FastQCFastQC Report
Fri 10 Feb 2017
ERR1631135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429229
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG145133.3811788113105123No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT112022.6097957034589925RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC86492.01500830558979No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT70411.6403831055217613No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC42630.993176136747517No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC39730.925613134247686RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG20400.47527077620570835No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA11080.2581372647234926No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10910.25417667492177837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10830.2523128679562658No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT10690.24905120576661877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.24019812268043397No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG5290.12324423559451948No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5110.11905066992211617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4500.10483914181008272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9800.032.469391
CGTCTTC4800.031.98958237
CGGTGTA303.5957553E-430.83333234
TTATACA11350.028.3612332
TATACAC12200.026.8401623
CCGTCTT17050.026.47507337
TAGTCGG358.860305E-426.42857230
GCCGTCT17150.026.21282836
GGTATCA2650.025.8301891
GCCGCTA400.00192922723.12533
CGCAGCT400.00192922723.12529
TCGGTGT400.00192922723.12533
AGTAACG400.00192922723.12524
GTATCAA6300.021.4365061
TGCCGTC21750.020.58390835
AGTCCGA450.00382196120.55555718
CGAGTAA450.00382196120.55555722
TCCGAGT450.00382196120.55555720
CGGGGTA450.00382196120.55555712
ACGTCGG450.00382196120.55555728
Produced by FastQC (version 0.11.5)