FastQCFastQC Report
Fri 10 Feb 2017
ERR1631122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398988
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT43950.3141556610921609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43610.31172533288348436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42550.30414842729172803No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG34390.24582055028349062No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT25990.18577714748089333RNA PCR Primer, Index 29 (95% over 23bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC25730.1839186612036701No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC20130.14388972600193856No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17590.12573374467829604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14450.1032889488687537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC200.001842072737.015
GATTACG950.033.1052631
GGTATCA7750.026.7354831
GTATCAA23900.024.5376571
TATACAC9800.023.4081633
CTTATAC9500.022.784211
CGAACGC903.8307917E-922.6111130
ACGAACG903.8307917E-922.6111129
CCGTCTT4850.021.36082537
TTATACA10700.021.2663562
GCCGTCT4950.020.92929336
GCAACGC1101.7553248E-920.1818183
CGTCTTC2250.019.73333437
AGCAACG951.6767626E-719.4736842
TACGGAA609.238183E-418.52
CGGCTAG701.2194373E-418.521
ACGGTAC2400.017.7291663
GCGGGTA852.7238015E-517.41176422
TTAGCGA1603.45608E-1117.3437527
CTAGCCG752.0676712E-417.26666613
Produced by FastQC (version 0.11.5)