FastQCFastQC Report
Fri 10 Feb 2017
ERR1631130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959682
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT135351.4103630160824105No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG111771.164656625840643No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT93660.9759482828687003Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC64420.6712640228742438No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC35150.36626715932986137Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC31240.325524496656184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29670.3091649108767279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29410.30645568011070334No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC24180.25195846124028587No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG15460.1610950293951538No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11860.12358260340404426No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA10520.10961964484068681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10270.10701461525797087No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT10050.10472218922518084No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG9850.1026381655590081No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA9720.10128355017599579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17500.030.7628571
TATACAC18950.028.7994733
TTATACA19150.027.9190622
GATTACG754.620233E-1027.1333331
TTACGGG809.786163E-1025.43753
CCGTCTT13250.025.13207437
CGTCTTC4500.025.07777837
GTATCAA14000.024.3142851
GGTATCA5000.024.051
GCCGTCT13850.023.90974836
TGGACCG753.739442E-722.25
TTTGTCG705.1004736E-621.14285935
TAACGCG450.003825354620.5555554
GCGGGTA656.9006135E-519.92307722
TGCCGTC16850.019.7626135
TTGTCGT759.263156E-619.73333436
TGTCGTA759.263156E-619.73333437
ATGCCGT17300.018.82080834
CGGTGTA801.6162177E-518.534
GGACCGT801.6162177E-518.56
Produced by FastQC (version 0.11.5)