##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631136.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141649
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.060720513381668	31.0	31.0	34.0	28.0	34.0
2	31.249348742313746	31.0	31.0	34.0	28.0	34.0
3	30.75760506604353	31.0	31.0	34.0	27.0	34.0
4	34.80058454348425	37.0	35.0	37.0	32.0	37.0
5	34.94919131091642	35.0	35.0	37.0	32.0	37.0
6	35.10645327534963	36.0	35.0	37.0	32.0	37.0
7	35.04234410408827	36.0	35.0	37.0	32.0	37.0
8	35.096230824079235	37.0	35.0	37.0	32.0	37.0
9	36.97850320157573	39.0	37.0	39.0	33.0	39.0
10	36.772013921736125	39.0	37.0	39.0	32.0	39.0
11	36.7713856080876	39.0	37.0	39.0	32.0	39.0
12	36.63146227647213	39.0	35.0	39.0	32.0	39.0
13	36.75466116951055	39.0	37.0	39.0	32.0	39.0
14	37.43290810383412	39.0	36.0	40.0	32.0	41.0
15	37.42041948760669	39.0	36.0	40.0	32.0	41.0
16	37.35326052425361	39.0	36.0	40.0	31.0	41.0
17	37.17640788145345	39.0	36.0	40.0	31.0	41.0
18	37.465460398590885	39.0	36.0	40.0	32.0	41.0
19	37.39148176125493	39.0	36.0	40.0	31.0	41.0
20	37.512386250520656	39.0	37.0	40.0	32.0	41.0
21	37.540893334933536	39.0	37.0	41.0	32.0	41.0
22	37.16772444563675	39.0	36.0	40.0	31.0	41.0
23	37.43293634264979	39.0	36.0	40.0	32.0	41.0
24	37.06842971005796	39.0	36.0	40.0	31.0	41.0
25	36.92179260001836	39.0	35.0	40.0	30.0	41.0
26	36.93232567826105	39.0	35.0	40.0	30.0	41.0
27	36.52193802991903	38.0	35.0	40.0	30.0	41.0
28	36.914725836398425	39.0	35.0	40.0	30.0	41.0
29	36.83333450995065	39.0	35.0	40.0	30.0	41.0
30	36.88515979639814	39.0	35.0	40.0	30.0	41.0
31	36.91272088048627	39.0	35.0	40.0	31.0	41.0
32	36.446836899660426	38.0	35.0	40.0	30.0	41.0
33	36.39004864135998	38.0	35.0	40.0	30.0	41.0
34	36.24616481584762	38.0	35.0	40.0	30.0	41.0
35	36.251177205628	38.0	35.0	40.0	30.0	41.0
36	36.36745758882873	38.0	35.0	40.0	30.0	41.0
37	36.229157989113936	38.0	35.0	40.0	30.0	41.0
38	36.191713319543375	38.0	35.0	40.0	30.0	41.0
39	35.8665433571716	38.0	35.0	40.0	29.0	41.0
40	35.34008005704241	38.0	33.0	40.0	26.0	41.0
41	35.491771915085884	38.0	34.0	40.0	27.0	41.0
42	35.36832593241039	38.0	34.0	40.0	26.0	41.0
43	34.14170237700231	37.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	21.0
11	23.0
12	12.0
13	16.0
14	19.0
15	11.0
16	12.0
17	18.0
18	15.0
19	40.0
20	37.0
21	81.0
22	104.0
23	200.0
24	397.0
25	648.0
26	961.0
27	1474.0
28	2170.0
29	2889.0
30	3823.0
31	4878.0
32	6097.0
33	7413.0
34	9466.0
35	11710.0
36	15226.0
37	20819.0
38	30139.0
39	22914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.425121250414758	26.990660011719108	20.424429399430988	34.15978933843515
2	7.01099195899724	15.959166672549754	49.95164102817528	27.078200340277732
3	8.651667149079767	31.81667360870885	43.420708935467246	16.110950306744133
4	5.721890023932396	25.61613565927045	18.094021136753526	50.56795318004363
5	11.389420327711456	35.85835410062902	32.413218589612356	20.339006982047174
6	22.848025753799885	37.467260623089466	13.817252504429964	25.867461118680684
7	15.990229369780232	37.78282938813546	10.181504987680817	36.04543625440349
8	26.00583131543463	28.917253210400357	26.594610622030512	18.4823048521345
9	12.427196803366067	10.431418506307844	9.297630057395393	67.8437546329307
10	12.069975785215568	27.86888717887172	25.3577504959442	34.70338653996851
11	21.466441697435208	16.041765208367163	25.447408735677623	37.044384358520006
12	11.941489173944046	28.031966339331728	13.559573311495315	46.46697117522891
13	36.88059922766839	10.057960169150505	25.08242204321951	27.979018559961595
14	22.75483766210845	29.169990610593793	12.362247527338702	35.712924199959055
15	30.144229751004243	24.396924792974183	10.236570678225755	35.22227477779582
16	39.464450860930896	18.694801940006638	10.265515464281428	31.575231734781045
17	12.69828943374115	45.81041871103926	10.599439459509068	30.891852395710522
18	29.823719193217034	10.721572337256175	12.25776390938164	47.19694456014515
19	18.586788470091562	25.968414884679735	17.132489463391906	38.31230718183679
20	12.561331177770404	16.46675938411143	23.50599015877274	47.46591927934543
21	28.571327718515487	12.557801325812395	17.609725448114705	41.26114550755741
22	10.64391559417998	43.35505368904828	27.00760330111755	18.99342741565419
23	23.488340898982695	11.544027843472245	18.51266157897338	46.45496967857168
24	25.34998482163658	28.217636552322993	28.170336536085678	18.262042089954747
25	11.304703880719243	38.28053851421471	19.65421570219345	30.760541902872596
26	24.785208508355158	17.148726782398747	17.67467472414207	40.39138998510403
27	9.865936222634824	38.95615217897762	36.7231678303412	14.454743768046368
28	8.643195504380547	11.806648829148106	26.489421033681847	53.0607346327895
29	9.697209299041997	14.536636333472174	37.09097840436572	38.675175963120104
30	9.845463081278371	16.94046551687622	42.983007292674145	30.231064109171264
31	16.014938333486295	10.72369024843098	27.22927800408051	46.03209341400222
32	19.557497758544006	28.725935234276275	38.32925047123524	13.387316535944482
33	9.754392900761742	16.35239218067194	32.726669443483544	41.16654547508278
34	12.802773051698214	34.38499389335611	34.21838488093809	18.593848174007583
35	14.739955806253485	27.054197346963267	44.821354192405174	13.384492654378075
36	21.787658225614017	15.338618698331791	14.600879639107935	48.27284343694625
37	17.030829727001247	26.230329899963994	29.982562531327435	26.75627784170732
38	18.233097303899072	11.381654653403835	34.99283440052524	35.392413642171846
39	33.27591440814972	15.649951641028176	21.013914676418473	30.060219274403632
40	12.175165373564232	10.88182761614978	56.22559989834026	20.717407111945725
41	21.523625299154954	20.85718924948288	19.64927390945224	37.96991154190994
42	25.10783697731717	11.234106841559065	44.87006614942569	18.787990031698072
43	23.229249765264846	11.672514454743768	40.03416896695352	25.06406681303786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	20.0
4	37.0
5	37.0
6	59.5
7	82.0
8	92.0
9	102.0
10	149.0
11	196.0
12	196.0
13	293.0
14	390.0
15	972.5
16	1555.0
17	1585.5
18	1616.0
19	1616.0
20	1320.0
21	1024.0
22	804.0
23	584.0
24	646.0
25	708.0
26	708.0
27	825.0
28	942.0
29	897.0
30	852.0
31	852.5
32	853.0
33	853.0
34	938.0
35	1023.0
36	1228.5
37	1434.0
38	1421.0
39	1408.0
40	1408.0
41	1534.0
42	1660.0
43	2031.0
44	2402.0
45	3232.5
46	4063.0
47	4063.0
48	7031.5
49	10000.0
50	26739.5
51	43479.0
52	26284.5
53	9090.0
54	9090.0
55	20270.5
56	31451.0
57	25200.0
58	18949.0
59	11201.5
60	3454.0
61	3454.0
62	2747.0
63	2040.0
64	1543.0
65	1046.0
66	844.5
67	643.0
68	643.0
69	465.5
70	288.0
71	223.5
72	159.0
73	108.5
74	58.0
75	58.0
76	38.5
77	19.0
78	20.0
79	21.0
80	14.5
81	8.0
82	8.0
83	6.5
84	5.0
85	4.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	141649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.806331142471883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5148281945728	12.523208776623909
2	9.85465848945644	3.312413077395534
3	4.221624800470469	2.1285007306793555
4	2.51617239351424	1.691505058277856
5	1.419810131899521	1.1930899618070019
6	1.096362261614719	1.1055496332483816
7	0.8695286902461564	1.0229510974309737
8	0.6594976056456356	0.8866988118518309
9	0.5670839284214063	0.8577540257961581
>10	3.7805595228093756	12.458965470988147
>50	0.3066453835167605	3.636453487140749
>100	0.13862051583634377	4.359367168140968
>500	0.012601865076031254	1.91176782045761
>1k	0.021003108460052086	7.301851760337172
>5k	0.008401243384020835	10.491425989593997
>10k+	0.012601865076031254	35.11849713023036
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	19599	13.836313705003212	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	15759	11.125387401252391	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	14387	10.156796023974755	RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	8204	5.791781092700972	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	6657	4.699644896893025	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	4433	3.1295667459706737	RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1771	1.2502735635267457	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1757	1.2403899780443208	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1354	0.955883910228805	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1028	0.7257375625666259	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1000	0.7059703916017762	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	981	0.6925569541613424	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	727	0.5132404746944913	Illumina PCR Primer Index 11 (95% over 24bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	466	0.32898220248642773	Illumina PCR Primer Index 11 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	429	0.302861297997162	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	401	0.2830941270323123	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	284	0.20049559121490443	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	275	0.19414185769048847	No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	255	0.18002244985845295	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	252	0.17790453868364758	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	240	0.1694328939844263	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	221	0.15601945654399255	No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	218	0.1539015453691872	RNA PCR Primer, Index 39 (95% over 23bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	200	0.14119407832035524	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	184	0.1298985520547268	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	183	0.12919258166312506	No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	182	0.12848661127152328	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	171	0.12072093696390374	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	168	0.1186030257890984	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	166	0.11719108500589485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.059703916017762E-4	0.0
8	0.0	0.0	0.0	7.059703916017762E-4	0.0
9	0.0	0.0	0.0	7.059703916017762E-4	0.0
10	0.0	0.0	0.0	7.059703916017762E-4	0.0
11	0.0	0.0	0.0	7.059703916017762E-4	0.0
12	0.0	0.0	0.0	7.059703916017762E-4	0.0
13	0.0	0.0	0.0	0.0014119407832035523	0.0
14	0.0	0.0	0.0	0.0014119407832035523	0.0
15	0.0	0.0	0.0	0.0014119407832035523	0.0
16	0.0	0.0	0.0	0.0014119407832035523	0.0
17	0.0	0.0	0.0	0.0014119407832035523	0.0
18	0.0	0.0	0.0	0.0014119407832035523	0.0
19	0.0	0.0	0.0	0.0014119407832035523	0.0
20	0.0	0.0	0.0	0.0014119407832035523	0.0
21	0.0	0.0	0.0	0.0014119407832035523	0.0
22	0.0	0.0	0.0	0.0021179111748053287	0.0
23	0.0	0.0	0.0	0.003529851958008881	0.0
24	0.0	0.0	0.0	0.0042358223496106575	0.0
25	0.0	0.0	0.0	0.0042358223496106575	0.0
26	0.0	0.0	0.0	0.0042358223496106575	0.0
27	0.0	0.0	0.0	0.005647763132814209	0.0
28	0.0	0.0	0.0	0.01835523018164618	0.0
29	0.0	0.0	0.0	0.045182105062513675	0.0
30	0.0	0.0	0.0	0.06283136485255808	0.0
31	0.0	0.0	0.0	0.0889522693418238	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTGCT	30	8.260584E-6	37.0	37
CTTAATA	20	0.0018364916	37.0	1
CTTATAC	2205	0.0	36.6644	1
TTATACA	2405	0.0	33.53846	2
CAGACAC	40	1.5869864E-6	32.375	18
TTTACAC	40	1.5869864E-6	32.375	2
TATACAC	2590	0.0	31.142859	3
CGGAGAT	30	3.583701E-4	30.833332	1
GATACAC	30	3.583701E-4	30.833332	3
TGATACA	30	3.583701E-4	30.833332	2
CGTCTTC	805	0.0	29.645964	37
CCCGGGC	25	0.005480283	29.6	12
TAATACA	25	0.005480283	29.6	3
AATACAC	25	0.005480283	29.6	4
GACAGAC	55	6.196806E-7	26.90909	16
GGAGATG	35	8.830888E-4	26.42857	2
CCCACGC	35	8.830888E-4	26.42857	19
GCCACCT	35	8.830888E-4	26.42857	26
ACAGACA	50	9.0178055E-6	25.900002	17
ACCGGGG	65	9.2706614E-8	25.615385	34
>>END_MODULE