##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	959682
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47470203671633	31.0	31.0	34.0	28.0	34.0
2	31.47484375032563	31.0	31.0	34.0	28.0	34.0
3	30.943705310717508	31.0	31.0	34.0	27.0	34.0
4	35.09857640343364	37.0	35.0	37.0	32.0	37.0
5	35.29672224757785	37.0	35.0	37.0	33.0	37.0
6	35.484190596468416	37.0	35.0	37.0	33.0	37.0
7	35.49099389172663	37.0	35.0	37.0	33.0	37.0
8	35.51124851773817	37.0	35.0	37.0	33.0	37.0
9	37.289306249361765	39.0	37.0	39.0	34.0	39.0
10	37.1217799229328	39.0	37.0	39.0	33.0	39.0
11	37.1786227104395	39.0	37.0	39.0	33.0	39.0
12	37.11608949631232	39.0	37.0	39.0	33.0	39.0
13	37.18998584947931	39.0	37.0	39.0	33.0	39.0
14	38.327717931564834	40.0	38.0	41.0	34.0	41.0
15	38.329808207302	40.0	38.0	41.0	34.0	41.0
16	38.33355944990112	40.0	38.0	41.0	34.0	41.0
17	38.302993074789356	40.0	38.0	41.0	33.0	41.0
18	38.326632155234755	40.0	38.0	41.0	33.0	41.0
19	38.35172171615181	40.0	38.0	41.0	34.0	41.0
20	38.34743383745866	40.0	38.0	41.0	34.0	41.0
21	38.290631688413455	40.0	38.0	41.0	34.0	41.0
22	38.208434668984104	40.0	38.0	41.0	33.0	41.0
23	38.218400470155736	40.0	38.0	41.0	34.0	41.0
24	38.17834032523273	40.0	38.0	41.0	33.0	41.0
25	38.17894573410776	40.0	38.0	41.0	33.0	41.0
26	38.05178694609256	40.0	37.0	41.0	33.0	41.0
27	37.9766537248797	40.0	37.0	41.0	33.0	41.0
28	37.96514783021876	40.0	37.0	41.0	33.0	41.0
29	37.86507197175731	40.0	37.0	41.0	33.0	41.0
30	37.80683601442978	40.0	37.0	41.0	33.0	41.0
31	37.76355605294254	40.0	37.0	41.0	33.0	41.0
32	37.673208416954786	40.0	37.0	41.0	33.0	41.0
33	37.650113266686255	40.0	37.0	41.0	32.0	41.0
34	37.583338022386584	40.0	37.0	41.0	32.0	41.0
35	37.56019181353823	40.0	37.0	41.0	32.0	41.0
36	37.54096982125329	40.0	37.0	41.0	32.0	41.0
37	37.46986189175164	40.0	37.0	41.0	32.0	41.0
38	37.427564547423	39.0	37.0	41.0	32.0	41.0
39	37.33603631202836	39.0	36.0	41.0	31.0	41.0
40	37.25170629437668	39.0	36.0	41.0	31.0	41.0
41	37.18446214475212	39.0	36.0	41.0	31.0	41.0
42	37.12885414126763	39.0	36.0	41.0	31.0	41.0
43	36.477278931979555	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	8.0
11	14.0
12	9.0
13	3.0
14	2.0
15	7.0
16	5.0
17	16.0
18	22.0
19	31.0
20	96.0
21	160.0
22	342.0
23	689.0
24	1252.0
25	2112.0
26	3464.0
27	5282.0
28	7858.0
29	11609.0
30	15709.0
31	21118.0
32	27748.0
33	35976.0
34	48045.0
35	64259.0
36	88945.0
37	131619.0
38	228726.0
39	264548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.47962137458033	21.658424353066955	14.967145366902788	24.89480890544993
2	14.212833000931557	21.405944885910124	40.86155622383248	23.51966588932584
3	14.760097615668524	28.013654523060765	33.595711912904484	23.63053594836623
4	12.065976021223698	18.529992226591723	37.98966741066312	31.414364341521463
5	12.854570576503466	38.2561098363833	35.124655875592126	13.764663711521107
6	28.536640262086816	39.4094085332433	17.5995798608289	14.45437134384098
7	25.27951967422542	32.617679606369606	22.869137901930014	19.233662817474954
8	25.222000621039054	34.85050256230709	21.81555973749638	18.11193707915747
9	24.531563580436018	14.843250159948818	19.56606459222951	41.059121667385654
10	15.25213560325191	28.275720499081984	33.057304398748755	23.41483949891735
11	31.810016234544356	23.090044410544326	24.97150097636509	20.128438378546225
12	21.43230778528721	25.914000679391712	29.03044966978645	23.623241865534624
13	28.549665410000397	20.461256958034017	26.83128369605765	24.157793935907936
14	21.902880329109017	21.6372715128553	26.652057660766797	29.807790497268886
15	24.029418078071696	28.363041090694622	23.00126500236537	24.606275828868313
16	24.379742456355334	26.900160678224662	23.659920682059266	25.06017618336074
17	21.413447371108347	29.627209846595022	25.120508668496438	23.838834113800196
18	23.8279971907361	25.470624644413466	25.924629200089193	24.776748964761243
19	24.34243843273084	26.411561329690457	25.756656892595668	23.489343344983027
20	24.22156506009282	25.84918754337374	25.644849022905504	24.28439837362793
21	25.725500738786387	25.774996300857993	25.33974795817781	23.159755002177803
22	22.772960209736144	29.56406392950998	25.71737304648832	21.94560281426556
23	24.701932515145643	25.094145769119354	25.833765768244067	24.37015594749094
24	23.424842812514978	26.894950619059234	25.568573756723584	24.11163281170221
25	23.461104824306382	26.723331270149902	26.318509672995848	23.497054232547864
26	24.413399438564024	25.89493186284623	26.831700500790888	22.859968197798857
27	23.679823108071215	28.23268541037552	25.512826123653458	22.574665357899804
28	23.694202871367807	25.04100316563195	27.508382985197176	23.756410977803064
29	22.570809914117383	26.33059701025965	26.287457720369872	24.811135355253093
30	24.86511156820697	25.36767387530453	27.179107245941886	22.58810731054662
31	24.35244174632847	25.05798795851126	26.485752572206213	24.103817722954062
32	22.45796003259413	27.220683518082033	26.782309139902594	23.539047309421246
33	23.41327648116772	25.789480265337893	28.491625350897486	22.305617902596904
34	22.378975535646184	27.169416535894182	26.282039258837823	24.169568669621814
35	22.65927671874642	25.283791922741074	29.286471977175772	22.770459381336735
36	23.86363399542765	25.708515945907084	26.451574584080976	23.976275474584288
37	22.802553345795793	26.350603637454906	27.523804760326858	23.32303825642244
38	23.327727309671328	25.142390916991253	27.535371091674115	23.9945106816633
39	23.84925423213106	25.840017839242584	26.626319968489565	23.684407960136795
40	22.234240092030486	24.7014115092291	30.422577478789847	22.641770919950567
41	22.81516168897614	25.55711162655963	26.640803932969465	24.986922751494767
42	22.88185044629367	25.33537150847885	28.52653274730588	23.256245297921605
43	23.448809084675965	24.105901746620233	29.01554890057331	23.429740268130487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	19.5
2	29.0
3	93.5
4	158.0
5	158.0
6	220.5
7	283.0
8	280.5
9	278.0
10	421.0
11	564.0
12	564.0
13	875.0
14	1186.0
15	2936.0
16	4686.0
17	5254.0
18	5822.0
19	5822.0
20	5862.0
21	5902.0
22	7281.0
23	8660.0
24	11124.0
25	13588.0
26	13588.0
27	17095.5
28	20603.0
29	24552.5
30	28502.0
31	33227.0
32	37952.0
33	37952.0
34	42845.5
35	47739.0
36	52137.0
37	56535.0
38	59856.5
39	63178.0
40	63178.0
41	65002.0
42	66826.0
43	67930.5
44	69035.0
45	69253.0
46	69471.0
47	69471.0
48	68020.5
49	66570.0
50	64754.0
51	62938.0
52	62023.5
53	61109.0
54	61109.0
55	72066.5
56	83024.0
57	64782.0
58	46540.0
59	47758.5
60	48977.0
61	48977.0
62	42528.5
63	36080.0
64	26111.0
65	16142.0
66	13081.5
67	10021.0
68	10021.0
69	8424.0
70	6827.0
71	6019.0
72	5211.0
73	6261.5
74	7312.0
75	7312.0
76	6324.0
77	5336.0
78	3661.5
79	1987.0
80	1180.5
81	374.0
82	374.0
83	250.0
84	126.0
85	94.0
86	62.0
87	47.5
88	33.0
89	33.0
90	18.5
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	959682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.43517951098241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3747651554242	37.17721054278636
2	12.36763741231438	11.733222015597493
3	3.732617695208155	5.311721712542047
4	1.5982091134208187	3.0324534476481833
5	0.862651837641349	2.0460022386998125
6	0.5277706467687203	1.5020937222060922
7	0.3800666828186075	1.2619971927950984
8	0.2526379211894697	0.9587140114323138
9	0.21056426843555578	0.898933848465535
>10	1.4175655977346968	13.419764675564489
>50	0.1637316120856585	5.398152424135045
>100	0.10323401731930941	9.022504752112749
>500	0.005479512589198525	1.8638462632011057
>1k	0.0021918050356794097	2.1605728340258925
>5k	4.3836100713588196E-4	1.643536661386907
>10k+	4.3836100713588196E-4	2.5692736574008883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	13535	1.4103630160824105	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	11177	1.164656625840643	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	9366	0.9759482828687003	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	6442	0.6712640228742438	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	3515	0.36626715932986137	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	3124	0.325524496656184	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2967	0.3091649108767279	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2941	0.30645568011070334	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2418	0.25195846124028587	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	1546	0.1610950293951538	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1186	0.12358260340404426	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1052	0.10961964484068681	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.10701461525797087	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1005	0.10472218922518084	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	985	0.1026381655590081	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	972	0.10128355017599579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0420118330863765E-4	0.0	1.0420118330863765E-4	0.0
6	0.0	1.0420118330863765E-4	0.0	1.0420118330863765E-4	0.0
7	0.0	1.0420118330863765E-4	0.0	1.0420118330863765E-4	0.0
8	0.0	1.0420118330863765E-4	0.0	1.0420118330863765E-4	0.0
9	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
10	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
11	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
12	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
13	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
14	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
15	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
16	0.0	1.0420118330863765E-4	0.0	2.084023666172753E-4	0.0
17	0.0	1.0420118330863765E-4	0.0	3.1260354992591297E-4	0.0
18	0.0	1.0420118330863765E-4	0.0	3.1260354992591297E-4	0.0
19	0.0	1.0420118330863765E-4	0.0	3.1260354992591297E-4	0.0
20	0.0	1.0420118330863765E-4	0.0	4.168047332345506E-4	0.0
21	0.0	1.0420118330863765E-4	0.0	6.252070998518259E-4	1.0420118330863765E-4
22	0.0	1.0420118330863765E-4	0.0	0.0010420118330863766	1.0420118330863765E-4
23	0.0	1.0420118330863765E-4	0.0	0.0013546153830122894	1.0420118330863765E-4
24	0.0	1.0420118330863765E-4	0.0	0.0018756212995554777	1.0420118330863765E-4
25	0.0	1.0420118330863765E-4	0.0	0.0018756212995554777	1.0420118330863765E-4
26	0.0	1.0420118330863765E-4	0.0	0.002084023666172753	1.0420118330863765E-4
27	0.0	1.0420118330863765E-4	0.0	0.0029176331326418543	1.0420118330863765E-4
28	0.0	1.0420118330863765E-4	0.0	0.0073982840149132735	1.0420118330863765E-4
29	0.0	1.0420118330863765E-4	0.0	0.019902426011949793	1.0420118330863765E-4
30	0.0	1.0420118330863765E-4	0.0	0.03719982244118364	1.0420118330863765E-4
31	0.0	1.0420118330863765E-4	0.0	0.07877609458133007	1.0420118330863765E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1750	0.0	30.762857	1
TATACAC	1895	0.0	28.799473	3
TTATACA	1915	0.0	27.919062	2
GATTACG	75	4.620233E-10	27.133333	1
TTACGGG	80	9.786163E-10	25.4375	3
CCGTCTT	1325	0.0	25.132074	37
CGTCTTC	450	0.0	25.077778	37
GTATCAA	1400	0.0	24.314285	1
GGTATCA	500	0.0	24.05	1
GCCGTCT	1385	0.0	23.909748	36
TGGACCG	75	3.739442E-7	22.2	5
TTTGTCG	70	5.1004736E-6	21.142859	35
TAACGCG	45	0.0038253546	20.555555	4
GCGGGTA	65	6.9006135E-5	19.923077	22
TGCCGTC	1685	0.0	19.76261	35
TTGTCGT	75	9.263156E-6	19.733334	36
TGTCGTA	75	9.263156E-6	19.733334	37
ATGCCGT	1730	0.0	18.820808	34
CGGTGTA	80	1.6162177E-5	18.5	34
GGACCGT	80	1.6162177E-5	18.5	6
>>END_MODULE