##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631124.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	598151
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49082422331485	31.0	31.0	34.0	28.0	34.0
2	31.4574212865982	31.0	31.0	34.0	28.0	34.0
3	30.90798310125704	31.0	31.0	34.0	27.0	34.0
4	35.09478710225344	37.0	35.0	37.0	32.0	37.0
5	35.315179611837145	37.0	35.0	37.0	33.0	37.0
6	35.52942317240964	37.0	35.0	37.0	33.0	37.0
7	35.54660278090315	37.0	35.0	37.0	33.0	37.0
8	35.56909041362465	37.0	35.0	37.0	33.0	37.0
9	37.33504583290841	39.0	37.0	39.0	34.0	39.0
10	37.17123101022986	39.0	37.0	39.0	33.0	39.0
11	37.239013225757375	39.0	37.0	39.0	34.0	39.0
12	37.17021120085062	39.0	37.0	39.0	33.0	39.0
13	37.24996196612561	39.0	37.0	39.0	34.0	39.0
14	38.34020840891347	40.0	38.0	41.0	34.0	41.0
15	38.34314746610806	40.0	38.0	41.0	34.0	41.0
16	38.370424859274664	40.0	38.0	41.0	34.0	41.0
17	38.37957137913336	40.0	38.0	41.0	34.0	41.0
18	38.41475480271704	40.0	38.0	41.0	34.0	41.0
19	38.433826909927426	40.0	38.0	41.0	34.0	41.0
20	38.3955339036464	40.0	38.0	41.0	34.0	41.0
21	38.35729105192502	40.0	38.0	41.0	34.0	41.0
22	38.287118135721585	40.0	38.0	41.0	34.0	41.0
23	38.25852000581793	40.0	38.0	41.0	34.0	41.0
24	38.20355729573302	40.0	38.0	41.0	34.0	41.0
25	38.16747610553188	40.0	37.0	41.0	33.0	41.0
26	38.04426139887754	40.0	37.0	41.0	33.0	41.0
27	37.949029592862004	40.0	37.0	41.0	33.0	41.0
28	37.894827560264886	40.0	37.0	41.0	33.0	41.0
29	37.79580072590366	40.0	37.0	41.0	33.0	41.0
30	37.64093849211988	39.0	37.0	41.0	33.0	41.0
31	37.54437424663672	39.0	36.0	41.0	33.0	41.0
32	37.40005450128814	39.0	36.0	41.0	32.0	41.0
33	37.3768446429079	39.0	36.0	41.0	32.0	41.0
34	37.313701724146576	39.0	36.0	41.0	32.0	41.0
35	37.193636723837294	39.0	36.0	41.0	31.0	41.0
36	37.13466499261892	39.0	36.0	41.0	31.0	41.0
37	37.04084921700373	39.0	35.0	41.0	31.0	41.0
38	36.9690011385085	39.0	35.0	41.0	31.0	41.0
39	36.847366300482655	39.0	35.0	41.0	31.0	41.0
40	36.74372357481639	39.0	35.0	41.0	31.0	41.0
41	36.618758474030805	39.0	35.0	41.0	30.0	41.0
42	36.47345402749473	39.0	35.0	40.0	30.0	41.0
43	35.8274666430383	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	4.0
12	6.0
13	4.0
14	4.0
15	0.0
16	1.0
17	9.0
18	12.0
19	27.0
20	65.0
21	130.0
22	262.0
23	485.0
24	863.0
25	1523.0
26	2404.0
27	3666.0
28	5253.0
29	7233.0
30	10420.0
31	13570.0
32	17766.0
33	23165.0
34	31046.0
35	42179.0
36	58563.0
37	88416.0
38	142159.0
39	148909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.164272900989886	21.59638619679646	15.419350632198226	22.81999027001543
2	15.239462944975433	22.905253021394266	39.51828217289614	22.337001860734162
3	15.233110034088382	29.726941859162654	31.784783440970592	23.255164665778373
4	11.830624708476623	18.38682874391249	39.77590942755258	30.00663712005831
5	12.947733933404775	38.71614358247332	34.295855060009934	14.04026742411197
6	27.047183737885582	41.10433653040788	17.254338787363057	14.594140944343485
7	25.91703432745243	31.243281378782278	23.699366882275548	19.14031741148974
8	25.74600727909842	32.97127314005996	21.369186041651687	19.913533539189938
9	25.863034584912505	14.013351143774733	19.976226738733196	40.147387532579565
10	16.588286235415474	27.16788904473954	32.581739393564504	23.662085326280486
11	33.07693207902352	24.39116544150223	23.584512940712298	18.947389538761954
12	22.313763581436792	27.496735774077113	27.9715322719514	22.217968372534695
13	29.896129906996727	21.285595108927343	25.781449834573543	23.036825149502384
14	22.917958843168364	21.214208452380753	28.284831087802242	27.583001616648637
15	24.7079750765275	28.158107233792133	24.246051582292765	22.8878661073876
16	22.557347559395538	25.966520159625244	25.188622939692486	26.28750934128673
17	22.228333648192514	27.056211558619815	25.816892390048668	24.898562403139007
18	23.3603220591456	24.93266750369054	28.19221233434367	23.51479810282019
19	25.204003671313764	24.662000063529106	29.10920486632974	21.024791398827386
20	24.060814075375617	24.322620876668267	28.63791918762988	22.97864586032624
21	23.925062400631276	25.360987442970085	28.464885956890484	22.24906419950815
22	22.144241169871822	26.85960568485215	28.446161587960226	22.549991557315796
23	23.209022470914533	24.842054932617348	29.146987967921145	22.80193462854697
24	22.021195316901583	26.848237318001644	28.068999299507986	23.061568065588787
25	22.592121387408863	26.16546657950919	29.22522908095113	22.017182952130817
26	23.415993620340014	26.03305854207382	28.802760506962287	21.74818733062387
27	21.883604641637312	26.639594349921676	28.60047044976937	22.87633055867164
28	21.90834755772372	25.62296142612818	29.96734938167787	22.501341634470226
29	23.766072446589572	25.98173370938108	28.466056229948627	21.786137614080726
30	22.086563426292024	25.692509082154842	28.965094098312967	23.25583339324017
31	25.214870492567933	24.715999806069036	27.487373589611984	22.581756111751048
32	21.093837509257696	26.20308249923514	29.522311255853456	23.180768735653707
33	21.6741257642301	25.425185279302383	29.19480198143947	23.705886975028044
34	22.515384911167917	25.12225173910936	29.001539744980782	23.360823604741945
35	22.119665435650866	26.019683992837926	30.331973030221466	21.528677541289742
36	23.55977002462589	26.162290124065663	26.77985993503313	23.498079916275323
37	22.908596658703235	25.42418218810969	28.719671119834288	22.947550033352783
38	22.734393154905703	25.697524538118298	27.98758173103447	23.580500575941528
39	22.425942613152866	25.476510111995132	29.13712423785967	22.96042303699233
40	21.975554667634093	25.81739393564501	29.95698410602005	22.25006729070084
41	23.188626283329793	24.742247358944482	27.59286534671011	24.476261011015612
42	21.895976099680514	26.048773637426002	28.730705122953903	23.32454513993958
43	23.364835969512715	24.616359414261616	28.947874366171757	23.070930250053916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	54.0
2	42.0
3	146.0
4	250.0
5	250.0
6	325.0
7	400.0
8	422.5
9	445.0
10	754.0
11	1063.0
12	1063.0
13	1586.0
14	2109.0
15	5562.0
16	9015.0
17	8976.0
18	8937.0
19	8937.0
20	7012.5
21	5088.0
22	4150.5
23	3213.0
24	3650.0
25	4087.0
26	4087.0
27	5294.5
28	6502.0
29	8333.0
30	10164.0
31	12731.5
32	15299.0
33	15299.0
34	19423.0
35	23547.0
36	26370.5
37	29194.0
38	33892.0
39	38590.0
40	38590.0
41	41750.0
42	44910.0
43	48249.0
44	51588.0
45	54236.5
46	56885.0
47	56885.0
48	58035.5
49	59186.0
50	56279.5
51	53373.0
52	53705.5
53	54038.0
54	54038.0
55	48277.5
56	42517.0
57	37462.0
58	32407.0
59	26220.0
60	20033.0
61	20033.0
62	16041.5
63	12050.0
64	8935.5
65	5821.0
66	4732.5
67	3644.0
68	3644.0
69	2750.0
70	1856.0
71	1425.0
72	994.0
73	648.0
74	302.0
75	302.0
76	240.0
77	178.0
78	139.0
79	100.0
80	85.0
81	70.0
82	70.0
83	61.5
84	53.0
85	58.0
86	63.0
87	52.0
88	41.0
89	41.0
90	35.5
91	30.0
92	15.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	598151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.59509771088534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.37826699015692	12.52905458272088
2	11.490021704608484	4.273161525947762
3	4.690715747862037	2.6167295299644957
4	2.7016581231554757	2.009503871259327
5	1.760957799399747	1.6372591172291961
6	1.334973759221205	1.4894380496511734
7	1.0543517375438307	1.372404150688849
8	0.8463868975334082	1.259091764867746
9	0.6938192429263198	1.1611472954316668
>10	6.239832403671159	24.793432764819805
>50	1.0470858332286122	13.65209289105627
>100	0.7246960059986406	24.46954089480772
>500	0.02633607039343431	3.215445805979447
>1k	0.009081403583942863	3.4930674069998875
>5k	0.001816280716788573	2.0286303485757626
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6185	1.0340198378001542	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5828	0.9743359118349714	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3432	0.5737681622199077	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	3429	0.5732666166235616	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2318	0.3875275641100659	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2295	0.38368238120474596	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	2208	0.3691375589107098	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1896	0.3169768168907182	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1550	0.2591318914454711	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1189	0.19877923801849365	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1186	0.19827769242214757	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	1182	0.19760896496035285	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	979	0.16367104627426854	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	966	0.16149768202343553	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	894	0.1494605877111298	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	878	0.14678567786395075	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	851	0.1422717674968361	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	794	0.13274240116626068	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.12973312758818425	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	710	0.11869912446857064	No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC	699	0.11686012394863504	Illumina PCR Primer Index 7 (95% over 21bp)
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	679	0.11351648663966121	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	631	0.10549175709812404	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	616	0.10298402911639369	No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAAT	614	0.1026496653854963	No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGA	602	0.10064348300011201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.6718186544869106E-4	0.0
11	0.0	0.0	0.0	3.343637308973821E-4	0.0
12	0.0	0.0	0.0	3.343637308973821E-4	0.0
13	0.0	0.0	0.0	5.015455963460732E-4	0.0
14	0.0	0.0	0.0	5.015455963460732E-4	0.0
15	0.0	0.0	0.0	5.015455963460732E-4	0.0
16	0.0	0.0	0.0	5.015455963460732E-4	0.0
17	0.0	0.0	0.0	6.687274617947642E-4	0.0
18	0.0	0.0	0.0	6.687274617947642E-4	0.0
19	0.0	0.0	0.0	6.687274617947642E-4	0.0
20	0.0	0.0	0.0	0.0011702730581408373	0.0
21	0.0	0.0	0.0	0.0016718186544869105	0.0
22	0.0	0.0	0.0	0.002173364250832984	0.0
23	0.0	0.0	0.0	0.003343637308973821	0.0
24	0.0	0.0	0.0	0.004346728501665968	0.0
25	0.0	0.0	0.0	0.004848274098012041	0.0
26	0.0	0.0	0.0	0.004848274098012041	0.0
27	0.0	0.0	0.0	0.007021638348845024	0.0
28	0.0	0.0	0.0	0.018390005199356015	0.0
29	0.0	0.0	0.0	0.04330010315121098	0.0
30	0.0	0.0	0.0	0.07121947468114238	0.0
31	0.0	0.0	0.0	0.14829031465298897	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	85	0.0	36.999996	1
CGTCTTC	85	0.0	34.82353	37
GCTCTAG	35	2.3864068E-5	31.714287	1
CTCGCTA	35	2.3864068E-5	31.714287	13
GGTATCA	575	0.0	30.886955	1
ACACGAA	30	3.597433E-4	30.833332	17
ACCTCAC	25	0.0054942616	29.6	22
GCACACT	25	0.0054942616	29.6	6
CGTAGCT	25	0.0054942616	29.6	25
GTGCGGT	25	0.0054942616	29.6	13
GCGTAGC	25	0.0054942616	29.6	24
TGCACAC	25	0.0054942616	29.6	5
AGCCTCG	40	5.9354414E-5	27.75	10
CCGTCTT	325	0.0	27.323076	37
TCAGAGT	95	0.0	27.263159	3
TTATACA	415	0.0	26.746988	2
GACGTAT	35	8.8644004E-4	26.428572	28
ACGTATA	35	8.8644004E-4	26.428572	29
GTATCAA	1975	0.0	25.665821	1
GCCGTCT	340	0.0	25.573528	36
>>END_MODULE