FastQCFastQC Report
Fri 10 Feb 2017
ERR1631129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218186
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT145396.663580614704885No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG118445.428395955744182No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT98944.534663085624192RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC62192.850320368859597No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC36921.6921342340938463RNA PCR Primer, Index 26 (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32641.4959713272162283No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC32471.4881798098869772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31151.4276809694480856No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21770.9977725426929316No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG13930.6384460964498181No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12920.5921553170230903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12370.5669474668402189No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA11710.536698046620773No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11070.5073652754988863No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10590.4853656971574712No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT10320.4729909343404251No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9600.4399915668283024No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9140.4189086375844463No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8760.401492304730826No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8240.37765942819429293No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7840.35932644624311366RNA PCR Primer, Index 26 (95% over 21bp)
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7620.3492433061699651No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7510.3442017361333908No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5920.2713281328774532No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5780.26491158919454044No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5530.25345347547505337No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5520.2529951509262739No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5500.25207850182871494No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5440.24932855453603806No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5380.24657860724336117No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5230.23970373901166891No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5200.2383287653653305No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5090.2332871953287562No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4770.21862080976781278No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4440.20349609965808987No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4360.199829503267854No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4200.1924963104873823No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3990.18287149496301322No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3920.17966322312155683No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3890.17828824947521837No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.17691327582887995No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3760.17233003034108513No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3410.15628867113380326No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3370.15445537293868533No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3310.15170542564600847No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3300.15124710109722897No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3300.15124710109722897No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3240.14849715380455208No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3170.14528888196309572No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3140.14391390831675727No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3070.14070563647530088No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT2960.1356640664387266RNA PCR Primer, Index 26 (95% over 23bp)
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2790.1278725491094754No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2770.12695590001191645No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2750.12603925091435747No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2740.125580926365578No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2670.12237265452412162No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2650.12145600542656267No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2650.12145600542656267No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2640.12099768087778318No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2610.11962270723144475No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2600.11916438268266526No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.11824773358510628No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2570.1177894090363268No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2500.11458113719487042No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2480.11366448809731146No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2450.11228951445097302No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA2380.10908124260951665No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2330.10678961986561925No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA2320.10633129531683976No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG2300.1054146462192808No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2290.10495632167050131No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG2290.10495632167050131No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2270.10403967257294235No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2250.1031230234753834No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2230.10220637437782443No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2230.10220637437782443No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2210.10128972528026547No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2210.10128972528026547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG251.2303492E-437.01
ACAGACA452.5993359E-937.017
GACAGAC452.5993359E-937.016
ATAACAC200.001838669337.04
ACTCAGA251.2303492E-437.01
GTCTTCT308.281126E-637.037
CTTATAA200.001838669337.01
CAGACAC452.5993359E-937.018
GATTACG355.6034696E-737.01
AGACAGA501.7826096E-1037.015
CTTATAC15850.035.9495241
CGTCTTC3750.034.03999737
TTATACA16900.033.7159772
ATTACGG401.5922124E-632.3752
TTACGGG401.5922124E-632.3753
GAGATGT401.5922124E-632.3751
TATACAC18350.031.4550423
TCAGAGT303.5900087E-430.8333343
TATAACA250.005486706329.63
ATTGATA250.005486706329.611
Produced by FastQC (version 0.11.5)