FastQCFastQC Report
Fri 10 Feb 2017
ERR1631137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516281
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT223274.324582930613367No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG175633.401829623790145No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT102151.9785736837110022TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC95251.8449255347378655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84611.638836215161898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84401.6347686628018463No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA56571.0957211286101949No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC44530.8625147933005476No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA34780.6736641480124196No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC33490.6486777549435288RNA PCR Primer, Index 7 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29640.5741059616759091No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT29320.5679077866510679No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25280.48965582696244875No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT25080.4857819675719231No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC24140.46757482843645226No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT23880.46253881122876883No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG23170.4487866103924026No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG22960.44471905803235057No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC21440.41527772666435525No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG20270.3926156492297799No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG16930.3279221974080007No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG16790.3252104958346327No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA16690.32327356613936986No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT15650.3031294973086362No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14660.28395389332553395No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14100.273107087032062No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT13690.2651656752814843No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG13600.26342243855574776No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG13080.2533504041403809No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13060.2529630182013283No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13000.2518008603841706No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG12900.2498639306889078No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG12820.24831438693269753No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12520.24250359784690895No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11920.23088201967533187No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG11280.21848566962564958No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11160.21616135399133415No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11030.21364334538749247No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT10860.21035056490554563No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10480.2029902320635468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.1991163726730211No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10170.19698575000823196No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9550.18497678589760227No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9210.17839122493370857No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8800.1704498131831309No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8770.169868734274552No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8710.1687065764573943No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8410.16289578737160576No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8070.15631022640771208No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7810.15127420920002865No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC7680.14875620059618697No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7640.14798142871808181No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG7590.1470129638704504No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG7580.14681927090092411No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA7570.14662557793139783No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7560.14643188496187154No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.146044499022819No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7440.1441075693275561No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG7290.14120217478466185No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG7260.14062109587608299No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7230.14004001696750415No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7180.1390715521198727No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA7150.13849047321129385No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7050.136553543516031No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG6800.13171121927787388No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT6560.12706258800924303No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA6400.12396350049682246No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6350.12299503564919105No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA6280.12163918486250705No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT6170.1195085621977179No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6170.1195085621977179No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6140.11892748328913906No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG6120.11854009735008648No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6110.11834640438056018No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA6100.11815271141103392No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC6030.11679686062434991No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6010.11640947468529735No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5860.11350408014240308No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG5820.11272930826429794No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5770.1117608434166665No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC5740.11117976450808764No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC5720.11079237856903507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC5650.10943652778235109No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5590.10827436996519338No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5490.10633744026993053No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA5480.10614374730040424No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5450.10556266839182539No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG5430.10517528245277281No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5420.10498158948324653No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT5390.10440051057466768No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA5330.10323835275750998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG200.001840996837.016
TTACTTT308.303114E-637.03
CATTAGC200.001840996837.01
CATAGTA200.001840996837.01
TTACGGG1100.037.03
TCTCAAC308.303114E-637.03
ATAGTAC200.001840996837.02
ACTCAGA800.037.01
GATTACG1100.035.318181
CTTATAC25200.035.311511
TCAGAGT850.034.823533
CGTCTTC4800.033.91666837
ATTACGG1150.033.7826082
TTATACA26900.033.14872
GTTTGTG451.0780059E-732.888891
CTAAGTC352.3857785E-531.71428734
GTGTCAA352.3857785E-531.7142871
AAGTCTG352.3857785E-531.71428736
TATACAC28450.031.4077323
GGGTCCC303.5967564E-430.83333418
Produced by FastQC (version 0.11.5)