##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270594
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29129248985565	31.0	31.0	34.0	28.0	34.0
2	31.288140165709514	31.0	31.0	34.0	28.0	34.0
3	30.742400053216258	31.0	31.0	34.0	26.0	34.0
4	34.90805413275978	37.0	35.0	37.0	32.0	37.0
5	35.11795161755249	35.0	35.0	37.0	32.0	37.0
6	35.32712476995055	37.0	35.0	37.0	33.0	37.0
7	35.31094185384746	37.0	35.0	37.0	33.0	37.0
8	35.35126056010111	37.0	35.0	37.0	33.0	37.0
9	37.13122611735663	39.0	37.0	39.0	33.0	39.0
10	36.96677679475524	39.0	37.0	39.0	33.0	39.0
11	37.03589140926998	39.0	37.0	39.0	33.0	39.0
12	36.916040266968224	39.0	37.0	39.0	33.0	39.0
13	37.024272526367916	39.0	37.0	39.0	33.0	39.0
14	37.936583959733035	40.0	37.0	41.0	33.0	41.0
15	37.935911365366564	40.0	37.0	41.0	33.0	41.0
16	37.94899369535171	40.0	37.0	41.0	33.0	41.0
17	37.944034235792365	40.0	37.0	41.0	33.0	41.0
18	38.05416232436787	40.0	37.0	41.0	33.0	41.0
19	38.00590922193397	40.0	37.0	41.0	33.0	41.0
20	38.087097274884144	40.0	37.0	41.0	33.0	41.0
21	38.03074347546509	40.0	37.0	41.0	33.0	41.0
22	37.890477985469005	40.0	37.0	41.0	33.0	41.0
23	37.88897019150462	40.0	37.0	41.0	33.0	41.0
24	37.73762537232903	39.0	37.0	41.0	33.0	41.0
25	37.71520802382906	39.0	37.0	41.0	32.0	41.0
26	37.50595356881527	39.0	36.0	41.0	32.0	41.0
27	37.33922407740009	39.0	36.0	41.0	32.0	41.0
28	37.423871187092104	39.0	36.0	41.0	32.0	41.0
29	37.28927840232969	39.0	36.0	41.0	32.0	41.0
30	37.16004419905837	39.0	36.0	40.0	31.0	41.0
31	37.06191563744947	39.0	35.0	40.0	31.0	41.0
32	36.805224801732486	39.0	35.0	40.0	31.0	41.0
33	36.63129263767859	39.0	35.0	40.0	30.0	41.0
34	36.47194320642734	38.0	35.0	40.0	30.0	41.0
35	36.417562843226385	39.0	35.0	40.0	30.0	41.0
36	36.33205466492235	39.0	35.0	40.0	30.0	41.0
37	36.1891246664745	38.0	35.0	40.0	30.0	41.0
38	36.10167631211335	38.0	35.0	40.0	30.0	41.0
39	35.94341707502753	38.0	35.0	40.0	29.0	41.0
40	35.64094177993599	38.0	35.0	40.0	27.0	41.0
41	35.57649097910523	38.0	35.0	40.0	27.0	41.0
42	35.39901476012033	38.0	34.0	40.0	26.0	41.0
43	34.535810106654246	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	4.0
10	15.0
11	16.0
12	14.0
13	12.0
14	9.0
15	7.0
16	10.0
17	13.0
18	12.0
19	32.0
20	47.0
21	124.0
22	193.0
23	424.0
24	628.0
25	1038.0
26	1687.0
27	2476.0
28	3519.0
29	4966.0
30	6325.0
31	7720.0
32	9799.0
33	12224.0
34	16083.0
35	20913.0
36	28281.0
37	41129.0
38	59804.0
39	53065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.182620457216345	21.444673569997857	17.369934292704198	25.002771680081597
2	12.884986363333997	21.68710318780165	42.02347428250442	23.404436166359936
3	14.288565156655359	30.016556169020745	36.09836138273576	19.596517291588135
4	9.75742255925852	20.42839087341183	32.42717872532281	37.387007842006845
5	13.017657449906503	37.06623206722987	32.79414916812642	17.12196131473721
6	26.43074125812102	38.802782027687236	15.709882702498948	19.056594011692795
7	22.965032484090557	33.48115627101858	18.937596546856174	24.616214698034693
8	27.61036830084924	30.828473654256932	22.676777755604338	18.88438028928949
9	21.801665964507713	12.421931011034983	17.04324560041982	48.733157424037486
10	15.862509885658957	27.44591528267441	29.204638683784562	27.48693614788207
11	29.47293731568327	21.110593730829212	24.26698300775331	25.149485945734202
12	19.316023267330394	27.969208482080166	23.60030155879288	29.114466691796565
13	32.26568216590168	17.627885318964946	25.89636133838888	24.210071176744496
14	23.188614677339483	23.79875385263531	24.506825724147614	28.505805745877588
15	26.682409809530146	26.591498702853723	20.98863980723889	25.737451680377244
16	28.270397717613843	23.30982948624138	20.94577115531017	27.47400164083461
17	19.0905932873604	33.04323081812605	21.75510173913686	26.11107415537669
18	25.55415123764754	21.076594455161608	24.284352202931327	29.08490210425952
19	23.168658580751973	28.375352003370363	26.30213530233486	22.1538541135428
20	20.622408479123706	20.558105501230624	29.17359586687066	29.645890152775006
21	24.815406106565554	21.59138783565046	26.343895282230946	27.24931077555304
22	18.62347280427504	30.929732366571322	30.307028241572244	20.139766587581395
23	22.47684723238505	22.001596487727003	25.499826308048217	30.02172997183973
24	24.591454355972417	30.583826692387856	25.91262186153425	18.912097090105473
25	24.805058500927586	26.164290412943377	26.97768612755641	22.05296495857262
26	28.033511459973244	27.70275763690252	24.763668078375723	19.500062824748515
27	19.92616244262622	29.993274056335324	32.07388190425509	18.006681596783373
28	18.441650590922194	21.775057835724372	30.13961876464371	29.643672808709727
29	19.49895415271588	28.425611802183344	29.675824297656266	22.39960974744451
30	19.8747939717806	21.087311618143787	35.799389491267355	23.23850491880825
31	23.580345462205372	23.402218822294653	24.433653369993422	28.583782345506553
32	24.805428058271804	22.078464415323324	33.432744258926654	19.683363267478214
33	21.68340761435952	22.185266487800913	31.415330716867334	24.715995180972232
34	19.14787467571343	33.03657878593022	26.726387133491503	21.08915940486485
35	25.070400674072594	22.023400371035574	35.182967841119904	17.723231113771924
36	28.588956148325533	22.571823469847814	24.18309349061694	24.65612689120971
37	20.777622563693207	25.719712927855014	29.202421339719287	24.30024316873249
38	20.88996799633399	21.138310531645196	30.332897255667163	27.63882421635365
39	26.05970568453107	21.51045477726779	27.202007435493762	25.227832102707374
40	18.35184815627841	21.592126950338884	38.56072196722766	21.495302926155052
41	22.533759063393866	22.887055884461592	25.383785301965307	29.195399750179234
42	23.00494467726557	21.42139145731243	34.36587655306474	21.207787312357258
43	23.03229192073734	20.644951477120703	33.111229369461256	23.211527232680695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	15.0
2	18.0
3	98.0
4	178.0
5	178.0
6	270.5
7	363.0
8	363.0
9	363.0
10	586.5
11	810.0
12	810.0
13	1193.0
14	1576.0
15	3566.5
16	5557.0
17	5599.5
18	5642.0
19	5642.0
20	4720.5
21	3799.0
22	2921.5
23	2044.0
24	2076.5
25	2109.0
26	2109.0
27	2320.0
28	2531.0
29	2916.0
30	3301.0
31	3794.5
32	4288.0
33	4288.0
34	5364.5
35	6441.0
36	7088.5
37	7736.0
38	9037.0
39	10338.0
40	10338.0
41	11302.5
42	12267.0
43	13520.5
44	14774.0
45	16908.0
46	19042.0
47	19042.0
48	21748.0
49	24454.0
50	34398.0
51	44342.0
52	32730.5
53	21119.0
54	21119.0
55	28629.5
56	36140.0
57	29199.5
58	22259.0
59	15179.5
60	8100.0
61	8100.0
62	6578.0
63	5056.0
64	3919.0
65	2782.0
66	2234.0
67	1686.0
68	1686.0
69	1294.5
70	903.0
71	626.0
72	349.0
73	224.5
74	100.0
75	100.0
76	74.5
77	49.0
78	34.0
79	19.0
80	18.0
81	17.0
82	17.0
83	12.5
84	8.0
85	7.0
86	6.0
87	7.5
88	9.0
89	9.0
90	7.5
91	6.0
92	3.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	270594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.61471429521719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.80438184663537	16.720252481577567
2	10.884194053208137	5.140542658004243
3	4.546165884194053	3.22069225481718
4	2.6776212832550863	2.529250463794467
5	1.8450704225352115	2.1785405441362338
6	1.350547730829421	1.9135679283354399
7	1.0093896713615023	1.6685514091221536
8	0.755868544600939	1.4279695780394244
9	0.621283255086072	1.3204283908734118
>10	4.735524256651017	21.83123055204476
>50	0.5086071987480438	8.233368071723689
>100	0.22535211267605634	9.48099366578712
>500	0.017214397496087636	2.707377103705182
>1k	0.014084507042253521	9.080023947315905
>5k	0.001564945226917058	3.557728552739529
>10k+	0.003129890453834116	8.989482397983695
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	13149	4.859309519058072	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	11176	4.130172878925623	TruSeq Adapter, Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	9627	3.557728552739529	No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	4885	1.805287626481001	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	4134	1.5277500609769619	RNA PCR Primer, Index 1 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3581	1.3233848496271166	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	3462	1.2794075256657576	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3354	1.2394953324907425	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1551	0.5731834408745206	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1502	0.5550751310080785	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1062	0.39246989955431383	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	1039	0.3839700806374125	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	846	0.3126455132042839	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	806	0.29786321943575983	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	733	0.2708855333082034	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	715	0.2642335011123676	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	645	0.23836448701745047	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	631	0.23319068419846706	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	614	0.22690820934684436	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	605	0.22358219324892645	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	597	0.22062573449522163	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	586	0.21656060370887753	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	548	0.20251742462877964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.1688877063053874	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	438	0.1618661167653385	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	396	0.14634470830838822	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	394	0.145605593619962	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	392	0.14486647893153579	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	384	0.141910020177831	No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT	372	0.13747533204727377	RNA PCR Primer, Index 40 (95% over 22bp)
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	362	0.13377975860514277	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	354	0.13082329985143795	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	347	0.12823639844194623	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	344	0.12712772640930695	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	337	0.12454082499981523	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	325	0.120106136869258	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	319	0.11788879280397939	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	314	0.11604100608291387	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	314	0.11604100608291387	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	297	0.10975853123129115	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	286	0.10569340044494703	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	280	0.10347605637966843	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	279	0.10310649903545532	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	277	0.10236738434702913	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	275	0.10162826965860292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.6955734421310156E-4	0.0
9	0.0	0.0	0.0	0.0011086720326393047	0.0
10	0.0	0.0	0.0	0.0011086720326393047	0.0
11	0.0	0.0	0.0	0.0014782293768524062	0.0
12	0.0	0.0	0.0	0.0014782293768524062	0.0
13	0.0	0.0	0.0	0.0014782293768524062	0.0
14	0.0	0.0	0.0	0.0014782293768524062	0.0
15	0.0	0.0	0.0	0.0018477867210655078	0.0
16	0.0	0.0	0.0	0.0018477867210655078	0.0
17	0.0	0.0	0.0	0.0018477867210655078	0.0
18	0.0	0.0	0.0	0.0018477867210655078	0.0
19	0.0	0.0	0.0	0.002586901409491711	0.0
20	0.0	0.0	0.0	0.004065130786344117	0.0
21	0.0	0.0	0.0	0.005912917507409625	0.0
22	0.0	0.0	0.0	0.006652032195835828	0.0
23	0.0	0.0	0.0	0.008869376261114437	0.0
24	0.0	0.0	0.0	0.011456277670606148	0.0
25	0.0	0.0	0.0	0.012564949703245452	0.0
26	0.0	0.0	0.0	0.013673621735884758	0.0
27	0.0	0.0	0.0	0.016260523145376467	0.0
28	0.0	0.0	0.0	0.029934144881261227	0.0
29	0.0	0.0	0.0	0.05875961772988315	0.0
30	0.0	0.0	0.0	0.10458472841230773	0.0
31	0.0	0.0	0.0	0.1655616902074695	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATATG	20	0.0018394497	37.0	2
CTTATAC	1585	0.0	35.36593	1
TTATACA	1645	0.0	34.075985	2
CGTCTTC	550	0.0	31.954544	37
TATACAC	1785	0.0	31.92157	3
TGATACA	35	2.3816132E-5	31.714285	2
GATACAC	35	2.3816132E-5	31.714285	3
TCAGAGT	35	2.3816132E-5	31.714285	3
TCGCCGA	30	3.592271E-4	30.833334	1
TAACAAG	30	3.592271E-4	30.833334	31
TCATGTT	30	3.592271E-4	30.833334	24
CATGTTA	30	3.592271E-4	30.833334	25
CGCCGAG	30	3.592271E-4	30.833334	2
CGGCGTA	25	0.00548901	29.6	12
AGCAACG	25	0.00548901	29.6	2
CTATAGA	25	0.00548901	29.6	3
TTACTTA	25	0.00548901	29.6	29
CCTAAGG	25	0.00548901	29.6	3
TCGGCGT	25	0.00548901	29.6	11
GTTACTT	25	0.00548901	29.6	28
>>END_MODULE