FastQCFastQC Report
Fri 10 Feb 2017
ERR1631127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436108
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT172483.9549836279086836No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG143233.2842782063158666No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT131833.0228750676437945RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC80091.8364716996707238No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC54591.251754152641089RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC41290.9467838241903382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.800719087932347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33480.7676997441000852No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG18030.41342970089977715No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16570.3799517550698451No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA14430.3308813413191228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13460.30863914443211315No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT12620.28937786052996045No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT10360.23755583479321635No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9660.2215047648747558No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8600.19719885899822978No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7640.1751859631100553No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7390.16945343813917654No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7050.1616572041787814No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6740.15454887321489172No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6700.15363166921955113No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6290.14423032826730994No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6260.1435424252708045No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4980.11419189741990515No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4880.11189888743155364No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4820.11052308143854274No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAAT4790.10983517844203729No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4660.10685426545718033No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4580.10501985746649911No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4460.1022682454804773No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4390.10066313848863125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG403.8240614E-837.01
CGTCTTC7900.034.4240537
CTTATAC19500.034.153851
TTATACA19850.033.178842
CGATGAT352.3849345E-531.71428730
AGCAACG303.595849E-430.8333322
GCAACGC303.595849E-430.8333323
CCTACCG303.595849E-430.83333222
TTCCATC303.595849E-430.8333328
CTACCGA303.595849E-430.83333223
CTCCTCT303.595849E-430.83333219
TACCGAG303.595849E-430.83333224
ACAGACA551.8500941E-830.27272817
CAGACAC551.8500941E-830.27272818
TATACAC22200.030.03
GACCGCT250.005492650429.59999837
GGTATCA3800.029.2105271
TAGGACC1150.028.9565224
CCGGGGC454.0007617E-628.77777734
GTTGTAT454.0007617E-628.7777771
Produced by FastQC (version 0.11.5)