FastQCFastQC Report
Fri 10 Feb 2017
ERR1631119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472794
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT183983.891335338434921No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG152193.2189494790543027No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT122962.6007098228826933Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC85031.7984576792429683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53171.1245912596183538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51471.0886347965498717No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC46850.990917820446114Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC41450.8767031730521115No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21500.45474350351315795No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG19760.41794100601953493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19670.4160374285629682No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14500.3066874791135251No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA14120.2986501520746879No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT12530.26502028367534275No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11970.2531758017233721No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11320.23942774231483477No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10030.21214313210404534No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9950.2104510632537638No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9870.20875899440348228No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9490.20072166736464508No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8790.1859160649246818No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8630.18253192722411873No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8240.17428309157899635No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6640.140441714573366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.13663455966023258No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6360.13451947359738067No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5840.1235210260705508No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5560.11759878509456549No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5470.1156952076379988No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5440.11506068181914321No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5390.11400313878771728No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5350.11315710436257652No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5320.11252257854372096No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5170.1093499494494431No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5150.1089269322368727No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5060.10702335478030602No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4790.10131262241060589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCAA200.001840840337.01
CTTATAC21750.034.703451
GATTACG800.034.68751
TTATACA22400.033.448662
AGCAACG401.597431E-632.3752
CGTCTTC5600.032.37537
TATACAC23650.031.7589863
GACCGTT658.731149E-1131.3076927
GGTATCA5950.029.8487381
CGTTATT502.71506E-729.610
TGTTACG502.71506E-729.616
CGCCGTT702.0736479E-1029.0714325
CAGACAC454.0016985E-628.77777718
TTACGGG1000.027.7500023
GAAGCGC405.932847E-527.7536
GCACCTA405.932847E-527.7525
ACCTAAT405.932847E-527.7527
GTTATCG405.932847E-527.7511
CGTCAAA358.861641E-426.42857227
TACCGGG856.91216E-1126.11764732
Produced by FastQC (version 0.11.5)