FastQCFastQC Report
Fri 10 Feb 2017
ERR1631121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706985
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT175842.4871814819267737No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG157632.2296088318705487No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT139081.9672270274475414TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC88351.2496729067802006No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC52810.7469748297347186RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC45740.6469727080489684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36800.5205202373459126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36320.5137308429457486No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG21020.2973188964405185No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18380.25997722723961614No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA14940.2113199007051069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14260.2017015919715411No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT12760.1804847344710284No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11250.15912643125384557No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10000.14144571667008493No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9910.14017270522005418No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8580.12136042490293288No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8290.11725849911950041No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7880.11145922473602694No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7790.11018621328599616No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7120.10070935026910048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG200.001841456737.021
GATTACG555.002221E-1033.6363641
CTTATAC19700.032.8680231
CGTCTTC7400.031.537
TTATACA21450.030.186482
TATACAC22600.028.5685843
GACGATT405.9369442E-527.75000214
GGTATCA7000.027.4857161
CCGTCTT22700.025.10132237
GCCGTCT23450.024.29850836
TGGGACG502.7006766E-422.211
TCGGTGT753.7365498E-722.233
GTCGGTG753.7365498E-722.232
TTAACGG855.1735697E-821.76470635
GTATCAA17500.021.3542861
TTAGACA450.003824372820.5555554
TACTCGC450.003824372820.55555520
AACGGCC1001.2876626E-820.3537
GGGACGA555.1404024E-420.18181812
CGGGCTG555.1404024E-420.18181817
Produced by FastQC (version 0.11.5)