##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631122.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1398988
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.526769350416156	31.0	31.0	34.0	28.0	34.0
2	31.502572573889125	31.0	31.0	34.0	28.0	34.0
3	30.96778385518675	31.0	31.0	34.0	27.0	34.0
4	35.13606764318207	37.0	35.0	37.0	32.0	37.0
5	35.33690210352054	37.0	35.0	37.0	33.0	37.0
6	35.527051697369814	37.0	35.0	37.0	33.0	37.0
7	35.54501468204159	37.0	35.0	37.0	33.0	37.0
8	35.567330813416554	37.0	35.0	37.0	33.0	37.0
9	37.33025587067223	39.0	37.0	39.0	34.0	39.0
10	37.17140961895313	39.0	37.0	39.0	33.0	39.0
11	37.23372394902601	39.0	37.0	39.0	34.0	39.0
12	37.17877637263508	39.0	37.0	39.0	33.0	39.0
13	37.24245669012171	39.0	37.0	39.0	34.0	39.0
14	38.42256902846915	40.0	38.0	41.0	34.0	41.0
15	38.42601080209408	40.0	38.0	41.0	34.0	41.0
16	38.43488007045092	40.0	38.0	41.0	34.0	41.0
17	38.415932802854634	40.0	38.0	41.0	34.0	41.0
18	38.42519235332969	40.0	38.0	41.0	34.0	41.0
19	38.46035348408993	40.0	38.0	41.0	34.0	41.0
20	38.437429770662796	40.0	38.0	41.0	34.0	41.0
21	38.40113281886621	40.0	38.0	41.0	34.0	41.0
22	38.36638770311111	40.0	38.0	41.0	34.0	41.0
23	38.329090742736895	40.0	38.0	41.0	34.0	41.0
24	38.311407960611525	40.0	38.0	41.0	34.0	41.0
25	38.293475712443566	40.0	38.0	41.0	34.0	41.0
26	38.18551624459967	40.0	38.0	41.0	34.0	41.0
27	38.13032706499269	40.0	38.0	41.0	33.0	41.0
28	38.07965186263213	40.0	37.0	41.0	33.0	41.0
29	38.0222732432301	40.0	37.0	41.0	33.0	41.0
30	37.93826323027788	40.0	37.0	41.0	33.0	41.0
31	37.90625223375754	40.0	37.0	41.0	33.0	41.0
32	37.84904087812047	40.0	37.0	41.0	33.0	41.0
33	37.82055886111961	40.0	37.0	41.0	33.0	41.0
34	37.75384134817454	40.0	37.0	41.0	33.0	41.0
35	37.69921471806763	40.0	37.0	41.0	33.0	41.0
36	37.668029318335826	40.0	37.0	41.0	33.0	41.0
37	37.60292154042779	40.0	37.0	41.0	32.0	41.0
38	37.56036506388904	40.0	37.0	41.0	32.0	41.0
39	37.49021292534318	40.0	37.0	41.0	32.0	41.0
40	37.44531475609512	40.0	37.0	41.0	32.0	41.0
41	37.36510749198707	39.0	36.0	41.0	32.0	41.0
42	37.305730999837024	39.0	36.0	41.0	31.0	41.0
43	36.73059239964889	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	3.0
11	5.0
12	5.0
13	7.0
14	4.0
15	4.0
16	5.0
17	9.0
18	16.0
19	57.0
20	120.0
21	224.0
22	422.0
23	859.0
24	1527.0
25	2831.0
26	4492.0
27	7075.0
28	10507.0
29	15391.0
30	21360.0
31	28420.0
32	38044.0
33	49981.0
34	66664.0
35	91027.0
36	126467.0
37	190280.0
38	337737.0
39	405435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17246752652632	21.1845991531021	14.77467998295911	23.868253337412472
2	14.688975173482547	22.176530463449293	40.21750007862826	22.916994284439895
3	15.309852550557975	27.970361432692776	32.62779952365567	24.09198649309358
4	12.551501513951512	18.02660208665121	39.94165782694348	29.480238572453803
5	12.999968548693769	38.31591121582172	35.39630075454543	13.287819480939078
6	28.65935947985258	40.04494677581223	17.89078962793105	13.404904116404143
7	26.083854900828314	32.08297712346353	24.055317129239135	17.77785084646902
8	25.162831989981328	35.28500601863633	21.308259970778877	18.243902020603464
9	25.64803986881946	15.430654158577486	20.511040838091535	38.41026513451152
10	15.458531452735835	28.240413784821598	33.64289043222672	22.65816433021584
11	32.49884916811295	24.214789547873178	24.89084967133385	18.395511612680025
12	22.16337809902587	25.831172247367384	30.56209202652203	21.443357627084723
13	28.00445750785568	21.593108732884055	26.860845125190497	23.54158863406977
14	21.978673155166447	21.078736915541807	28.061856141725304	28.88073378756644
15	23.538729424412505	28.800532956680115	24.256748449593562	23.403989169313817
16	22.910918463918204	27.667285209022523	25.064832578978518	24.356963748080755
17	22.100904368014593	28.193236825476703	26.45154926275279	23.254309543755916
18	23.329506757742024	26.64018561989095	27.354988034207583	22.67531958815944
19	24.438093822105696	26.35733830454586	27.462994679010826	21.741573194337622
20	23.881620142560195	26.36477224965475	27.217460049693067	22.536147558091994
21	23.87976165628297	26.453979590961467	26.951482071325845	22.71477668142972
22	23.050876776641402	27.204879527201093	27.197088180885036	22.54715551527247
23	23.281757956465672	26.206372034642182	27.337546855298257	23.174323153593885
24	22.766313935501948	26.966349961543628	27.058630953231912	23.208705149722515
25	23.159741184341826	26.72882111926621	27.60052266352535	22.510915032866617
26	23.400701078207963	26.992869131114777	27.310241403071362	22.2961883876059
27	23.397055585894947	27.074785487795467	26.748835586867077	22.779323339442513
28	22.782682910789802	26.46920488238641	27.678650567410156	23.069461639413632
29	23.392766771409047	26.59937040203347	27.40173611210389	22.60612671445359
30	23.412924199492778	26.56784761556211	27.451700800864625	22.567527384080492
31	23.487192170340272	26.2014398979834	27.00023159598224	23.311136335694087
32	22.408555327136472	26.856055949014575	27.673575470268506	23.061813253580446
33	23.06331433865051	27.013098039439935	27.239761885019742	22.68382573688981
34	23.02335688369021	26.577211527189654	27.280076741187205	23.119354847932936
35	23.10112738636786	26.714382110497013	28.12432987273658	22.060160630398546
36	23.834085782008138	27.145693887295675	26.01759271702116	23.002627613675028
37	23.194552061919044	26.434465485050623	27.27693161056421	23.094050842466128
38	23.59798654455935	26.33425018656343	27.037758722733862	23.030004546143353
39	22.80863023842949	26.480284319808316	27.564782542809517	23.146302898952673
40	22.989689689975897	26.01423314567387	28.182157388054797	22.813919776295435
41	22.908130734502368	25.752115100343964	27.501022167452472	23.838731997701196
42	22.73207489985618	26.483715371397036	27.380006118708668	23.404203610038113
43	23.169176576210802	25.38599330373098	28.09988363016695	23.344946489891264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	26.5
2	31.0
3	123.5
4	216.0
5	216.0
6	285.0
7	354.0
8	363.0
9	372.0
10	611.5
11	851.0
12	851.0
13	1360.5
14	1870.0
15	4392.5
16	6915.0
17	7703.5
18	8492.0
19	8492.0
20	8677.0
21	8862.0
22	10805.5
23	12749.0
24	17102.5
25	21456.0
26	21456.0
27	26542.0
28	31628.0
29	38205.0
30	44782.0
31	51787.0
32	58792.0
33	58792.0
34	66598.5
35	74405.0
36	80768.0
37	87131.0
38	92781.5
39	98432.0
40	98432.0
41	102113.0
42	105794.0
43	107094.0
44	108394.0
45	109040.0
46	109686.0
47	109686.0
48	107199.5
49	104713.0
50	104799.5
51	104886.0
52	97125.5
53	89365.0
54	89365.0
55	89914.5
56	90464.0
57	79384.0
58	68304.0
59	57151.0
60	45998.0
61	45998.0
62	42269.5
63	38541.0
64	30888.5
65	23236.0
66	19077.0
67	14918.0
68	14918.0
69	12471.5
70	10025.0
71	8768.0
72	7511.0
73	8448.0
74	9385.0
75	9385.0
76	8089.5
77	6794.0
78	4817.0
79	2840.0
80	1666.5
81	493.0
82	493.0
83	329.0
84	165.0
85	120.5
86	76.0
87	51.0
88	26.0
89	26.0
90	18.0
91	10.0
92	6.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1398988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.42731065437219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4566424411698	31.4659712026545
2	15.260097143269183	13.25409958513297
3	5.2013991989631565	6.776483365523271
4	2.253524831148035	3.914580916384294
5	1.2000115397491835	2.605663696275965
6	0.7110075115140093	1.8526286448066598
7	0.4493421562407936	1.3659604986422074
8	0.3274776586242979	1.1377179210755064
9	0.22864886951591226	0.893664493851468
>10	1.5398486560442366	13.320297742173889
>50	0.21372935877360266	6.563738823469654
>100	0.14760820179180145	12.055418540838657
>500	0.007996824897042783	2.279824782654826
>1k	0.0026656082990142607	2.5139497865160494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	4395	0.3141556610921609	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4361	0.31172533288348436	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4255	0.30414842729172803	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	3439	0.24582055028349062	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	2599	0.18577714748089333	RNA PCR Primer, Index 29 (95% over 23bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2573	0.1839186612036701	No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	2013	0.14388972600193856	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1759	0.12573374467829604	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1445	0.1032889488687537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.148024143166347E-5	0.0	0.0	0.0	0.0
6	7.148024143166347E-5	0.0	0.0	0.0	0.0
7	7.148024143166347E-5	0.0	0.0	0.0	0.0
8	7.148024143166347E-5	0.0	0.0	0.0	0.0
9	7.148024143166347E-5	0.0	0.0	1.4296048286332693E-4	0.0
10	1.4296048286332693E-4	0.0	0.0	1.4296048286332693E-4	0.0
11	1.4296048286332693E-4	0.0	0.0	2.1444072429499037E-4	0.0
12	1.4296048286332693E-4	0.0	0.0	2.1444072429499037E-4	0.0
13	1.4296048286332693E-4	0.0	0.0	2.8592096572665386E-4	0.0
14	1.4296048286332693E-4	0.0	0.0	2.8592096572665386E-4	0.0
15	1.4296048286332693E-4	0.0	0.0	3.574012071583173E-4	0.0
16	1.4296048286332693E-4	0.0	0.0	3.574012071583173E-4	7.148024143166347E-5
17	2.1444072429499037E-4	0.0	0.0	3.574012071583173E-4	7.148024143166347E-5
18	2.1444072429499037E-4	0.0	0.0	4.2888144858998074E-4	7.148024143166347E-5
19	2.1444072429499037E-4	0.0	0.0	4.2888144858998074E-4	7.148024143166347E-5
20	2.1444072429499037E-4	0.0	0.0	5.003616900216443E-4	7.148024143166347E-5
21	2.1444072429499037E-4	0.0	0.0	7.86282655748298E-4	7.148024143166347E-5
22	2.1444072429499037E-4	0.0	0.0	0.0012151641043382788	7.148024143166347E-5
23	2.1444072429499037E-4	0.0	0.0	0.001429604828633269	7.148024143166347E-5
24	2.1444072429499037E-4	0.0	0.0	0.00185848627722325	7.148024143166347E-5
25	2.1444072429499037E-4	0.0	0.0	0.002215887484381567	7.148024143166347E-5
26	2.1444072429499037E-4	0.0	0.0	0.0023588479672448943	7.148024143166347E-5
27	2.1444072429499037E-4	0.0	0.0	0.0030736503815615287	7.148024143166347E-5
28	2.1444072429499037E-4	0.0	0.0	0.009864273317569557	7.148024143166347E-5
29	2.1444072429499037E-4	0.0	0.0	0.023088117982427298	7.148024143166347E-5
30	2.1444072429499037E-4	0.0	0.0	0.043102585583293065	7.148024143166347E-5
31	2.8592096572665386E-4	0.0	0.0	0.0958550037598607	7.148024143166347E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGC	20	0.0018420727	37.0	15
GATTACG	95	0.0	33.105263	1
GGTATCA	775	0.0	26.735483	1
GTATCAA	2390	0.0	24.537657	1
TATACAC	980	0.0	23.408163	3
CTTATAC	950	0.0	22.78421	1
CGAACGC	90	3.8307917E-9	22.61111	30
ACGAACG	90	3.8307917E-9	22.61111	29
CCGTCTT	485	0.0	21.360825	37
TTATACA	1070	0.0	21.266356	2
GCCGTCT	495	0.0	20.929293	36
GCAACGC	110	1.7553248E-9	20.181818	3
CGTCTTC	225	0.0	19.733334	37
AGCAACG	95	1.6767626E-7	19.473684	2
TACGGAA	60	9.238183E-4	18.5	2
CGGCTAG	70	1.2194373E-4	18.5	21
ACGGTAC	240	0.0	17.729166	3
GCGGGTA	85	2.7238015E-5	17.411764	22
TTAGCGA	160	3.45608E-11	17.34375	27
CTAGCCG	75	2.0676712E-4	17.266666	13
>>END_MODULE