FastQCFastQC Report
Fri 10 Feb 2017
ERR1631123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270594
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT131494.859309519058072No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT111764.130172878925623TruSeq Adapter, Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG96273.557728552739529No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC48851.805287626481001No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC41341.5277500609769619RNA PCR Primer, Index 1 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35811.3233848496271166No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC34621.2794075256657576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33541.2394953324907425No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15510.5731834408745206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15020.5550751310080785No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA10620.39246989955431383No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG10390.3839700806374125No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT8460.3126455132042839No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8060.29786321943575983No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7330.2708855333082034No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7150.2642335011123676No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT6450.23836448701745047No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6310.23319068419846706No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6140.22690820934684436No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6050.22358219324892645No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5970.22062573449522163No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5860.21656060370887753No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5480.20251742462877964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.1688877063053874No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4380.1618661167653385No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3960.14634470830838822No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3940.145605593619962No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3920.14486647893153579No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3840.141910020177831No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT3720.13747533204727377RNA PCR Primer, Index 40 (95% over 22bp)
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3620.13377975860514277No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3540.13082329985143795No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3470.12823639844194623No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3440.12712772640930695No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3370.12454082499981523No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3250.120106136869258No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3190.11788879280397939No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3140.11604100608291387No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3140.11604100608291387No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2970.10975853123129115No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2860.10569340044494703No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2800.10347605637966843No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2790.10310649903545532No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2770.10236738434702913No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2750.10162826965860292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATATG200.001839449737.02
CTTATAC15850.035.365931
TTATACA16450.034.0759852
CGTCTTC5500.031.95454437
TATACAC17850.031.921573
TGATACA352.3816132E-531.7142852
GATACAC352.3816132E-531.7142853
TCAGAGT352.3816132E-531.7142853
TCGCCGA303.592271E-430.8333341
TAACAAG303.592271E-430.83333431
TCATGTT303.592271E-430.83333424
CATGTTA303.592271E-430.83333425
CGCCGAG303.592271E-430.8333342
CGGCGTA250.0054890129.612
AGCAACG250.0054890129.62
CTATAGA250.0054890129.63
TTACTTA250.0054890129.629
CCTAAGG250.0054890129.63
TCGGCGT250.0054890129.611
GTTACTT250.0054890129.628
Produced by FastQC (version 0.11.5)