##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631120.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1268520
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539333238734905	31.0	31.0	34.0	30.0	34.0
2	31.523936555986506	31.0	31.0	34.0	30.0	34.0
3	30.99499416643017	31.0	31.0	34.0	27.0	34.0
4	35.15586825592028	37.0	35.0	37.0	32.0	37.0
5	35.36011178381106	37.0	35.0	37.0	33.0	37.0
6	35.54763267429761	37.0	35.0	37.0	33.0	37.0
7	35.55566329265601	37.0	35.0	37.0	33.0	37.0
8	35.57898495884968	37.0	35.0	37.0	33.0	37.0
9	37.35079856841043	39.0	37.0	39.0	34.0	39.0
10	37.194009554441394	39.0	37.0	39.0	33.0	39.0
11	37.256462649386684	39.0	37.0	39.0	34.0	39.0
12	37.20658247406426	39.0	37.0	39.0	33.0	39.0
13	37.25668337905591	39.0	37.0	39.0	34.0	39.0
14	38.4501269195598	40.0	38.0	41.0	34.0	41.0
15	38.45985557973071	40.0	38.0	41.0	34.0	41.0
16	38.46687714817267	40.0	38.0	41.0	34.0	41.0
17	38.4461183111027	40.0	38.0	41.0	34.0	41.0
18	38.44953331441365	40.0	38.0	41.0	34.0	41.0
19	38.49689480654621	40.0	38.0	41.0	34.0	41.0
20	38.47541859805127	40.0	38.0	41.0	34.0	41.0
21	38.439848011856334	40.0	38.0	41.0	34.0	41.0
22	38.39911629300287	40.0	38.0	41.0	34.0	41.0
23	38.354107148488005	40.0	38.0	41.0	34.0	41.0
24	38.33930091760477	40.0	38.0	41.0	34.0	41.0
25	38.33364156654999	40.0	38.0	41.0	34.0	41.0
26	38.21774193548387	40.0	38.0	41.0	34.0	41.0
27	38.173105666445935	40.0	38.0	41.0	33.0	41.0
28	38.123814366348185	40.0	38.0	41.0	33.0	41.0
29	38.054725979882065	40.0	37.0	41.0	33.0	41.0
30	37.980504840286315	40.0	37.0	41.0	33.0	41.0
31	37.954213571721375	40.0	37.0	41.0	33.0	41.0
32	37.90314303282565	40.0	37.0	41.0	33.0	41.0
33	37.86367499132848	40.0	37.0	41.0	33.0	41.0
34	37.80216472739886	40.0	37.0	41.0	33.0	41.0
35	37.75400072525463	40.0	37.0	41.0	33.0	41.0
36	37.71567180651468	40.0	37.0	41.0	33.0	41.0
37	37.665625295620096	40.0	37.0	41.0	33.0	41.0
38	37.62504887585533	40.0	37.0	41.0	32.0	41.0
39	37.56431589568947	40.0	37.0	41.0	32.0	41.0
40	37.52209109828777	40.0	37.0	41.0	32.0	41.0
41	37.44122363068773	40.0	37.0	41.0	32.0	41.0
42	37.387795225932585	39.0	36.0	41.0	32.0	41.0
43	36.81731387758963	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	4.0
11	5.0
12	2.0
13	7.0
14	3.0
15	5.0
16	3.0
17	9.0
18	15.0
19	36.0
20	101.0
21	179.0
22	397.0
23	742.0
24	1349.0
25	2416.0
26	3846.0
27	6237.0
28	9276.0
29	13373.0
30	18753.0
31	25565.0
32	34023.0
33	44808.0
34	59745.0
35	81614.0
36	113449.0
37	170682.0
38	303230.0
39	378642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.73346892441585	21.368681613218556	14.820972471856967	24.076876990508627
2	14.377069340648946	22.033393245672123	40.65201967647337	22.937517737205564
3	14.985258411377037	27.687226058714092	33.34460631286854	23.98290921704033
4	12.429366505849336	18.211459023113548	39.91667454986914	29.442499921167975
5	12.845678428404755	38.47712294642576	35.66305615993441	13.014142465235077
6	28.71196354807177	39.959243843218864	18.042837322233783	13.285955286475579
7	25.65099486015199	32.689275691356855	24.01672815564595	17.643001292845206
8	24.986046731624256	35.658720398574715	21.531075584145302	17.824157285655726
9	25.453599470248793	15.62174817898023	20.643663481852865	38.28098886891811
10	15.160502002333429	28.47743827452464	33.94861728628638	22.41344243685555
11	32.187588686027816	23.972739885851226	25.395106107905274	18.444565320215684
12	21.96007946268092	25.934474821051307	30.7878472550689	21.317598461198877
13	27.635118090373034	21.730441774666538	27.261533125216786	23.37290700974364
14	21.620549932204458	21.42622899126541	28.150443035978935	28.802778040551193
15	23.155094125437518	29.21956295525494	24.254012550058334	23.371330369249204
16	22.70409611200454	27.971573171885346	25.16239397092675	24.16193674518336
17	21.666903162740834	28.64716362375051	26.873679563585913	22.812253649922745
18	22.934364456216695	27.044429729133164	27.418251190363573	22.60295462428657
19	23.90557500078832	26.930123293286666	27.384826411881562	21.77947529404345
20	23.46316967805001	26.922161258789775	27.24679153659383	22.367877526566392
21	23.679721249960583	26.932330589978875	26.96275975152146	22.425188408539086
22	22.77283763756188	27.45482924983445	27.47256645539684	22.299766657206824
23	22.859947024879386	26.752120581465018	27.055072052470596	23.332860341185004
24	22.693374956642387	27.54438242991833	27.238593006022764	22.52364960741652
25	23.209488222495505	26.95219626020875	27.48178980228928	22.356525715006466
26	22.9245892851512	27.152587267051366	27.630230504840288	22.292592942957146
27	23.233610822060356	27.314035253681457	27.054362564248102	22.397991360010092
28	22.44552707091729	27.041670608267903	27.804055119351684	22.708747201463122
29	22.94106517831804	26.714596537697478	27.851590830258882	22.4927474537256
30	23.205388957209976	26.765995017816035	27.456090562230003	22.572525462743986
31	22.9811906789014	27.35896950777284	27.257670356005427	22.40216945732034
32	22.69573991738404	26.698278308580075	28.00003153280989	22.605950241225994
33	22.66554725191562	26.970169961845304	27.795620092706464	22.56866269353262
34	22.790417179074826	27.456090562230003	27.28400025226248	22.46949200643269
35	23.468293759656923	26.510106265569327	28.279254564374227	21.742345410399523
36	23.6685271024501	26.99074512029767	26.657837479897832	22.682890297354398
37	22.91907104342068	26.89835398732381	27.508908018793555	22.673666950461957
38	23.27231734619872	26.637814145618517	27.520496326427647	22.569372181755114
39	22.62542175133226	26.82472487623372	27.550215999747742	22.99963737268628
40	22.64607574180935	26.061000220729667	28.784094850692142	22.508829186768832
41	22.49684671901113	26.310582410998645	27.654037776306247	23.538533093683977
42	22.448916847980325	26.542033235581624	27.866253586857127	23.14279632958093
43	22.828650711064864	25.4329454797717	28.653391353703526	23.085012455459903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	25.5
2	28.0
3	113.5
4	199.0
5	199.0
6	267.5
7	336.0
8	344.0
9	352.0
10	549.5
11	747.0
12	747.0
13	1116.5
14	1486.0
15	3561.5
16	5637.0
17	6602.5
18	7568.0
19	7568.0
20	8145.0
21	8722.0
22	11182.5
23	13643.0
24	18237.0
25	22831.0
26	22831.0
27	28719.0
28	34607.0
29	40574.0
30	46541.0
31	53087.5
32	59634.0
33	59634.0
34	66135.5
35	72637.0
36	77582.5
37	82528.0
38	86966.5
39	91405.0
40	91405.0
41	92300.5
42	93196.0
43	93274.5
44	93353.0
45	92574.0
46	91795.0
47	91795.0
48	90167.0
49	88539.0
50	90864.0
51	93189.0
52	85531.0
53	77873.0
54	77873.0
55	77890.0
56	77907.0
57	69664.5
58	61422.0
59	50824.0
60	40226.0
61	40226.0
62	37248.0
63	34270.0
64	27359.0
65	20448.0
66	16794.5
67	13141.0
68	13141.0
69	10926.5
70	8712.0
71	7595.0
72	6478.0
73	7830.0
74	9182.0
75	9182.0
76	7899.0
77	6616.0
78	4627.0
79	2638.0
80	1515.5
81	393.0
82	393.0
83	258.0
84	123.0
85	96.0
86	69.0
87	43.0
88	17.0
89	17.0
90	12.0
91	7.0
92	4.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1268520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.51516048337977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27727412532266	33.15473844757236
2	15.82031785382689	14.717692477376723
3	5.460631140706456	7.6200640155150605
4	2.4778829362789243	4.610364897601698
5	1.3440290958733254	3.1258864544439784
6	0.7779888933292204	2.171296693649743
7	0.5032188535087286	1.6385114010454622
8	0.372824990401301	1.3873611408584834
9	0.2560353736151843	1.0718573843819157
>10	1.4592406945442338	12.811894562875361
>50	0.14918268300395135	4.748828506767872
>100	0.09234346953271297	8.204869887148417
>500	0.006474260795141443	2.0352278997477593
>1k	0.0023852539771573736	2.261012109333888
>5k	1.7037528408266953E-4	0.44039412168133246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	5557	0.43806956137861447	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	4404	0.34717623687446786	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3378	0.26629457950998014	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	3374	0.26597925141109324	TruSeq Adapter, Index 6 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3213	0.25328729543089584	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	2508	0.19771071800208118	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2392	0.1885662031343613	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	1468	0.1157254122914893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.883202472172295E-5	0.0
5	0.0	0.0	0.0	7.883202472172295E-5	0.0
6	0.0	0.0	0.0	7.883202472172295E-5	0.0
7	0.0	0.0	0.0	7.883202472172295E-5	0.0
8	0.0	0.0	0.0	7.883202472172295E-5	0.0
9	0.0	0.0	0.0	1.576640494434459E-4	0.0
10	0.0	0.0	0.0	2.3649607416516885E-4	0.0
11	0.0	0.0	0.0	2.3649607416516885E-4	0.0
12	0.0	0.0	0.0	3.153280988868918E-4	0.0
13	0.0	0.0	0.0	3.941601236086148E-4	0.0
14	0.0	0.0	0.0	3.941601236086148E-4	0.0
15	0.0	0.0	0.0	3.941601236086148E-4	0.0
16	0.0	0.0	0.0	3.941601236086148E-4	0.0
17	0.0	0.0	0.0	3.941601236086148E-4	0.0
18	0.0	0.0	0.0	4.729921483303377E-4	0.0
19	0.0	0.0	0.0	4.729921483303377E-4	0.0
20	0.0	0.0	0.0	6.306561977737836E-4	0.0
21	0.0	0.0	0.0	7.094882224955066E-4	0.0
22	0.0	0.0	0.0	9.459842966606754E-4	0.0
23	0.0	0.0	0.0	0.0011824803708258443	0.0
24	0.0	0.0	0.0	0.0012613123955475672	0.0
25	0.0	0.0	0.0	0.0013401444202692902	0.0
26	0.0	0.0	0.0	0.0014978084697127361	0.0
27	0.0	0.0	0.0	0.0027591208652603034	0.0
28	0.0	0.0	0.0	0.011272979535206382	0.0
29	0.0	0.0	0.0	0.024595591713177562	0.0
30	0.0	0.0	0.0	0.04595907041276448	0.0
31	0.0	0.0	0.0	0.10516192097877843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	70	5.456968E-12	31.714287	1
GGTATCA	600	0.0	24.666666	1
GCCGTCT	515	0.0	23.708738	36
TATACAC	1105	0.0	23.438915	3
GTATCAA	1770	0.0	23.412432	1
CTTATAC	1055	0.0	23.322275	1
CCGTCTT	525	0.0	23.257141	37
TTATACA	1135	0.0	22.1674	2
TAGCCGT	45	0.0038260226	20.555557	37
TGACCCG	45	0.0038260226	20.555557	5
CGTCTTC	245	0.0	20.387754	37
CGAACTA	65	6.902852E-5	19.923077	29
CCGGCGT	75	9.267014E-6	19.733334	23
TTACGGG	135	5.638867E-11	19.185184	3
CGACCGG	50	0.0070352745	18.5	6
AGTGCGG	60	9.237534E-4	18.5	10
TGCCGTC	685	0.0	17.824818	35
CGCTATC	90	4.4465392E-5	16.444445	12
GTACTAA	160	6.2937033E-10	16.1875	1
TATGCCG	755	0.0	16.172186	33
>>END_MODULE