FastQCFastQC Report
Fri 10 Feb 2017
ERR1631125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561567
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT120202.1404391639822142No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG116412.072949443254322No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT101751.8118942174308674TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC62601.1147378674316688No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA37180.6620759410720359No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC36780.654953015401546RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC29200.5199735739457625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.38962403417579733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19900.3543655521068724No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG14190.2526857881606291No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA10860.1933874319538007No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA10470.18644257942507306No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC10030.17860736118753417No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT9490.1689914115323728No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG8420.14993758536381233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8010.1426365865515602No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7870.14014356256688873No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA7580.13497944145578353No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC7050.12554156494238444No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG6950.12376083352476197No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT6330.11272029873550261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15150.033.9471971
GATTACG401.5981386E-632.3751
TTATACA16550.031.1873112
CGTCTTC4800.030.44791837
TATACAC17550.030.0427363
GCCGCTA250.00549397929.633
GGTATCA4050.029.2345681
TTACGGG604.314461E-827.7500023
GCTTTAT5500.025.2272741
CCGTCTT15000.024.91333237
GCCGTCT15700.023.92038236
GTATCAA11150.023.394621
TAGGACG400.001929963723.1250024
GTACGTG502.6997176E-422.224
ATTGAGC6350.021.8503936
TTATTGA6700.021.813434
GATACAC855.1688403E-821.7647063
TTTATTG6850.021.0656953
TGCCGTC18300.020.52185835
CTTTATT6950.020.4964052
Produced by FastQC (version 0.11.5)