##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631125.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561567
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.156576152088707	31.0	31.0	34.0	28.0	34.0
2	31.138177635081835	31.0	31.0	34.0	28.0	34.0
3	30.598039058562915	31.0	31.0	34.0	26.0	34.0
4	34.80801222294045	37.0	35.0	37.0	32.0	37.0
5	34.97110941348049	35.0	35.0	37.0	32.0	37.0
6	35.17452236331551	37.0	35.0	37.0	32.0	37.0
7	35.18151529559251	37.0	35.0	37.0	32.0	37.0
8	35.219592675495534	37.0	35.0	37.0	32.0	37.0
9	36.946428476032246	39.0	37.0	39.0	33.0	39.0
10	36.73516962357119	38.0	37.0	39.0	32.0	39.0
11	36.80142173596383	39.0	37.0	39.0	33.0	39.0
12	36.724730976001084	38.0	36.0	39.0	32.0	39.0
13	36.83097119310786	39.0	37.0	39.0	33.0	39.0
14	37.85360250869442	39.0	37.0	41.0	33.0	41.0
15	37.85115400299519	39.0	37.0	41.0	33.0	41.0
16	37.838147540720875	39.0	37.0	41.0	33.0	41.0
17	37.815371985889485	39.0	37.0	41.0	33.0	41.0
18	37.86140567376644	39.0	37.0	41.0	33.0	41.0
19	37.864399083279466	40.0	37.0	41.0	33.0	41.0
20	37.86361734218713	40.0	37.0	41.0	33.0	41.0
21	37.793214344860004	39.0	37.0	41.0	33.0	41.0
22	37.70146928149268	39.0	37.0	40.0	32.0	41.0
23	37.752875436056605	39.0	37.0	41.0	33.0	41.0
24	37.68854829432641	39.0	37.0	41.0	32.0	41.0
25	37.657486996208824	39.0	37.0	41.0	32.0	41.0
26	37.51412921343312	39.0	37.0	40.0	32.0	41.0
27	37.39215445352024	39.0	36.0	40.0	32.0	41.0
28	37.36862921076203	39.0	36.0	40.0	32.0	41.0
29	37.3060365014326	39.0	36.0	40.0	32.0	41.0
30	37.24729907562232	39.0	36.0	40.0	31.0	41.0
31	37.198993174456476	39.0	36.0	40.0	31.0	41.0
32	37.11010084282018	39.0	36.0	40.0	31.0	41.0
33	37.088781926288405	39.0	36.0	40.0	31.0	41.0
34	37.037130030788845	39.0	36.0	40.0	31.0	41.0
35	37.02002254405975	39.0	36.0	40.0	31.0	41.0
36	37.00705169641378	39.0	36.0	40.0	31.0	41.0
37	36.91550607496523	39.0	36.0	40.0	31.0	41.0
38	36.87067616152658	39.0	36.0	40.0	31.0	41.0
39	36.76579464249146	39.0	35.0	40.0	30.0	41.0
40	36.64232228745635	39.0	35.0	40.0	30.0	41.0
41	36.62826875510847	39.0	35.0	40.0	30.0	41.0
42	36.57537568981083	39.0	35.0	40.0	30.0	41.0
43	35.81769584038948	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	9.0
10	7.0
11	9.0
12	5.0
13	1.0
14	11.0
15	8.0
16	13.0
17	16.0
18	15.0
19	34.0
20	69.0
21	130.0
22	296.0
23	596.0
24	1007.0
25	1596.0
26	2628.0
27	4121.0
28	6119.0
29	8414.0
30	11316.0
31	15017.0
32	19549.0
33	25014.0
34	32939.0
35	44153.0
36	61248.0
37	90078.0
38	141659.0
39	95489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.510038873366845	22.835921626448847	15.943956820824587	23.71008267935972
2	13.915703736152588	21.88305224487906	40.023363196199206	24.177880822769144
3	15.101136640863869	28.629709366825328	34.67671711478773	21.592436877523074
4	11.4383501879562	19.195572389403225	37.64822363137435	31.717853791266226
5	12.527801669257633	38.10622775198685	35.58631472290929	13.77965585584623
6	27.483986772727032	40.40408357328689	17.018094011934462	15.093835642051618
7	24.059996402922536	33.422369904214456	22.330728123269353	20.186905569593655
8	25.618314466483966	33.446943997777645	22.916410686525385	18.018330849213005
9	23.592554405796637	14.614462744427648	18.935941748713866	42.857041101061846
10	14.466127817339695	29.10000053421943	32.8981225748664	23.53574907357448
11	31.217824409197835	22.88845320326871	24.82036871824733	21.073353669286124
12	20.730562871393797	26.931960033264062	27.514615353110138	24.822861742232007
13	30.005324386938693	19.83806028488141	25.944900608475923	24.211714719703973
14	21.777276798672286	22.44148961744547	26.50992668728754	29.271306896594705
15	24.452825753650053	29.205775980426203	22.002717396143293	24.338680869780454
16	25.08801265031599	26.78380317931787	23.379222781965463	24.748961388400673
17	21.42024014943898	30.904949899121565	23.934276764838387	23.740533186601066
18	23.9016893798959	24.63463843138931	25.79371651111978	25.669955677595013
19	25.007701663381216	26.575991822881328	25.144283763112863	23.27202275062459
20	25.02764585525859	25.239552893955665	24.997017274875482	24.735783975910266
21	24.894803291503955	25.12594222951135	26.806240395179913	23.173014083804784
22	21.088846032619436	32.5462500467442	25.60602741970237	20.758876500933994
23	23.504586273766087	24.306271557979723	26.289472137785875	25.899670030468315
24	22.602111591315015	28.030849391078895	27.368061157439804	21.998977860166285
25	22.879371473038837	29.93872503191961	24.92258270161886	22.25932079342269
26	25.47460944108183	25.756677297633228	26.533254268858393	22.235458992426548
27	23.193848641390964	29.952080517551778	25.859247427288285	20.99482341376897
28	21.46458036173778	24.49502908824771	29.50066510318448	24.53972544683003
29	21.230236107178662	28.13751520299448	26.78487161816845	23.847377071658414
30	24.278670221006575	25.717857352729062	26.892427795792845	23.11104463047152
31	22.466241784150423	24.940033869511563	29.779883789467686	22.81384055687033
32	21.83871203258026	30.976535302109987	26.639385861348693	20.54536680396106
33	21.82179508411285	26.020581693724882	28.846958599775274	23.310664622386998
34	22.141792519859607	28.040643413875816	28.430801667476903	21.386762398787678
35	21.296835462197745	27.040762722880796	30.541146470501296	21.121255344420167
36	24.947512941465575	25.456624053763843	25.51289516656071	24.082967838209864
37	22.13645032560674	26.455258232766525	28.755607078051238	22.652684363575496
38	22.433120179782644	24.17271670165804	28.767181832265788	24.626981286293532
39	25.30081005472188	25.189158194836946	26.334346569509957	23.175685180931215
40	21.04931379514822	23.986274122232967	32.8320574392726	22.132354643346208
41	22.206433070319303	25.709309841924473	25.986035504223004	26.09822158353322
42	22.46374876016575	24.309476874531445	30.52796905801089	22.698805307291916
43	23.13971440629524	24.69500522644671	29.692627950004187	22.47265241725386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	21.5
2	30.0
3	99.5
4	169.0
5	169.0
6	219.0
7	269.0
8	250.0
9	231.0
10	349.5
11	468.0
12	468.0
13	725.0
14	982.0
15	2266.0
16	3550.0
17	3952.0
18	4354.0
19	4354.0
20	4665.0
21	4976.0
22	6003.5
23	7031.0
24	8757.5
25	10484.0
26	10484.0
27	12601.5
28	14719.0
29	17034.5
30	19350.0
31	21567.5
32	23785.0
33	23785.0
34	25742.0
35	27699.0
36	29232.0
37	30765.0
38	31805.5
39	32846.0
40	32846.0
41	33315.5
42	33785.0
43	33858.0
44	33931.0
45	34648.0
46	35365.0
47	35365.0
48	37242.5
49	39120.0
50	48774.5
51	58429.0
52	45714.0
53	32999.0
54	32999.0
55	40399.0
56	47799.0
57	39460.0
58	31121.0
59	25428.0
60	19735.0
61	19735.0
62	18009.0
63	16283.0
64	14435.0
65	12587.0
66	10268.0
67	7949.0
68	7949.0
69	6601.0
70	5253.0
71	4546.5
72	3840.0
73	2419.0
74	998.0
75	998.0
76	664.5
77	331.0
78	246.5
79	162.0
80	123.5
81	85.0
82	85.0
83	62.0
84	39.0
85	31.0
86	23.0
87	15.5
88	8.0
89	8.0
90	5.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	561567.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.0426289820468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.05865210238883	44.586918961979585
2	9.76675375296868	10.361085913554618
3	2.4149813773600948	3.842908835935916
4	1.0017540822275315	2.12542680459383
5	0.5105812796922903	1.3541286691948409
6	0.362917172151774	1.1550048548196092
7	0.24546192418450158	0.9113962041616472
8	0.19681523419410393	0.8351677956298005
9	0.15532066775421996	0.7414754897637764
>10	1.0941325615802793	11.244627699499826
>50	0.11551055439324404	4.316575966519848
>100	0.06937368544779746	6.667158323267601
>500	0.0033676546333882263	1.293097178816155
>1k	0.0030308891700494035	3.4027086834188336
>5k	3.367654633388226E-4	1.1182191379181006
>10k+	0.0010102963900164678	6.044099480926015
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	12020	2.1404391639822142	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	11641	2.072949443254322	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	10175	1.8118942174308674	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	6260	1.1147378674316688	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3718	0.6620759410720359	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	3678	0.654953015401546	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	2920	0.5199735739457625	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2188	0.38962403417579733	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1990	0.3543655521068724	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1419	0.2526857881606291	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1086	0.1933874319538007	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1047	0.18644257942507306	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1003	0.17860736118753417	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	949	0.1689914115323728	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	842	0.14993758536381233	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.1426365865515602	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	787	0.14014356256688873	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	758	0.13497944145578353	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	705	0.12554156494238444	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	695	0.12376083352476197	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	633	0.11272029873550261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.7807314176224742E-4	0.0	0.0	0.0	0.0
6	1.7807314176224742E-4	0.0	0.0	0.0	0.0
7	1.7807314176224742E-4	0.0	0.0	0.0	0.0
8	1.7807314176224742E-4	0.0	0.0	0.0	0.0
9	1.7807314176224742E-4	0.0	0.0	0.0	0.0
10	1.7807314176224742E-4	0.0	0.0	0.0	0.0
11	1.7807314176224742E-4	0.0	0.0	0.0	0.0
12	1.7807314176224742E-4	0.0	0.0	0.0	0.0
13	1.7807314176224742E-4	0.0	0.0	0.0	0.0
14	1.7807314176224742E-4	0.0	0.0	1.7807314176224742E-4	0.0
15	3.5614628352449484E-4	0.0	0.0	1.7807314176224742E-4	0.0
16	3.5614628352449484E-4	0.0	0.0	1.7807314176224742E-4	0.0
17	3.5614628352449484E-4	0.0	0.0	3.5614628352449484E-4	0.0
18	3.5614628352449484E-4	0.0	0.0	3.5614628352449484E-4	0.0
19	3.5614628352449484E-4	0.0	0.0	3.5614628352449484E-4	0.0
20	3.5614628352449484E-4	0.0	0.0	7.122925670489897E-4	0.0
21	3.5614628352449484E-4	0.0	0.0	0.0012465119923357319	0.0
22	3.5614628352449484E-4	0.0	0.0	0.0014245851340979794	0.0
23	3.5614628352449484E-4	0.0	0.0	0.0023149508429092165	0.0
24	3.5614628352449484E-4	0.0	0.0	0.0028491702681959588	0.0
25	3.5614628352449484E-4	0.0	0.0	0.0028491702681959588	0.0
26	3.5614628352449484E-4	0.0	0.0	0.003027243409958206	0.0
27	3.5614628352449484E-4	0.0	0.0	0.003383389693482701	0.0
28	3.5614628352449484E-4	0.0	0.0	0.00836943766282563	0.0
29	3.5614628352449484E-4	0.0	0.0	0.019588045593847216	0.0
30	3.5614628352449484E-4	0.0	0.0	0.03508040892716274	0.0
31	3.5614628352449484E-4	0.0	0.0	0.06677742816084278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1515	0.0	33.947197	1
GATTACG	40	1.5981386E-6	32.375	1
TTATACA	1655	0.0	31.187311	2
CGTCTTC	480	0.0	30.447918	37
TATACAC	1755	0.0	30.042736	3
GCCGCTA	25	0.005493979	29.6	33
GGTATCA	405	0.0	29.234568	1
TTACGGG	60	4.314461E-8	27.750002	3
GCTTTAT	550	0.0	25.227274	1
CCGTCTT	1500	0.0	24.913332	37
GCCGTCT	1570	0.0	23.920382	36
GTATCAA	1115	0.0	23.39462	1
TAGGACG	40	0.0019299637	23.125002	4
GTACGTG	50	2.6997176E-4	22.2	24
ATTGAGC	635	0.0	21.850393	6
TTATTGA	670	0.0	21.81343	4
GATACAC	85	5.1688403E-8	21.764706	3
TTTATTG	685	0.0	21.065695	3
TGCCGTC	1830	0.0	20.521858	35
CTTTATT	695	0.0	20.496405	2
>>END_MODULE