FastQCFastQC Report
Fri 10 Feb 2017
ERR1631131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632411
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT151982.4031840053383005No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG121941.9281764548687483No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT109981.7390589347750118RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC64151.0143719827770232No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC44930.7104557004859182RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC33060.5227613055433887RNA PCR Primer, Index 27 (95% over 21bp)
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA26130.413180668900446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.34629378679371486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21440.3390200360208788No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG14550.23007189944513928No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA11760.18595501975772086No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT9640.15243251619595485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8860.14009876488549378No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT8090.12792313859183346No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6980.11037126172694656No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC6760.10689251135732933No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA6700.10594376125652462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18450.034.2926831
GATTACG507.3214323E-933.31
CGTCTTC6050.032.1074437
TTATACA20700.030.3864752
GACGAAC250.005494498629.59999822
TATACAC22300.029.2847543
GGTATCA4200.028.630951
TCGCCGA451.3222925E-424.6666681
CCGTCTT18400.024.63315237
GCTTTAT4450.024.528091
GCCGTCT18650.024.30294836
GTATCAA11350.022.6563871
TTATTGA5400.020.898154
ATTGAGC5200.020.6346156
TTAACGG450.00382393320.55555735
TGCCGTC22100.020.5090535
TATTGAG5500.020.1818185
TTTATTG6150.020.154473
ATGCCGT23350.019.49036434
CCGCCTT951.6731428E-719.47368436
Produced by FastQC (version 0.11.5)