FastQCFastQC Report
Fri 10 Feb 2017
ERR1631138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17831
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT231212.96618249116707No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT217512.197857663619539TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG207211.620211990353878No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC12226.853233133307161No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC5453.0564746789299537RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC5312.977959733049184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2331.3067130278728059No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG1821.0206942964500028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1630.9141382984689586No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA1500.8412315630082441No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT1440.8075823004879144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1320.7402837754472548No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1210.6785934608266502No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC610.3421008356233526No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA550.30845157310302285No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA460.2579776793225282No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT390.21872020638214348No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.20750378554203353No Hit
CTGATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT370.20750378554203353No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT290.16263810218159386TruSeq Adapter, Index 10 (95% over 23bp)
TCGCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT280.1570298917615389TruSeq Adapter, Index 10 (95% over 21bp)
CGTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT200.11216420840109921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3050.037.01
TTATACA3300.034.1969682
TATACAC3550.032.8309863
CGTCTTC802.7284841E-1127.7537
GCCGTCT2600.026.32692336
CCGTCTT2650.025.83018937
TGCCGTC3150.022.31746135
GGTATCA753.3323704E-722.21
ATGCCGT3200.021.9687534
ATACACA5900.020.3813554
TATGCCG3450.020.37681233
TACACAT5950.019.899165
GTATCAA1452.3737812E-916.5862061
ACACATC7200.016.4444456
CACATCT7450.015.8926177
ACATCTC7500.015.7866668
CATCTCC8000.015.0312499
ATCTCCG8050.014.93788810
CGTATGC4600.014.88043531
AGTACGG1001.0006154E-414.79999913
Produced by FastQC (version 0.11.5)