FastQCFastQC Report
Fri 10 Feb 2017
ERR1631126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278277
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT161895.81758463689058No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG136634.909856006784607No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT112374.038062793547437TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC75082.6980310985097584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44761.6084692590476395No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC42351.5218649043938235RNA PCR Primer, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40541.4568217998612893No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC37561.349734257592255TruSeq Adapter, Index 11 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25440.9141970051423581No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG18070.6493529828192772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15500.5569989614664526No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14830.5329222321643542No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA13060.4693165443065722No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12700.45637979423380304No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11060.3974457105689655No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT10960.39385216888208513No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10930.392774106376021No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10510.37768123129112363No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10360.3722909187608031No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9570.3439019394344484No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8920.32054391846972624No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT8880.3191065017949741No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8150.29287364748074757No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7590.2727498140342177No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6970.2504698555755596No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6550.23537698049066216No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6470.2325021471411579No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6180.22208087624920494No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6100.21920604289970066No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6060.21776862622494855No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6020.21633120955019636No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5810.20878477200774767No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5560.19980091779054684No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5300.19045770940465795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.18830158439252975No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5150.18506739687433743No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5070.18219256352483315No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5060.18183320935614514No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4740.17033387595812804No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4730.16997452178944No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4690.1685371051146879No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4660.1674590426086238No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4510.16206873007830327No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4230.15200681335503832No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4130.148413271668158No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4040.14517908414996566No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3900.14014812578833322No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3850.13835135494489303No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.13691393827014092No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3810.13691393827014092No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3680.13224233407719646No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3660.13152362573982038No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT3650.13116427157113236TruSeq Adapter, Index 11 (95% over 22bp)
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3600.12936750072769218No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3560.12793008405294007No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3530.12685202154687597No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3510.1261333132094999No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3510.1261333132094999No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3490.12541460487212383No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3350.12038364651049134No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3320.11930558400442724No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3290.11822752149836313No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3210.11535268814885886No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3100.1113997922932905No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3030.10888431311247428No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2950.10600947976296998No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2900.10421270891952981No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC2890.10385335475084179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2880.10349400058215374No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2850.10241593807608965No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2850.10241593807608965No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2830.10169722973871359No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2790.10025981306396145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGAC403.815512E-837.016
AATCATT200.001839539537.025
CGAAAGT355.610873E-737.020
ATTACTT200.001839539537.02
TAAACAT251.2312221E-437.02
TTGTATC251.2312221E-437.02
TATTGTA200.001839539537.05
TAGCAAC200.001839539537.01
ACTCAGA551.2732926E-1137.01
CGCCGAG200.001839539537.02
ATCATTG200.001839539537.026
CGTTTAT200.001839539537.01
CTTATAC17100.035.5935671
TCAGAGT603.45608E-1133.9166683
TTATACA18300.033.259562
CGTCTTC5250.033.1238137
AGACAGA451.0749682E-732.8888915
ACAGACA451.0749682E-732.8888917
TTTACAC401.5943024E-632.3752
GACGTAT352.3818548E-531.71428528
Produced by FastQC (version 0.11.5)