##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472794
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332354048486234	31.0	31.0	34.0	28.0	34.0
2	31.33333967859152	31.0	31.0	34.0	28.0	34.0
3	30.787664395064237	31.0	31.0	34.0	26.0	34.0
4	34.95963146740441	37.0	35.0	37.0	32.0	37.0
5	35.17310710372805	37.0	35.0	37.0	32.0	37.0
6	35.38360892904732	37.0	35.0	37.0	33.0	37.0
7	35.37222342077099	37.0	35.0	37.0	33.0	37.0
8	35.41448918556496	37.0	35.0	37.0	33.0	37.0
9	37.19406549152485	39.0	37.0	39.0	34.0	39.0
10	37.02051633480966	39.0	37.0	39.0	33.0	39.0
11	37.09322453330626	39.0	37.0	39.0	33.0	39.0
12	36.976002233530885	39.0	37.0	39.0	33.0	39.0
13	37.08605016138107	39.0	37.0	39.0	33.0	39.0
14	38.03448436316874	40.0	37.0	41.0	33.0	41.0
15	38.047682500200935	40.0	37.0	41.0	33.0	41.0
16	38.07584064095568	40.0	37.0	41.0	33.0	41.0
17	38.04147895277859	40.0	37.0	41.0	33.0	41.0
18	38.12926771490332	40.0	37.0	41.0	33.0	41.0
19	38.106177320355165	40.0	37.0	41.0	33.0	41.0
20	38.14923412733664	40.0	37.0	41.0	33.0	41.0
21	38.08975156199106	40.0	37.0	41.0	33.0	41.0
22	37.97534021159321	40.0	37.0	41.0	33.0	41.0
23	37.9999598133648	40.0	37.0	41.0	33.0	41.0
24	37.88929428038427	40.0	37.0	41.0	33.0	41.0
25	37.86456680922347	40.0	37.0	41.0	33.0	41.0
26	37.6597757162739	39.0	37.0	41.0	32.0	41.0
27	37.5793411083897	39.0	37.0	41.0	32.0	41.0
28	37.59821402132853	39.0	37.0	41.0	32.0	41.0
29	37.472228919994755	39.0	36.0	41.0	32.0	41.0
30	37.363737695486826	39.0	36.0	41.0	32.0	41.0
31	37.275409586416075	39.0	36.0	41.0	32.0	41.0
32	37.11137197172553	39.0	35.0	41.0	31.0	41.0
33	36.97663675934974	39.0	35.0	40.0	31.0	41.0
34	36.89059294322686	39.0	35.0	40.0	31.0	41.0
35	36.88350952000237	39.0	35.0	40.0	31.0	41.0
36	36.73885666907786	39.0	35.0	40.0	30.0	41.0
37	36.62879182053918	39.0	35.0	40.0	30.0	41.0
38	36.58520624204199	39.0	35.0	40.0	30.0	41.0
39	36.40203344374083	39.0	35.0	40.0	30.0	41.0
40	36.20092894579881	39.0	35.0	40.0	29.0	41.0
41	36.12923598861238	39.0	35.0	40.0	29.0	41.0
42	35.97173610494211	38.0	35.0	40.0	28.0	41.0
43	35.12701514824638	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	11.0
11	11.0
12	7.0
13	15.0
14	15.0
15	7.0
16	11.0
17	23.0
18	20.0
19	45.0
20	87.0
21	157.0
22	271.0
23	529.0
24	911.0
25	1577.0
26	2314.0
27	3737.0
28	5349.0
29	7270.0
30	9914.0
31	12492.0
32	16005.0
33	20206.0
34	26671.0
35	35310.0
36	48430.0
37	71512.0
38	108667.0
39	101210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72022064577808	22.207769134126067	16.408626166998737	24.663384053097122
2	13.497844727301956	21.86998988988862	41.76300883682957	22.869156545979855
3	14.104662918734162	30.18100906525887	34.92557012144824	20.788757894558728
4	10.166161161097644	20.217684657588716	34.261856114925315	35.354298066388324
5	12.573552117835673	38.219393647127504	33.25401760597639	15.953036629060435
6	26.12977322047234	39.79470974673959	16.225671222562045	17.849845810226018
7	23.353088237160367	32.89424146668528	19.711756071354543	24.040914224799806
8	26.203589724065875	31.703871030512232	22.74415495966531	19.348384285756588
9	22.92182218894487	12.710609694708477	17.305211149041654	47.062356967305
10	15.861453402538949	27.655173289001127	30.432915815344526	26.0504574931154
11	30.927634445445584	21.87400855340804	23.910413414721845	23.287943586424532
12	20.401697145056833	27.71016552663528	24.435377775521687	27.452759552786205
13	32.36398939072831	18.839071561821854	25.376379564884495	23.420559482565345
14	22.69910362652656	23.154693164464863	25.124261306192547	29.021941902816025
15	26.400504236517385	26.63104861736824	21.28030389556551	25.68814325054887
16	26.484050136000032	24.135458571809288	21.354331907765328	28.026159384425352
17	20.048266263954282	31.371802518644483	22.059713109726435	26.520218107674804
18	25.583446490437694	21.83255286657614	24.522519321311183	28.06148132167498
19	24.664864613341116	27.28799434849004	24.917617397851917	23.129523640316922
20	22.177523403427283	22.08742073714979	25.829642508153654	29.90541335126926
21	25.65282131329924	22.80083926614974	27.499502954775227	24.046836465775794
22	20.649162214410506	31.01329543099109	24.883987529452572	23.453554825145837
23	26.015347064472056	21.813517092010475	26.20147463800302	25.969661205514456
24	23.09547075470501	27.12132556673731	25.663185235007212	24.120018443550467
25	20.856652157176274	27.34298658612419	28.16406299572329	23.636298260976236
26	23.767856614085627	29.35802907820319	26.72178580946459	20.152328498246593
27	19.914169807569472	28.614153309898178	26.74716684221881	24.72451004031354
28	20.259774870239468	23.381853407615154	31.93124278226881	24.427128939876564
29	19.950760796456805	26.79517929584555	25.79389755369146	27.460162354006183
30	22.510860966932743	22.50176609686248	30.861432251678323	24.125940684526455
31	22.500708553831057	22.916957491000307	28.557680512020035	26.024653443148605
32	20.990325596348516	23.25960143318232	31.022813318273922	24.727259652195247
33	23.166960663629403	25.78205307173949	30.2247913467599	20.826194917871206
34	19.274990799375626	27.134227591720705	27.170818580608046	26.419963028295623
35	19.96873902799105	23.09589377191758	37.784954969817726	19.150412230273652
36	22.911458267236895	27.646712944749723	24.407670148098326	25.03415863991506
37	21.823246487899592	25.842333024530767	29.12325452522663	23.21116596234301
38	21.271843551314102	22.23061206360487	29.980921923713076	26.516622461367955
39	25.30573569038524	22.909131672567757	27.076697250811137	24.708435386235866
40	19.690182193513454	22.338058435597745	36.47317859363697	21.498580777251828
41	23.15300109561458	23.70567308383778	25.587042136744543	27.554283683803092
42	23.169498766904827	22.303794041379543	32.32972499651011	22.19698219520552
43	23.751993468614238	21.364907338079586	31.53360660245265	23.349492590853522
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	14.0
2	15.0
3	108.5
4	202.0
5	202.0
6	287.0
7	372.0
8	419.0
9	466.0
10	697.0
11	928.0
12	928.0
13	1407.5
14	1887.0
15	4860.0
16	7833.0
17	7926.0
18	8019.0
19	8019.0
20	6479.0
21	4939.0
22	4023.5
23	3108.0
24	3327.0
25	3546.0
26	3546.0
27	4130.0
28	4714.0
29	5632.5
30	6551.0
31	7994.5
32	9438.0
33	9438.0
34	11681.0
35	13924.0
36	15196.0
37	16468.0
38	19226.5
39	21985.0
40	21985.0
41	23635.0
42	25285.0
43	27323.0
44	29361.0
45	31830.0
46	34299.0
47	34299.0
48	38669.5
49	43040.0
50	57365.5
51	71691.0
52	54720.0
53	37749.0
54	37749.0
55	46580.0
56	55411.0
57	45364.5
58	35318.0
59	25028.0
60	14738.0
61	14738.0
62	12357.5
63	9977.0
64	7712.0
65	5447.0
66	4305.5
67	3164.0
68	3164.0
69	2428.5
70	1693.0
71	1213.5
72	734.0
73	492.5
74	251.0
75	251.0
76	170.0
77	89.0
78	65.0
79	41.0
80	32.0
81	23.0
82	23.0
83	21.5
84	20.0
85	25.5
86	31.0
87	19.0
88	7.0
89	7.0
90	9.5
91	12.0
92	7.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	472794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.6410814108312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.47997478300991	17.859849592831395
2	11.398393642402308	5.6173749139027205
3	4.2227147633395266	3.1215677477450425
4	2.2825393201701365	2.249769488469425
5	1.48806281754179	1.833373851573905
6	1.0414793115542351	1.5397905902222604
7	0.8347166282509181	1.4397824274193773
8	0.6340353666303523	1.2498653669187774
9	0.5276414906387024	1.1701491233904535
>10	4.242755721801203	21.115752258045966
>50	0.5250464243665265	9.01483551587425
>100	0.29150023889871407	12.459208182537637
>500	0.016434731569957173	2.7320525273076286
>1k	0.0095148445931331	4.767983697602719
>5k	0.0025949576163090274	4.042661903997799
>10k+	0.0025949576163090274	9.785982812160645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	18398	3.891335338434921	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	15219	3.2189494790543027	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	12296	2.6007098228826933	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	8503	1.7984576792429683	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5317	1.1245912596183538	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5147	1.0886347965498717	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	4685	0.990917820446114	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	4145	0.8767031730521115	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2150	0.45474350351315795	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	1976	0.41794100601953493	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1967	0.4160374285629682	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1450	0.3066874791135251	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	1412	0.2986501520746879	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	1253	0.26502028367534275	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1197	0.2531758017233721	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1132	0.23942774231483477	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1003	0.21214313210404534	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	995	0.2104510632537638	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	987	0.20875899440348228	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	949	0.20072166736464508	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	879	0.1859160649246818	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	863	0.18253192722411873	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	824	0.17428309157899635	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	664	0.140441714573366	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	646	0.13663455966023258	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	636	0.13451947359738067	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	584	0.1235210260705508	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	556	0.11759878509456549	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	547	0.1156952076379988	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	544	0.11506068181914321	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	539	0.11400313878771728	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	535	0.11315710436257652	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	532	0.11252257854372096	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	517	0.1093499494494431	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	515	0.1089269322368727	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	506	0.10702335478030602	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	479	0.10131262241060589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	4.2301721257037946E-4	0.0
12	0.0	0.0	0.0	4.2301721257037946E-4	0.0
13	0.0	0.0	0.0	4.2301721257037946E-4	0.0
14	0.0	0.0	0.0	6.345258188555692E-4	0.0
15	0.0	0.0	0.0	6.345258188555692E-4	0.0
16	0.0	0.0	0.0	8.460344251407589E-4	0.0
17	0.0	0.0	0.0	8.460344251407589E-4	0.0
18	0.0	0.0	0.0	0.0010575430314259486	0.0
19	0.0	0.0	0.0	0.0010575430314259486	0.0
20	0.0	0.0	0.0	0.0010575430314259486	0.0
21	0.0	0.0	0.0	0.0012690516377111384	0.0
22	0.0	0.0	0.0	0.0027496118817074665	0.0
23	0.0	0.0	0.0	0.0035956463068482257	0.0
24	0.0	0.0	0.0	0.0042301721257037945	0.0
25	0.0	0.0	0.0	0.004653189338274175	0.0
26	0.0	0.0	0.0	0.004864697944559364	0.0
27	0.0	0.0	0.0	0.006133749582270503	0.0
28	0.0	0.0	0.0	0.018824265959381886	0.0
29	0.0	0.0	0.0	0.04843547083930845	0.0
30	0.0	0.0	0.0	0.0843919339077907	0.0
31	0.0	0.0	0.0	0.14784451579334762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCAA	20	0.0018408403	37.0	1
CTTATAC	2175	0.0	34.70345	1
GATTACG	80	0.0	34.6875	1
TTATACA	2240	0.0	33.44866	2
AGCAACG	40	1.597431E-6	32.375	2
CGTCTTC	560	0.0	32.375	37
TATACAC	2365	0.0	31.758986	3
GACCGTT	65	8.731149E-11	31.307692	7
GGTATCA	595	0.0	29.848738	1
CGTTATT	50	2.71506E-7	29.6	10
TGTTACG	50	2.71506E-7	29.6	16
CGCCGTT	70	2.0736479E-10	29.07143	25
CAGACAC	45	4.0016985E-6	28.777777	18
TTACGGG	100	0.0	27.750002	3
GAAGCGC	40	5.932847E-5	27.75	36
GCACCTA	40	5.932847E-5	27.75	25
ACCTAAT	40	5.932847E-5	27.75	27
GTTATCG	40	5.932847E-5	27.75	11
CGTCAAA	35	8.861641E-4	26.428572	27
TACCGGG	85	6.91216E-11	26.117647	32
>>END_MODULE