FastQCFastQC Report
Fri 10 Feb 2017
ERR1631136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141649
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT1959913.836313705003212No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG1575911.125387401252391No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1438710.156796023974755RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC82045.791781092700972No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC66574.699644896893025RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC44333.1295667459706737RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG17711.2502735635267457No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA17571.2403899780443208No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT13540.955883910228805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.7257375625666259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10000.7059703916017762No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9810.6925569541613424No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT7270.5132404746944913Illumina PCR Primer Index 11 (95% over 24bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT4660.32898220248642773Illumina PCR Primer Index 11 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4290.302861297997162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4010.2830941270323123No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA2840.20049559121490443No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2750.19414185769048847No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT2550.18002244985845295No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2520.17790453868364758No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2400.1694328939844263No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2210.15601945654399255No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2180.1539015453691872RNA PCR Primer, Index 39 (95% over 23bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2000.14119407832035524No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1840.1298985520547268No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1830.12919258166312506No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA1820.12848661127152328No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1710.12072093696390374No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1680.1186030257890984No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1660.11719108500589485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT308.260584E-637.037
CTTAATA200.001836491637.01
CTTATAC22050.036.66441
TTATACA24050.033.538462
CAGACAC401.5869864E-632.37518
TTTACAC401.5869864E-632.3752
TATACAC25900.031.1428593
CGGAGAT303.583701E-430.8333321
GATACAC303.583701E-430.8333323
TGATACA303.583701E-430.8333322
CGTCTTC8050.029.64596437
CCCGGGC250.00548028329.612
TAATACA250.00548028329.63
AATACAC250.00548028329.64
GACAGAC556.196806E-726.9090916
GGAGATG358.830888E-426.428572
CCCACGC358.830888E-426.4285719
GCCACCT358.830888E-426.4285726
ACAGACA509.0178055E-625.90000217
ACCGGGG659.2706614E-825.61538534
Produced by FastQC (version 0.11.5)