FastQCFastQC Report
Fri 10 Feb 2017
ERR1631139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340911
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG134533.946191234662419No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT99842.9286235997078416TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC79442.330226950729076No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT72672.131641396141515No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC37661.1046871470853097No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC35581.0436741554247297Illumina Paired End PCR Primer 2 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.9201815136501902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27530.8075421444306579No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG19250.5646635045510412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11410.3346914590611626No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA11380.333811463989135No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT10730.3147449040952037No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9980.2927450272945138No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9170.2689851603497687No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7530.22087876307892673No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7240.21237214404932667No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7130.20914549545189212No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5530.16221242494375365No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4840.14197253828711892No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4730.1387458896896844No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4370.12818594882535325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.11469269105426343No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3900.11439935936358755No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3780.11087937907547718No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3650.10706606709669092No Hit
ATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT3460.10149276497384949No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3450.10119943328317361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTCC200.001840120237.07
CCAACGT200.001840120237.017
TGATACA251.2318049E-436.9999962
AAGGGTT251.2318049E-436.9999965
CTTATAC9850.033.9949261
AGCAACG603.45608E-1133.9166682
TTATACA10250.032.1268272
GACAGGT352.3834178E-531.71428734
GCAACGC705.456968E-1231.7142873
GTATCGA352.3834178E-531.71428716
TCGAAGG352.3834178E-531.71428719
CCCTGTC352.3834178E-531.71428737
CGTCTTC4650.031.03225737
GATACAC303.5942154E-430.8333343
TCAAATT551.8477294E-830.27272829
GGTATCA3200.030.0625021
CCTGTCC250.005490989429.59999812
CGCTGAC250.005490989429.59999821
GGGCGCT250.005490989429.59999818
CCCCCTT250.005490989429.59999827
Produced by FastQC (version 0.11.5)