FastQCFastQC Report
Fri 10 Feb 2017
ERR1631128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256649
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG116624.54394912896602No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT111094.328479752502445No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT86223.359452014229551TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC63932.4909506758257387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44471.7327166675108807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41761.627124983927465No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC32431.2635934681218317No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC31451.2254090216599325RNA PCR Primer, Index 12 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22410.8731769849093509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15720.6125096922255687No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG15050.586403999236311No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14370.5599086690382585No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12450.4850983249496394No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA11230.43756258547666266No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11210.4367833110590729No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10450.40717088319066114No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9910.38613047391573707No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9680.3771688181134546No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9380.3654797018496078No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT9300.3623626041792487No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9110.35495949721214576No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8260.32184033446458005No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7330.28560407404665517No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6850.2669014880245004No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6660.2594983810573975No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6150.23962688340885804No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6090.23728906015608864No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5830.2271584927274215No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5680.22131393459549814No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5620.21897611134272876No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5600.21819683692513903No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5420.21118336716683095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.20339062299093313No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5040.1963771532326251No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5030.19598751602383022No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4960.19326005556226597No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4830.1881947718479324No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4810.1874154974303426No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4710.1835191253423937No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4520.17611601837529078No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4450.17338855791372654No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4440.17299892070493164No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4320.16832327419939294No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4290.16715436257300828No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4220.16442690211144403No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3970.15468597189157177No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3930.15312742305639218No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3590.1398797579573659No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3520.13715229749580166No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3430.13364556261664765No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3430.13364556261664765No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3420.13325592540785275No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3360.1309181021550834No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3350.1305284649462885No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3240.1262424556495447No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT3230.12585281844074983TruSeq Adapter, Index 12 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3220.12546318123195493No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3180.12390463239677536No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3160.12312535797918558No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3140.12234608356159579No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.12117717193521113No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3080.12000826030882646No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3060.11922898589123666No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2990.11650152542967242No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2960.11533261380328776No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2930.11416370217690308No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2850.11104660450654395No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2830.11026733008895417No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2830.11026733008895417No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2800.10909841846256951No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2780.10831914404497972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2760.10753986962738994No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2750.10715023241859505No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2740.10676059520980015No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2680.10442277195703081No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2670.10403313474823592No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2640.10286422312185124No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2600.10130567428667167No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC2590.1009160370778768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGT200.001839273137.04
AGAAGTC200.001839273137.05
TCGACGA200.001839273137.010
TTACTTT251.2309548E-437.03
AACGAAG200.001839273137.019
GTCGACG200.001839273137.09
CGAAGAG200.001839273137.021
TGATACA403.8135113E-837.02
ATTACTT200.001839273137.02
AGAGCGA200.001839273137.024
CGAACAA200.001839273137.014
GACGATG200.001839273137.029
GACGAAC200.001839273137.012
GAACAAC200.001839273137.015
TCGTATT200.001839273137.037
CGACGAA200.001839273137.011
CACTAGA251.2309548E-437.01
ACTAGAA200.001839273137.02
ACGATGC200.001839273137.030
GATTACG403.8135113E-837.01
Produced by FastQC (version 0.11.5)