FastQCFastQC Report
Fri 10 Feb 2017
ERR1631124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598151
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61851.0340198378001542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58280.9743359118349714No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG34320.5737681622199077No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT34290.5732666166235616No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23180.3875275641100659No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT22950.38368238120474596No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC22080.3691375589107098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18960.3169768168907182No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15500.2591318914454711No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11890.19877923801849365No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11860.19827769242214757No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC11820.19760896496035285No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9790.16367104627426854No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9660.16149768202343553No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8940.1494605877111298No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8780.14678567786395075No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8510.1422717674968361No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7940.13274240116626068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.12973312758818425No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7100.11869912446857064No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC6990.11686012394863504Illumina PCR Primer Index 7 (95% over 21bp)
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6790.11351648663966121No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6310.10549175709812404No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6160.10298402911639369No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAAT6140.1026496653854963No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGA6020.10064348300011201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG850.036.9999961
CGTCTTC850.034.8235337
GCTCTAG352.3864068E-531.7142871
CTCGCTA352.3864068E-531.71428713
GGTATCA5750.030.8869551
ACACGAA303.597433E-430.83333217
ACCTCAC250.005494261629.622
GCACACT250.005494261629.66
CGTAGCT250.005494261629.625
GTGCGGT250.005494261629.613
GCGTAGC250.005494261629.624
TGCACAC250.005494261629.65
AGCCTCG405.9354414E-527.7510
CCGTCTT3250.027.32307637
TCAGAGT950.027.2631593
TTATACA4150.026.7469882
GACGTAT358.8644004E-426.42857228
ACGTATA358.8644004E-426.42857229
GTATCAA19750.025.6658211
GCCGTCT3400.025.57352836
Produced by FastQC (version 0.11.5)