FastQCFastQC Report
Fri 10 Feb 2017
ERR1631132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120058
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT66965.577304302920255RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG59694.971763647570341No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT50954.243782171950224No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC32202.682037015442536No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC23841.9857069083276415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22801.8990821103133486No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC21921.8257842043012544RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19531.6267137550184076No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10630.8854053873960919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8820.7346449216212164No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG7370.6138699628512885No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6340.5280780955871329No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA5970.49725965783204784No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5800.4830998350797115No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5470.45561312032517615No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5150.42895933632077826No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5010.41729830581885424No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4810.40063969081610556No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4370.3639907378100585No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4330.36065901480950874No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4090.3406686768062103No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3910.3256759233037365No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3650.30401972380016323No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3020.2515450865415049No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2840.23655233303903114No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2780.23155474853820657No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2710.2257242332872445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.22405837178696963No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2690.22405837178696963No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2620.21822785653600757No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT2530.2107314797847707No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2410.20073631078312149No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2360.19657165703243432No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2350.19573872628229688No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2320.19323993403188458No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2160.17991304202968564No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2110.17574838827899847No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2090.17408252677872363No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2070.17241666527844876No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2040.16991787302803646No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1990.1657532192773493No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1960.16325442702693696No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG1950.16242149627679955No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.15825684252611238No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1870.15575805027570008No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1800.14992753502473805No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1690.14076529677322627No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1640.1366006430225391No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1630.13576771227240167No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1620.13493478152226424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1610.1341018507721268No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG1610.1341018507721268No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1590.13243598927185193No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1570.13077012777157707No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1570.13077012777157707No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA1500.12493961252061504No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1490.1241066817704776No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG1470.12244082027020274No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1450.12077495876992787No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1440.11994202801979043No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1420.11827616651951557No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1420.11827616651951557No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1420.11827616651951557No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1410.11744323576937812No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1400.1166103050192407No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG1350.11244565126855353No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA1330.11077978976827867No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1310.10911392826800378No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1310.10911392826800378No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG1280.1066151360175915No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1270.10578220526745406No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC1270.10578220526745406No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1240.10328341301704178No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA1220.1016175515167669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACA308.25009E-637.017
GACAGAC308.25009E-637.016
ACCGTTT200.001835378737.010
CAGACAC308.25009E-637.018
AGACAGA308.25009E-637.015
CGTCTTC2300.034.58695637
CTTATAC6000.034.5333331
GACACAT352.3706647E-531.71428720
TTATACA6800.030.4705892
CAGCAGC250.00547699929.615
GTTCATA250.00547699929.61
GCAGCCG250.00547699929.617
TAACTCC250.00547699929.64
CTAACTC250.00547699929.63
GCTAACT250.00547699929.62
CAGCCGC250.00547699929.618
TATACAC7300.028.6369883
GGTATCA2200.028.5909081
CCGTCTT9500.027.84736837
ATAAGAG405.896602E-527.7510
Produced by FastQC (version 0.11.5)