FastQCFastQC Report
Fri 10 Feb 2017
ERR1631134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553587
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG137572.4850655813810656No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT115742.0907282866107764TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT93631.6913330695988165No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC75471.363290684210431No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC44460.8031257959453527RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC39110.7064833531134221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18460.3334615877901757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17770.32099742226605754No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG17610.3181071809851026No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA12290.22200665839335101No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT11230.20285880990702454No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT10320.18642056262159334No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7800.14089926244655313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6950.12554485564148002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12350.032.056681
CGTCTTC5950.031.71428537
ATGACGT250.005493912429.625
TTATACA13700.028.8978122
TATACAC14500.027.8137933
GGTATCA3550.026.056341
CCGTCTT18500.025.837
GCCGTCT18900.025.35185236
AACGGCC601.3353147E-624.66666637
TTAACGG551.9003495E-523.54545435
GTATCAA9650.023.3886011
TAACGTA400.001929928923.12500218
ACCGACC502.6996506E-422.28
TGCCGTC22300.021.48654735
ATGCCGT23100.020.74242234
CGAACTA450.003823339720.55555724
GAGTCGA450.003823339720.55555725
ACGTAAT450.003823339720.55555720
TTTCGCA555.138466E-420.18181826
TGAGCGT555.138466E-420.18181835
Produced by FastQC (version 0.11.5)