FastQCFastQC Report
Fri 10 Feb 2017
ERR1631120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268520
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT55570.43806956137861447No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG44040.34717623687446786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33780.26629457950998014No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT33740.26597925141109324TruSeq Adapter, Index 6 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.25328729543089584No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC25080.19771071800208118No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC23920.1885662031343613No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG14680.1157254122914893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG705.456968E-1231.7142871
GGTATCA6000.024.6666661
GCCGTCT5150.023.70873836
TATACAC11050.023.4389153
GTATCAA17700.023.4124321
CTTATAC10550.023.3222751
CCGTCTT5250.023.25714137
TTATACA11350.022.16742
TAGCCGT450.003826022620.55555737
TGACCCG450.003826022620.5555575
CGTCTTC2450.020.38775437
CGAACTA656.902852E-519.92307729
CCGGCGT759.267014E-619.73333423
TTACGGG1355.638867E-1119.1851843
CGACCGG500.007035274518.56
AGTGCGG609.237534E-418.510
TGCCGTC6850.017.82481835
CGCTATC904.4465392E-516.44444512
GTACTAA1606.2937033E-1016.18751
TATGCCG7550.016.17218633
Produced by FastQC (version 0.11.5)