##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631121.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706985
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27289687900026	31.0	31.0	34.0	28.0	34.0
2	31.273287269178272	31.0	31.0	34.0	28.0	34.0
3	30.71899969589171	31.0	31.0	34.0	26.0	34.0
4	34.910610550436004	37.0	35.0	37.0	32.0	37.0
5	35.107430850725265	37.0	35.0	37.0	32.0	37.0
6	35.31318203356507	37.0	35.0	37.0	33.0	37.0
7	35.31073785158101	37.0	35.0	37.0	33.0	37.0
8	35.35159869021266	37.0	35.0	37.0	33.0	37.0
9	37.1006032659816	39.0	37.0	39.0	33.0	39.0
10	36.91003486636916	39.0	37.0	39.0	33.0	39.0
11	36.976395538802095	39.0	37.0	39.0	33.0	39.0
12	36.896706436487335	39.0	37.0	39.0	33.0	39.0
13	36.99905514261265	39.0	37.0	39.0	33.0	39.0
14	38.01118269835994	40.0	37.0	41.0	33.0	41.0
15	38.02123383098651	40.0	37.0	41.0	33.0	41.0
16	38.038477478305765	40.0	37.0	41.0	33.0	41.0
17	37.99160944008713	40.0	37.0	41.0	33.0	41.0
18	38.083587346266185	40.0	37.0	41.0	33.0	41.0
19	38.08920557013232	40.0	37.0	41.0	33.0	41.0
20	38.09440228576278	40.0	37.0	41.0	33.0	41.0
21	38.06561383904892	40.0	37.0	41.0	33.0	41.0
22	37.9557133461106	40.0	37.0	41.0	33.0	41.0
23	37.96240938633776	40.0	37.0	41.0	33.0	41.0
24	37.915313620515285	40.0	37.0	41.0	33.0	41.0
25	37.85626710609136	40.0	37.0	41.0	33.0	41.0
26	37.73858851319335	39.0	37.0	41.0	33.0	41.0
27	37.65373664222013	39.0	37.0	41.0	32.0	41.0
28	37.649657347751365	39.0	37.0	41.0	32.0	41.0
29	37.607957736019856	39.0	37.0	41.0	32.0	41.0
30	37.47422646873696	39.0	37.0	41.0	32.0	41.0
31	37.43261455334979	39.0	36.0	41.0	32.0	41.0
32	37.323194975848146	39.0	36.0	41.0	32.0	41.0
33	37.21588435398205	39.0	36.0	40.0	31.0	41.0
34	37.16794981505973	39.0	36.0	40.0	31.0	41.0
35	37.1517839841015	39.0	36.0	40.0	31.0	41.0
36	37.07757590330771	39.0	36.0	40.0	31.0	41.0
37	37.02732165463199	39.0	36.0	40.0	31.0	41.0
38	36.986193483595834	39.0	36.0	40.0	31.0	41.0
39	36.85064180993939	39.0	36.0	40.0	30.0	41.0
40	36.70868405977496	39.0	35.0	40.0	30.0	41.0
41	36.669513497457515	39.0	35.0	40.0	30.0	41.0
42	36.58658104485951	39.0	35.0	40.0	30.0	41.0
43	35.85581165088368	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	13.0
11	16.0
12	19.0
13	15.0
14	11.0
15	12.0
16	14.0
17	16.0
18	27.0
19	49.0
20	107.0
21	183.0
22	326.0
23	615.0
24	1179.0
25	1997.0
26	3172.0
27	4924.0
28	7044.0
29	10020.0
30	13516.0
31	17944.0
32	22687.0
33	29951.0
34	39173.0
35	51991.0
36	72041.0
37	106303.0
38	173980.0
39	149622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.03357921313748	23.655947438771683	16.11745652312284	24.193016824967998
2	13.241157874636661	22.474027030276456	41.779245669992996	22.505569425093885
3	13.932544537720037	30.032037454825772	35.22026634228449	20.815151665169697
4	10.617481276123256	19.974539770999385	38.05597007008635	31.352008882791004
5	11.78865181015156	38.80153044265437	35.68194516149565	13.727872585698423
6	25.599553031535322	41.45194028161842	17.67081338359371	15.277693303252542
7	22.690014639631677	34.22745885697716	22.64291321598054	20.439613287410623
8	24.602502174727896	34.46254163808285	23.29625098127966	17.638705205909602
9	22.904870683253534	14.343868681796643	19.657701365658394	43.093559269291426
10	14.127173843858074	28.64912268294235	33.73027716288182	23.49342631031776
11	29.850138263188047	23.360042999497868	25.56249425376776	21.22732448354633
12	19.25316661598195	27.997199374809934	28.14897062879693	24.600663380411184
13	29.544191178030648	20.382186326442568	26.98755984921887	23.086062646307916
14	21.273577232897445	23.83275458460929	26.63804748332709	28.25562069916618
15	24.042377136714357	29.15266943428786	22.78181290975056	24.023140519247228
16	24.51084535032568	27.250083099358548	23.55962290571936	24.67944864459642
17	19.673118948775432	31.853151056953116	24.76346740029845	23.710262593973
18	24.114797343649442	24.769691011831934	26.004512118361777	25.110999526156846
19	24.767003543215203	26.97030347178512	26.3807577247042	21.881935260295478
20	21.28050807301428	25.225429110942947	26.84540690396543	26.64865591207734
21	24.219184282551964	25.306901843744917	27.85094450377306	22.622969369930054
22	19.68655629185909	31.70831064308295	26.283867408785195	22.321265656272764
23	22.237812683437415	26.67934963259475	27.981781791692896	23.101055892274942
24	23.91267141452789	27.39053869601194	28.022659603810546	20.674130285649625
25	22.104005035467512	29.130886793920663	26.442993840039037	22.322114330572784
26	24.4648754924079	28.335961866234786	27.74528455342051	19.4538780879368
27	20.003394697200083	29.956222550690608	26.810469811947918	23.22991294016139
28	19.329405857267126	26.93154734541751	31.30603902487323	22.43300777244213
29	22.813355304567988	25.44636732038162	27.967778665742554	23.772498709307836
30	20.71840279496736	27.776544056804596	28.949836276582953	22.555216871645083
31	21.194226185845526	26.16066818956555	30.93955317298104	21.705552451607886
32	23.03330339398997	26.16406288676563	28.186878080864513	22.61575563837988
33	19.232515541348118	30.41196064980162	30.31393876814925	20.041585040701005
34	20.022348423233876	28.732434210061037	30.0040312029251	21.241186163779997
35	22.656633450497534	25.750899948372314	32.512429542352386	19.08003705877777
36	22.387603697391036	28.52776225803942	25.58187231695156	23.502761727617983
37	21.27937650728092	26.983740814868774	29.179827011888516	22.557055665961794
38	21.016146028557888	24.902508539785146	29.738537592735348	24.34280783892162
39	23.75071606894064	25.56801063671789	27.793093205655	22.888180088686465
40	19.719796035276563	24.738855845597858	34.4428806834657	21.098467435659877
41	21.823518179310735	25.89998373374258	27.13155158878901	25.144946498157672
42	21.819274807810633	24.69727080489685	31.84056238816948	21.642891999123037
43	22.29891723303889	23.991881015863136	31.24337857238838	22.46582317870959
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	17.5
2	25.0
3	84.0
4	143.0
5	143.0
6	218.0
7	293.0
8	311.5
9	330.0
10	492.5
11	655.0
12	655.0
13	1089.5
14	1524.0
15	3738.0
16	5952.0
17	6450.5
18	6949.0
19	6949.0
20	7072.5
21	7196.0
22	8357.5
23	9519.0
24	11896.5
25	14274.0
26	14274.0
27	16495.0
28	18716.0
29	22045.5
30	25375.0
31	28183.0
32	30991.0
33	30991.0
34	33884.0
35	36777.0
36	39300.5
37	41824.0
38	43373.0
39	44922.0
40	44922.0
41	46225.0
42	47528.0
43	47877.5
44	48227.0
45	48849.5
46	49472.0
47	49472.0
48	51005.5
49	52539.0
50	65871.0
51	79203.0
52	60993.5
53	42784.0
54	42784.0
55	51902.0
56	61020.0
57	49670.5
58	38321.0
59	27563.5
60	16806.0
61	16806.0
62	13873.5
63	10941.0
64	8725.0
65	6509.0
66	5203.5
67	3898.0
68	3898.0
69	3058.0
70	2218.0
71	1643.5
72	1069.0
73	773.5
74	478.0
75	478.0
76	357.0
77	236.0
78	184.0
79	132.0
80	98.0
81	64.0
82	64.0
83	46.0
84	28.0
85	22.5
86	17.0
87	15.0
88	13.0
89	13.0
90	10.0
91	7.0
92	3.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	706985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.114897018037766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26732145589516	32.17952861536787
2	12.196187389436405	10.028899770587374
3	3.589314668237351	4.427229088497333
4	1.4716296261907231	2.420236021181
5	0.7971890313991722	1.6388172464943118
6	0.5158853951148916	1.2726344935955096
7	0.35550816534319063	1.023167712500978
8	0.29962039443872973	0.9855089329481783
9	0.2536419328541296	0.938561575387823
>10	1.8766630916331826	16.05965100243955
>50	0.23946125548220207	6.875412089652551
>100	0.12891401752812187	9.214519679442876
>500	0.003811973636584247	1.1786013543473444
>1k	0.003118887520841656	3.0130419294467226
>5k	6.930861157425903E-4	2.0112592744157536
>10k+	0.0010396291736138855	6.732931213694846
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	17584	2.4871814819267737	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	15763	2.2296088318705487	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	13908	1.9672270274475414	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	8835	1.2496729067802006	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	5281	0.7469748297347186	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	4574	0.6469727080489684	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3680	0.5205202373459126	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3632	0.5137308429457486	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	2102	0.2973188964405185	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1838	0.25997722723961614	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1494	0.2113199007051069	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1426	0.2017015919715411	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1276	0.1804847344710284	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	1125	0.15912643125384557	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1000	0.14144571667008493	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	991	0.14017270522005418	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	858	0.12136042490293288	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	829	0.11725849911950041	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	788	0.11145922473602694	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	779	0.11018621328599616	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	712	0.10070935026910048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.4144571667008493E-4	0.0
13	0.0	0.0	0.0	1.4144571667008493E-4	0.0
14	0.0	0.0	0.0	1.4144571667008493E-4	0.0
15	0.0	0.0	0.0	2.8289143334016986E-4	0.0
16	0.0	0.0	0.0	4.243371500102548E-4	0.0
17	0.0	0.0	0.0	4.243371500102548E-4	0.0
18	0.0	0.0	0.0	4.243371500102548E-4	0.0
19	0.0	0.0	0.0	4.243371500102548E-4	0.0
20	0.0	0.0	0.0	5.657828666803397E-4	0.0
21	0.0	0.0	0.0	8.486743000205096E-4	0.0
22	0.0	0.0	0.0	0.0016973486000410193	0.0
23	0.0	0.0	0.0	0.002121685750051274	0.0
24	0.0	0.0	0.0	0.003111805766741869	0.0
25	0.0	0.0	0.0	0.0033946972000820385	0.0
26	0.0	0.0	0.0	0.0038190343500922935	0.0
27	0.0	0.0	0.0	0.004526262933442718	0.0
28	0.0	0.0	0.0	0.0103255373169162	0.0
29	0.0	0.0	0.0	0.024753000417264866	0.0
30	0.0	0.0	0.0	0.04441395503440667	0.0
31	0.0	0.0	0.0	0.08911080150215352	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCG	20	0.0018414567	37.0	21
GATTACG	55	5.002221E-10	33.636364	1
CTTATAC	1970	0.0	32.868023	1
CGTCTTC	740	0.0	31.5	37
TTATACA	2145	0.0	30.18648	2
TATACAC	2260	0.0	28.568584	3
GACGATT	40	5.9369442E-5	27.750002	14
GGTATCA	700	0.0	27.485716	1
CCGTCTT	2270	0.0	25.101322	37
GCCGTCT	2345	0.0	24.298508	36
TGGGACG	50	2.7006766E-4	22.2	11
TCGGTGT	75	3.7365498E-7	22.2	33
GTCGGTG	75	3.7365498E-7	22.2	32
TTAACGG	85	5.1735697E-8	21.764706	35
GTATCAA	1750	0.0	21.354286	1
TTAGACA	45	0.0038243728	20.555555	4
TACTCGC	45	0.0038243728	20.555555	20
AACGGCC	100	1.2876626E-8	20.35	37
GGGACGA	55	5.1404024E-4	20.181818	12
CGGGCTG	55	5.1404024E-4	20.181818	17
>>END_MODULE