4110.1334996394535285No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4010.13025147304346696No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4000.12992665640246082No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3960.1286273898384362No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3940.1279777565564239No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3900.12667848999239928No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3890.12635367335139314No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3890.12635367335139314No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3890.12635367335139314No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3870.12570404006938082No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3700.12018215717227625No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3680.11953252389026395No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3680.11953252389026395No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3630.11790844068523319No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3620.11758362404422702No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3590.11660917412120858No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3560.11563472419819011No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3480.11303619107014092No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3310.10751430817303631No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3200.10394132512196864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3160.10264205855794403No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3150.10231724191693788No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3120.10134279199391943No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA3110.10101797535291329No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3100.10069315871190712No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3100.10069315871190712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAC200.001839843337.07
ATTACGG452.6066118E-937.02
TTACTTT551.2732926E-1137.03
TCAGAGT308.292216E-637.03
TTACGGG452.6066118E-937.03
GATTACG452.6066118E-937.01
CGGACTA200.001839843337.09
GTTTGTG251.2315273E-436.9999961
TTCTACA251.2315273E-436.9999962
CGTCTTC5950.036.06722637
CTTATAC16450.035.4255331
TTATACA17850.032.8543432
AGCAACG401.5950336E-632.3752
CCTAGTT401.5950336E-632.3752
TTATCAA401.5950336E-632.3752
AGTTCCT401.5950336E-632.3755
ACTCAGA352.3826733E-531.7142851
TCTACAC303.5934127E-430.8333323
TATACAC19350.030.2118873
CCGGACT250.005490172729.5999988
Produced by FastQC (version 0.11.5)