##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631132.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	120058
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.120708324309916	31.0	31.0	34.0	28.0	34.0
2	31.112945409718638	31.0	31.0	34.0	28.0	34.0
3	30.522205933798663	31.0	30.0	34.0	26.0	34.0
4	34.70825767545686	35.0	35.0	37.0	32.0	37.0
5	34.94492661880091	35.0	35.0	37.0	32.0	37.0
6	35.16920155258292	36.0	35.0	37.0	32.0	37.0
7	35.13949924203302	37.0	35.0	37.0	32.0	37.0
8	35.175823352046514	37.0	35.0	37.0	32.0	37.0
9	36.94157823718536	39.0	37.0	39.0	33.0	39.0
10	36.808251012010864	39.0	37.0	39.0	32.0	39.0
11	36.82401839111096	39.0	37.0	39.0	32.0	39.0
12	36.68958336803878	39.0	35.0	39.0	32.0	39.0
13	36.820436788885374	39.0	37.0	39.0	32.0	39.0
14	37.60436622299222	39.0	37.0	40.0	32.0	41.0
15	37.624015059387965	39.0	37.0	40.0	32.0	41.0
16	37.65250129104266	39.0	37.0	40.0	32.0	41.0
17	37.710498259174734	39.0	37.0	40.0	32.0	41.0
18	37.846807376434725	39.0	37.0	41.0	33.0	41.0
19	37.808442586083395	39.0	37.0	41.0	33.0	41.0
20	37.77838211531093	39.0	37.0	41.0	33.0	41.0
21	37.69660497426244	39.0	37.0	41.0	32.0	41.0
22	37.47799396958137	39.0	36.0	40.0	32.0	41.0
23	37.509661996701595	39.0	36.0	40.0	32.0	41.0
24	37.3654566959303	39.0	36.0	40.0	32.0	41.0
25	37.30576887837545	39.0	36.0	40.0	32.0	41.0
26	37.034008562528115	39.0	35.0	40.0	31.0	41.0
27	36.87141215079378	39.0	35.0	40.0	31.0	41.0
28	36.83824484832331	39.0	35.0	40.0	31.0	41.0
29	36.755551483449665	39.0	35.0	40.0	31.0	41.0
30	36.49865065218478	38.0	35.0	40.0	30.0	41.0
31	36.26852021522931	38.0	35.0	40.0	30.0	41.0
32	35.9654666910993	38.0	35.0	40.0	30.0	41.0
33	35.65921471288877	38.0	35.0	40.0	28.0	41.0
34	35.4594029552383	38.0	34.0	40.0	27.0	41.0
35	35.40622865614953	38.0	34.0	40.0	27.0	41.0
36	35.23427843209116	38.0	34.0	40.0	26.0	41.0
37	35.130195405553984	38.0	34.0	40.0	26.0	41.0
38	34.99239534225124	38.0	34.0	40.0	26.0	41.0
39	34.6436305785537	38.0	33.0	40.0	24.0	41.0
40	34.15767379100102	37.0	33.0	40.0	22.0	41.0
41	34.07560512418998	37.0	33.0	40.0	22.0	41.0
42	33.78394609272185	37.0	32.0	40.0	21.0	41.0
43	32.746647453730695	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	10.0
11	17.0
12	10.0
13	4.0
14	8.0
15	9.0
16	7.0
17	13.0
18	14.0
19	22.0
20	40.0
21	77.0
22	127.0
23	263.0
24	427.0
25	726.0
26	1059.0
27	1539.0
28	2123.0
29	2692.0
30	3406.0
31	4200.0
32	5132.0
33	6516.0
34	8089.0
35	10359.0
36	13878.0
37	19797.0
38	25193.0
39	14294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.3733861966716	22.86977960652351	18.892535274617266	23.86429892218761
2	12.304885971780307	22.83229772276733	40.728647820220225	24.134168485232138
3	14.67957154042213	30.9042296223492	36.272468306984955	18.143730530243715
4	9.307168202035683	19.12658881540589	28.983491312532276	42.582751670026155
5	13.411018007962818	37.40775291942228	29.111762648053443	20.069466424561462
6	26.671275550150757	35.454530310349995	15.724899631844607	22.149294507654634
7	21.89691649036299	34.80734311749321	16.36375751720002	26.931982874943778
8	29.758949840910226	27.18519382298556	24.108347631977878	18.94750870412634
9	20.887404421196422	11.64104016392077	15.42920921554582	52.04234619933699
10	16.402072331706343	25.68758433423845	27.225174498992153	30.685168835063052
11	30.356994119508908	19.896216828532875	21.28138066601143	28.465408385946795
12	19.124922953905614	28.546202668710123	22.056839194389376	30.272035182994884
13	35.39705808859052	16.355428209698648	23.280414466341266	24.967099235369574
14	23.830148761431975	25.386063402688702	24.14333072348365	26.640457112395676
15	28.16138866214663	25.985773542787655	19.931199920038647	25.92163787502707
16	28.519548884705724	21.87775908310983	19.147412084159324	30.455279948025122
17	18.681803794832497	33.5187992470306	20.226890336337437	27.572506621799462
18	25.28861050492262	16.2854620266871	25.670092788485565	32.75583467990471
19	23.770177747422082	26.121541255060055	29.646504189641675	20.46177680787619
20	20.35016408735778	26.661280381149112	24.19497242999217	28.793583101500943
21	29.48324976261474	23.8218194539306	26.07406420230222	20.62086658115244
22	25.86999616851855	33.018207866198004	24.24161655199987	16.87017941328358
23	29.701477619150747	19.942860950540574	25.276949474420697	25.07871195588799
24	26.144863316063905	23.83181462293225	26.94114511319529	23.08217694780856
25	26.39307667960486	29.01430975028736	23.752686201669192	20.839927368438588
26	23.736860517416584	28.994319412284064	28.77442569424778	18.494394376051577
27	23.8151560079295	33.562944576787885	26.659614519648837	15.962284895633777
28	18.62349864232288	27.09023971746989	30.772626563827483	23.51363507637975
29	23.613586766396242	21.617051758316812	29.00514751203585	25.7642139632511
30	24.88047443735528	24.981259058121907	32.54843492312049	17.589831581402322
31	22.98888870379317	24.585616951806628	22.57991970547569	29.84557463892452
32	32.16861850105782	20.460943877126056	28.602009028969334	18.768428592846792
33	22.012693864632094	24.93461493611421	33.23310399973346	19.81958719952023
34	17.655633110663178	29.653167635642774	26.34643255759716	26.34476669609689
35	22.85145513002049	20.656682603408353	39.433440503756515	17.05842176281464
36	23.608589181895418	25.611787635975947	24.605607289809925	26.174015892318714
37	20.986523180462775	23.780172916423727	30.473604424528144	24.75969947858535
38	21.03400023322061	18.381115794032883	29.768945009911874	30.815938962834633
39	29.022639057788734	19.494744206966633	26.700428126405573	24.78218860883906
40	17.939662496460045	19.613020373486147	40.31551416815206	22.131802961901748
41	23.9709140582052	21.567075913308567	24.89963184460844	29.56237818387779
42	22.12430658515051	18.581852104816004	37.65430042146296	21.639540888570526
43	23.86679771443802	18.747605324093357	32.7266821036499	24.65891485781872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	34.5
4	65.0
5	65.0
6	109.5
7	154.0
8	178.5
9	203.0
10	299.0
11	395.0
12	395.0
13	625.5
14	856.0
15	2119.5
16	3383.0
17	3359.0
18	3335.0
19	3335.0
20	2789.0
21	2243.0
22	1733.0
23	1223.0
24	1187.5
25	1152.0
26	1152.0
27	1126.0
28	1100.0
29	1040.5
30	981.0
31	1007.0
32	1033.0
33	1033.0
34	1165.5
35	1298.0
36	1304.5
37	1311.0
38	1415.5
39	1520.0
40	1520.0
41	1844.5
42	2169.0
43	2975.0
44	3781.0
45	5306.5
46	6832.0
47	6832.0
48	9280.0
49	11728.0
50	16381.0
51	21034.0
52	16078.5
53	11123.0
54	11123.0
55	15526.0
56	19929.0
57	16445.5
58	12962.0
59	8609.0
60	4256.0
61	4256.0
62	3485.5
63	2715.0
64	2086.5
65	1458.0
66	1216.5
67	975.0
68	975.0
69	765.0
70	555.0
71	389.5
72	224.0
73	131.0
74	38.0
75	38.0
76	24.5
77	11.0
78	9.5
79	8.0
80	4.5
81	1.0
82	1.0
83	2.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	120058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.015059387962484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35141509433963	17.855536490696164
2	10.269839067702554	4.932615902313882
3	4.217536071032186	3.038531376501358
4	2.3238068812430632	2.232254410368322
5	1.5607658157602664	1.8740941878092257
6	0.991953385127636	1.429309167235836
7	0.7665094339622641	1.2885438704626098
8	0.6867369589345172	1.3193623082176948
9	0.495976692563818	1.071981875426877
>10	3.610571587125416	17.179196721584567
>50	0.4370144284128746	7.204850988688801
>100	0.22891231964483907	10.717319962018358
>500	0.02774694783573807	4.158823235436206
>1k	0.02081021087680355	10.90472938079928
>5k	0.010405105438401775	14.79285012244082
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	6696	5.577304302920255	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	5969	4.971763647570341	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	5095	4.243782171950224	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	3220	2.682037015442536	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2384	1.9857069083276415	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2280	1.8990821103133486	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2192	1.8257842043012544	RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1953	1.6267137550184076	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1063	0.8854053873960919	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	882	0.7346449216212164	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	737	0.6138699628512885	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	634	0.5280780955871329	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	597	0.49725965783204784	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	580	0.4830998350797115	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	547	0.45561312032517615	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	515	0.42895933632077826	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	501	0.41729830581885424	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	481	0.40063969081610556	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	437	0.3639907378100585	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	433	0.36065901480950874	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	409	0.3406686768062103	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	391	0.3256759233037365	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	365	0.30401972380016323	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	302	0.2515450865415049	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	284	0.23655233303903114	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	278	0.23155474853820657	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	271	0.2257242332872445	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	269	0.22405837178696963	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	269	0.22405837178696963	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	262	0.21822785653600757	No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT	253	0.2107314797847707	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	241	0.20073631078312149	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	236	0.19657165703243432	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	235	0.19573872628229688	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	232	0.19323993403188458	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	216	0.17991304202968564	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	211	0.17574838827899847	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	209	0.17408252677872363	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	207	0.17241666527844876	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	204	0.16991787302803646	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	199	0.1657532192773493	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	196	0.16325442702693696	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	195	0.16242149627679955	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	190	0.15825684252611238	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	187	0.15575805027570008	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	180	0.14992753502473805	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	169	0.14076529677322627	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	164	0.1366006430225391	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	163	0.13576771227240167	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	162	0.13493478152226424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	161	0.1341018507721268	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	161	0.1341018507721268	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	159	0.13243598927185193	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	157	0.13077012777157707	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	157	0.13077012777157707	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	150	0.12493961252061504	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	149	0.1241066817704776	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	147	0.12244082027020274	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	145	0.12077495876992787	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	144	0.11994202801979043	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	142	0.11827616651951557	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	142	0.11827616651951557	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	142	0.11827616651951557	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	141	0.11744323576937812	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	140	0.1166103050192407	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	135	0.11244565126855353	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	133	0.11077978976827867	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	131	0.10911392826800378	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	131	0.10911392826800378	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	128	0.1066151360175915	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	127	0.10578220526745406	No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC	127	0.10578220526745406	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	124	0.10328341301704178	No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA	122	0.1016175515167669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.329307501374336E-4	0.0
11	0.0	0.0	0.0	8.329307501374336E-4	0.0
12	0.0	0.0	0.0	0.0016658615002748671	0.0
13	0.0	0.0	0.0	0.0016658615002748671	0.0
14	0.0	0.0	0.0	0.0016658615002748671	0.0
15	0.0	0.0	0.0	0.0016658615002748671	0.0
16	0.0	0.0	0.0	0.002498792250412301	0.0
17	0.0	0.0	0.0	0.004164653750687168	0.0
18	0.0	0.0	0.0	0.004997584500824602	0.0
19	0.0	0.0	0.0	0.004997584500824602	0.0
20	0.0	0.0	0.0	0.0066634460010994686	0.0
21	0.0	0.0	0.0	0.008329307501374335	0.0
22	0.0	0.0	0.0	0.010828099751786637	0.0
23	0.0	0.0	0.0	0.019990338003298407	0.0
24	0.0	0.0	0.0	0.022489130253710705	0.0
25	0.0	0.0	0.0	0.02332206100384814	0.0
26	0.0	0.0	0.0	0.024154991753985573	0.0
27	0.0	0.0	0.0	0.024154991753985573	0.0
28	0.0	0.0	0.0	0.03914774525645938	0.0
29	0.0	0.0	0.0	0.06746739076113212	0.0
30	0.0	0.0	0.0	0.11494444351896584	0.0
31	0.0	0.0	0.0	0.17991304202968564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACA	30	8.25009E-6	37.0	17
GACAGAC	30	8.25009E-6	37.0	16
ACCGTTT	20	0.0018353787	37.0	10
CAGACAC	30	8.25009E-6	37.0	18
AGACAGA	30	8.25009E-6	37.0	15
CGTCTTC	230	0.0	34.586956	37
CTTATAC	600	0.0	34.533333	1
GACACAT	35	2.3706647E-5	31.714287	20
TTATACA	680	0.0	30.470589	2
CAGCAGC	25	0.005476999	29.6	15
GTTCATA	25	0.005476999	29.6	1
GCAGCCG	25	0.005476999	29.6	17
TAACTCC	25	0.005476999	29.6	4
CTAACTC	25	0.005476999	29.6	3
GCTAACT	25	0.005476999	29.6	2
CAGCCGC	25	0.005476999	29.6	18
TATACAC	730	0.0	28.636988	3
GGTATCA	220	0.0	28.590908	1
CCGTCTT	950	0.0	27.847368	37
ATAAGAG	40	5.896602E-5	27.75	10
>>END_MODULE