FastQCFastQC Report
Fri 10 Feb 2017
ERR1631133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330281
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG145164.395045431011775No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT144164.364768182244816No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT93842.8412170242914367Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC80782.4457961553949517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58361.76698023803973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55391.6770568092018614No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC37871.146599410804739No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA34531.0454733999230958No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC28380.8592683200062977Illumina PCR Primer Index 11 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA21310.6452081712238972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20190.6112976526049031No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG19770.5985812081227804No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19400.5873786260790054No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17360.525613038594409No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16020.48504152524668387No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14840.4493143717016722No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14710.4453783293619676No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14190.42963416000314886No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13330.40359572606356403No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA12930.3914848265567804No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG12430.3763462021733009No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11490.34788558833235944No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT11160.3378940962392629No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9880.29913921781755537No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT9610.29096436065047643No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9550.2891477257244589No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9220.2791562336313624No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9120.2761285087546665No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9030.2734035563656402No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9020.27310078387797054No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8880.2688619690505963No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8220.24887898486440335No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8150.2467595774507162No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8010.24252076262334193No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7560.22889600067821037No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7540.22829045570287118No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7150.21648232868375716No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7070.21406014878240046No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6960.21072965141803496No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6760.20467420166464315No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6670.20194924927561683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.18771894235514605No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6110.18499398996611977No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5940.17984685767573672No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5750.17409418041001448No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5520.16713041319361394No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5340.1616805084155613No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5190.15713892110051744No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5130.1553222861744999No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4990.15108347134712563No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4970.15047792637178645No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4970.15047792637178645No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4950.14987238139644726No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4900.14835851895809932No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4840.14654188403208177No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4740.14351415915538587No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4740.14351415915538587No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4570.13836702686500282No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4560.13806425437733325No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.13715593691432446No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4510.13655039193898527No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4490.13594484696364612No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4450.13473375701296775No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4400.13321989457461977No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4280.1295866247225847No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4280.1295866247225847No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4170.12625612735821923No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4160.12595335487054962No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4110.12443949243220168No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4050.12262285750618412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3920.11868681516647944No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3850.11656740775279231No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3840.11626463526512272No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3830.11596186277745314No Hit
ATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCT3750.1135396828760964Illumina PCR Primer Index 11 (95% over 21bp)
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3660.1108147304870701No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3630.10990641302406133No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3610.10930086804872213No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3590.10869532307338298No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3560.1077870056103742No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3560.1077870056103742No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3550.10748423312270461No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3550.10748423312270461No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3520.10657591565969583No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA3470.10506205322134787No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA3400.10294264580766074No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG3340.10112601088164322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG200.00184003737.01
TTTACAC403.81915E-837.02
ATTACTT308.294048E-637.02
GGGTAAT200.00184003737.01
TTACGGG551.2732926E-1137.03
CGGTCTG200.00184003737.019
CCTATTT200.00184003737.017
CGCCGAG200.00184003737.02
CCAGGCA251.2317215E-436.99999624
CTGGTTT251.2317215E-436.99999616
TCTACAC251.2317215E-436.9999963
TCAGAGT251.2317215E-436.9999963
ACTCAGA251.2317215E-436.9999961
TATAACA251.2317215E-436.9999963
CTTATAC16700.035.559881
GATTACG603.45608E-1133.9166641
CGTCTTC3100.032.22580337
TTATACA18750.031.7706642
ATTACGG658.731149E-1131.3076922
TCCAGGC303.593975E-430.83333223
Produced by FastQC (version 0.11.5)