##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631135.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429229
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.25398796446652	31.0	30.0	31.0	26.0	34.0
2	30.259227591798318	31.0	30.0	33.0	26.0	34.0
3	29.690055890911378	31.0	30.0	33.0	25.0	34.0
4	33.93975243983981	35.0	33.0	37.0	28.0	37.0
5	33.56828639257832	35.0	33.0	37.0	28.0	37.0
6	33.873934426611434	35.0	33.0	37.0	29.0	37.0
7	33.96413802422483	35.0	33.0	37.0	30.0	37.0
8	34.02145940744917	35.0	33.0	37.0	30.0	37.0
9	35.64173436557176	37.0	35.0	39.0	30.0	39.0
10	35.26841150062088	37.0	34.0	39.0	30.0	39.0
11	35.44334143312777	37.0	34.0	39.0	30.0	39.0
12	35.299078114479684	37.0	34.0	39.0	30.0	39.0
13	35.49224772790282	37.0	35.0	39.0	30.0	39.0
14	36.271920117233456	38.0	35.0	40.0	30.0	41.0
15	36.2812647794068	38.0	35.0	40.0	30.0	41.0
16	36.28269292149412	38.0	35.0	40.0	30.0	41.0
17	36.15304417921436	38.0	34.0	40.0	30.0	41.0
18	36.28733846035566	38.0	35.0	40.0	30.0	41.0
19	36.27086473653924	38.0	34.0	40.0	30.0	41.0
20	36.31181257557155	38.0	35.0	40.0	30.0	41.0
21	36.24810998324904	38.0	34.0	40.0	30.0	41.0
22	36.00885075332748	38.0	34.0	40.0	29.0	41.0
23	36.10977590050999	38.0	34.0	40.0	30.0	41.0
24	35.9492182494659	38.0	34.0	40.0	29.0	41.0
25	35.92918465434535	38.0	34.0	40.0	29.0	41.0
26	35.742226177634784	38.0	34.0	40.0	29.0	41.0
27	35.554599060175335	38.0	34.0	40.0	27.0	41.0
28	35.628326604213605	38.0	34.0	40.0	28.0	41.0
29	35.48285414079664	38.0	34.0	40.0	27.0	41.0
30	35.42813043853048	38.0	34.0	40.0	27.0	41.0
31	35.43275500956366	38.0	34.0	40.0	27.0	41.0
32	35.21091538549352	38.0	34.0	40.0	27.0	40.0
33	35.150602592089534	38.0	33.0	40.0	27.0	40.0
34	34.99013580163503	37.0	33.0	40.0	27.0	40.0
35	35.021163528093396	37.0	33.0	40.0	27.0	40.0
36	35.086226699500735	38.0	33.0	40.0	27.0	40.0
37	34.96492548266776	37.0	33.0	40.0	26.0	40.0
38	34.92180630852063	37.0	33.0	40.0	26.0	40.0
39	34.85668489314562	37.0	33.0	40.0	26.0	40.0
40	34.63837019399901	37.0	33.0	39.0	26.0	40.0
41	34.67770351024745	37.0	33.0	39.0	26.0	40.0
42	34.621686791898966	37.0	33.0	39.0	26.0	40.0
43	33.778169694964696	36.0	31.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	11.0
10	12.0
11	19.0
12	7.0
13	12.0
14	14.0
15	15.0
16	17.0
17	33.0
18	58.0
19	92.0
20	175.0
21	353.0
22	653.0
23	1221.0
24	1875.0
25	2934.0
26	4437.0
27	6577.0
28	8974.0
29	12262.0
30	16117.0
31	20888.0
32	26429.0
33	33461.0
34	42378.0
35	52218.0
36	61312.0
37	62899.0
38	52526.0
39	21250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.32014146294868	26.11869188708125	16.65311523685492	22.908051413115142
2	14.298195135929772	23.274522457709054	39.452366918358265	22.97491548800291
3	15.743810413555467	30.44901439557905	34.18454950620764	19.62262568465784
4	12.05999594621985	19.679005845364593	34.49067980029309	33.77031840812247
5	11.630854392410578	40.30575753269234	34.25910178482815	13.804286290068937
6	26.611901805330024	38.87342187969592	17.774894054222802	16.739782260751255
7	22.24010959184957	35.82236987715182	21.669085732790656	20.26843479820795
8	24.76020958509327	34.116986503707814	23.98696267027624	17.135841240922677
9	22.046273667436264	15.43651524011658	18.43701147872115	44.08019961372601
10	14.101563501068195	28.06916587649017	33.598149239683245	24.23112138275839
11	29.879388391744264	22.90339189570136	23.98370100808659	23.23351870446778
12	18.69072220190155	28.171209307851985	27.61160126645683	25.526467223789634
13	30.27358356495018	19.963935335217332	25.576790011858474	24.185691087974018
14	21.407220854136135	24.82823853933448	24.79632084505008	28.968219761479304
15	24.460602615387124	29.70488946459815	21.66535811885963	24.169149801155093
16	25.289763739169537	27.60717472491374	21.66582407060101	25.437237465315715
17	19.747267775476494	32.882913316667796	23.31902084901067	24.050798058845047
18	22.35333586500446	25.81535730344408	24.309867227051292	27.521439604500163
19	22.909449268339277	31.824969887868715	25.472183845919076	19.793396997872932
20	21.94190047736756	26.50473290481305	27.05618679073408	24.497179827085308
21	27.704092687120397	26.301810921442865	23.447390553760346	22.54670583767639
22	20.45970798804368	32.12923637498864	24.98689510727374	22.42416052969394
23	26.428549795097723	25.247362130704122	24.395835323335564	23.928252750862594
24	21.651146590747597	31.735973105265487	24.249526476542822	22.36335382744409
25	22.45677715159041	29.1068404045393	26.33139885702038	22.10498358684991
26	26.49751065282169	28.975209037599974	23.74070717495789	20.786573134620447
27	19.747500751347182	30.123547104226418	28.211281157610507	21.917670986815896
28	25.651575266349663	24.94589135403246	27.41962914900904	21.982904230608835
29	19.90382756057955	27.92402190905088	25.345444972264225	26.826705558105346
30	23.486530500036114	27.051993225061683	29.344242816771466	20.11723345813074
31	25.005999128670243	24.33619350043916	24.479939612654317	26.177867758236278
32	22.08494766197065	29.99680823057156	26.458370706545924	21.459873400911867
33	24.94193076423075	25.180498055816358	29.34331091328871	20.53426026666418
34	20.667755440569017	30.384946031139553	25.537184113841327	23.410114414450096
35	20.864154099559908	26.566471510545654	33.31718033963222	19.252194050262215
36	24.78583693086907	25.59705891260842	23.978109587190055	25.638994569332453
37	21.99618385523811	25.90365515843524	29.254547106556174	22.84561387977047
38	21.689121657669915	24.420297789757914	28.16491895934338	25.72566159322879
39	25.86847580196119	25.88897767858183	26.203961055753457	22.038585463703527
40	20.186194315854706	23.88655007000925	34.287058889310835	21.64019672482521
41	22.69604337078809	27.50093772787952	25.448187331238103	24.354831570094284
42	21.28374364267093	24.38162379522353	32.504793478539426	21.829839083566117
43	23.46230100948445	23.76563559312162	29.339816275228376	23.432247122165556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	11.5
2	14.0
3	36.5
4	59.0
5	59.0
6	87.5
7	116.0
8	123.5
9	131.0
10	204.0
11	277.0
12	277.0
13	409.0
14	541.0
15	1287.5
16	2034.0
17	2371.5
18	2709.0
19	2709.0
20	2954.0
21	3199.0
22	4096.5
23	4994.0
24	6388.5
25	7783.0
26	7783.0
27	9477.5
28	11172.0
29	13750.0
30	16328.0
31	17743.5
32	19159.0
33	19159.0
34	21281.5
35	23404.0
36	24342.5
37	25281.0
38	26590.0
39	27899.0
40	27899.0
41	28625.0
42	29351.0
43	28054.5
44	26758.0
45	26446.0
46	26134.0
47	26134.0
48	25672.5
49	25211.0
50	26482.0
51	27753.0
52	37718.0
53	47683.0
54	47683.0
55	34236.5
56	20790.0
57	28494.5
58	36199.0
59	29047.5
60	21896.0
61	21896.0
62	15128.0
63	8360.0
64	6741.0
65	5122.0
66	4235.5
67	3349.0
68	3349.0
69	2669.0
70	1989.0
71	1810.5
72	1632.0
73	1352.0
74	1072.0
75	1072.0
76	765.0
77	458.0
78	337.5
79	217.0
80	151.5
81	86.0
82	86.0
83	60.5
84	35.0
85	24.0
86	13.0
87	10.5
88	8.0
89	8.0
90	6.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	429229.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.26156194921888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7686709490463	44.083886952071424
2	10.322411206454579	10.995754878757834
3	2.8462927114023366	4.547939867218972
4	1.1754349894287202	2.5042201402694886
5	0.6428490106710131	1.7119571202924115
6	0.411186617371096	1.3140264896280236
7	0.27981184749624954	1.0432251234682817
8	0.20370915487724597	0.8679894217693968
9	0.15335042780958927	0.735091497964769
>10	1.0437565769729007	10.936852134117677
>50	0.1032822357354915	3.8077411516774045
>100	0.04314321239572526	3.9651107646434607
>500	8.715800483984901E-4	0.2413929166556927
>1k	0.0034863201935939605	3.6343560471104195
>5k	8.715800483984901E-4	3.6417835214690566
>10k+	8.715800483984901E-4	5.96867197288571
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	14513	3.3811788113105123	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	11202	2.6097957034589925	RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	8649	2.01500830558979	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	7041	1.6403831055217613	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	4263	0.993176136747517	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	3973	0.925613134247686	RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	2040	0.47527077620570835	No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	1108	0.2581372647234926	No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	1091	0.25417667492177837	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1083	0.2523128679562658	No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT	1069	0.24905120576661877	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1031	0.24019812268043397	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	529	0.12324423559451948	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	511	0.11905066992211617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	450	0.10483914181008272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.3297587068907275E-4	0.0
6	0.0	0.0	0.0	2.3297587068907275E-4	0.0
7	0.0	0.0	0.0	2.3297587068907275E-4	0.0
8	0.0	0.0	0.0	2.3297587068907275E-4	0.0
9	0.0	0.0	0.0	2.3297587068907275E-4	0.0
10	0.0	0.0	0.0	2.3297587068907275E-4	0.0
11	0.0	0.0	0.0	2.3297587068907275E-4	0.0
12	0.0	0.0	0.0	2.3297587068907275E-4	0.0
13	0.0	0.0	0.0	2.3297587068907275E-4	0.0
14	0.0	0.0	0.0	2.3297587068907275E-4	0.0
15	0.0	0.0	0.0	2.3297587068907275E-4	0.0
16	0.0	0.0	0.0	2.3297587068907275E-4	0.0
17	0.0	0.0	0.0	2.3297587068907275E-4	0.0
18	0.0	0.0	0.0	2.3297587068907275E-4	0.0
19	0.0	0.0	0.0	2.3297587068907275E-4	0.0
20	0.0	0.0	0.0	2.3297587068907275E-4	0.0
21	0.0	0.0	0.0	4.659517413781455E-4	0.0
22	0.0	0.0	0.0	4.659517413781455E-4	0.0
23	0.0	0.0	0.0	6.989276120672182E-4	0.0
24	0.0	0.0	0.0	6.989276120672182E-4	0.0
25	0.0	0.0	0.0	6.989276120672182E-4	0.0
26	0.0	0.0	0.0	9.31903482756291E-4	0.0
27	0.0	0.0	0.0	0.0011648793534453636	0.0
28	0.0	0.0	0.0	0.003960589801714236	0.0
29	0.0	0.0	0.0	0.010017962439630127	0.0
30	0.0	0.0	0.0	0.019104021396503964	0.0
31	0.0	0.0	0.0	0.04589624652574733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	980	0.0	32.46939	1
CGTCTTC	480	0.0	31.989582	37
CGGTGTA	30	3.5957553E-4	30.833332	34
TTATACA	1135	0.0	28.361233	2
TATACAC	1220	0.0	26.840162	3
CCGTCTT	1705	0.0	26.475073	37
TAGTCGG	35	8.860305E-4	26.428572	30
GCCGTCT	1715	0.0	26.212828	36
GGTATCA	265	0.0	25.830189	1
GCCGCTA	40	0.001929227	23.125	33
CGCAGCT	40	0.001929227	23.125	29
TCGGTGT	40	0.001929227	23.125	33
AGTAACG	40	0.001929227	23.125	24
GTATCAA	630	0.0	21.436506	1
TGCCGTC	2175	0.0	20.583908	35
AGTCCGA	45	0.003821961	20.555557	18
CGAGTAA	45	0.003821961	20.555557	22
TCCGAGT	45	0.003821961	20.555557	20
CGGGGTA	45	0.003821961	20.555557	12
ACGTCGG	45	0.003821961	20.555557	28
>>END_MODULE